Fluidigm launch Customer Web Videos

Fluidigm has added a new genre - customer web videos (www.fluidigm.com/biomark-videos.html) to the wide array of topical information (publications, application notes, posters, newsletters, press releases) available from its website.

Eve Marquis, Marketing Manager of Fluidigm Europe commented "Online video creates a more personal interaction between Fluidigm and our target audience in Single Cell, Gene Expression, SNP Genotyping, Next Generation Sequencing and Copy Number Variation markets. The use of both visual effects and commentary from existing customers is a great way of effectively communicating the value of implementing Fluidigm technological solutions in your lab. In today's fast paced world, it is much more efficient for someone to click on a video link than it is for them to read a page of text. People want fast and easy information, and web video allows them to have that".

The first customer web video from Dr Paul Robson from the Genome Insitute of Singapore reports on how he and fellow scientists have generated significant single cell expression data crucial for a detailed molecular understanding of mammalian development from fertilization to embryo implantation, a process known as the preimplantation period. The knowledge gained has a direct impact on clinical applications in the areas of regenerative medicine and assisted reproduction.

A further customer web video from Professor Mylene Yao of Stanford University reports upon her group's pioneering work using Fluidigm IFC technology to undertake single embryo gene expression has allowed them to gain a better understanding of early embryo development.

Social Media Pages for Dynamic Light Scattering

Avid Nano has announced that it has launched Facebook, LinkedIn and Twitter pages to facilitate simple communication about topics involving Dynamic Light Scattering via its website - http://www.avidnano.com/.

Visitors to the social media pages can learn about the latest innovations and applications advances in dynamic light scattering technology, obtain information on industry trends and events, and share feedback with Avid Nano and the life sciences community.

Maria Cunningham of Avid Nano commented 'Our aim in establishing and maintaining the social media pages is to facilitate two-way dialog and flow of information about topics of interest to the DLS community'. She added 'We welcome comments and suggestions to help improve this new facility'.

Dynamic light scattering is a non-invasive technique that enables quick and accurate measurement of hydrodynamic radius, size distribution, estimated molecular weight and aggregate content of biomolecules and nanoparticles in solution or suspension.  Avid Nano DLS systems find particular utility in applications including crystal screening, buffer optimisation, solubility screening, aggregate detection, micelle formation, engineered nanoparticles and formulation development.

Operating from its UK headquarters - Avid Nano is building a strong reputation for its technical expertise, applications support and delivering DLS systems that reliably deliver top quality results.  For further information please contact Avid Nano via its website or telephone +44-1494-614659 / email info@avidnano.com.

Just Published: Journal of Chromatography A

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

http://rss.sciencedirect.com/publication/science/5248

Selected papers from the latest issue:

Dispersive liquid-liquid microextraction using non-chlorinated, lighter than water solvents for gas chromatography-mass spectrometry determination of fungicides in wine

24 July 2011, 20:44:11

Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 24 July 2011
T., Rodríguez-Cabo , I., Rodríguez , M., Ramil , R., Cela.
A novel and low solvent consumption method for the sensitive determination of fungicide residues in wine samples is proposed. Analytes were extracted by dispersive liquid-liquid microextraction (DLLME) and further determined by gas chromatography-mass spectrometry (GC-MS). Under optimized conditions, a binary mixture of acetone and 1-undecanol (0.5 and 0.05mL, respectively) was used to extract target compounds from diluted (1:1) wine samples. After centrifugation, the floating phase of 1-undecanol was solidified and separated from the liquid hydro-alcoholic matrix. Thereafter, it was allowed to melt at room temperature and injected in the GC-MS system. The method showed relative standard deviations (RSDs, %) below...

High performance liquid chromatopgraphy column packings with deliberately broadened particle size distribution: relation between column performance and packing structure

24 July 2011, 20:44:11

Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 24 July 2011
Anuschka, Liekens , Jeroen, Billen , Ron, Sherant , Harald, Ritchie , Joeri, Denayer , ...
The effect of the addition of 25%, 50% and 75% (weight percent, wt%) of larger particles (resp. 3 and 5μm) to a commercial batch of 1.9μm particles has been investigated as an academic exercise to study the effects of particle size distribution on the kinetic performance of packed bed columns in a magnified way. Comparing the performance of the different mixture in a kinetic plot, it could be irrefutably shown that the addition of larger particles to a commercial batch of small particles can not be expected to lead to an improved kinetic performance. Whereas the addition of 25 wt%...

Development of a multi-residue method for the determination of organic micropollutants in water, sediment and mussels using gas chromatography-tandem mass spectrometry

24 July 2011, 20:44:11

Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 24 July 2011
Juan, Sánchez-Avila , María, Fernandez-Sanjuan , Joana, Vicente , Silvia, Lacorte
This study describes the development of a multiresidue method based in gas chromatography-electron ionization-tandem mass spectrometry (GC-EI-MS/MS) for the detection of sixteen polycyclic aromatic hydrocarbons (PAHs), five phthalate esters (PEs), seven polychlorinated biphenyls (PCBs), six polybrominated diphenyl ethers (PBDEs), six alkylphenols (APs), three organochlorined pesticides and their isomers or degradation products (OCPs) and bisphenol A in seawater, river water, wastewater treatment plant (WWTP) effluents, sediments and mussels. Solid phase extraction (SPE) was used for the extraction of target analytes in aqueous samples, and ultrasound assisted extraction for solid samples. GC-EI-MS/MS acquisition conditions in selected reaction monitoring (SRM) using two transitions...

Simultaneous Determination of Flonicamid and Its Metabolites in Vegetables Using QuEChERS and Reverse-Phase Liquid Chromatography Tandem Mass Spectrometry

24 July 2011, 20:44:11

Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 24 July 2011
Yong, Xu , Lin-Fei, Shou , Yin-Liang, Wu
This paper presents a rapid analytical method for the simultaneous determination of flonicamid and its metabolites N-(4-trifluoromethylnicotinoyl) glycine (TFNG), 4-trifluoromethylnicotinic acid (TFNA), and 4-trifluoromethylnicotinamide (TFNA-AM) in vegetables using QuEChERS by liquid chromatography tandem mass spectrometry (LC-MS/MS). Samples were extracted with acetonitrile. The extract was purified through QuEChERS method with primary secondary amine (PSA) and graphite carbon black (GCB). Then the extract was diluted with 0.1% formic acid in water, and analyzed by LC-MS/MS on a Waters Acquity BEH C18 column with methanol/0.1% formic acid in water as mobile phase with gradient elution. The linearity of the analytical response across the...

Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

http://rss.sciencedirect.com/publication/science/7220

Selected papers from the latest issue:

Development and validation of an EI-GC/MS method for the determination of sertraline and its major metabolite desmethyl-sertraline in blood

21 July 2011, 21:49:43

Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Alaa, Khraiwesh , Ioannis, Papoutsis , Panagiota, Nikolaou , Constantinos, Pistos , Chara, Spiliopoulou , ...
Α sensitive and specific GC/MS method for the determination of sertraline and its main metabolite desmethyl-sertraline in whole blood has been developed, optimized and validated. Sample preparation included solid-phase extraction of both analytes and their derivatization with heptafluorobutyric anhydride (HFBA). Protriptyline was used as internal standard for the determination of both analytes. Limits of detection and quantification for both sertraline and desmethyl-sertraline were 0.30 and 1.00μg/L, respectively. The calibration curves were linear within the dynamic range of each analyte (1.00-500.0μg/L) with a correlation coefficient (R2) exceeding 0.991. Extraction efficiency ranged from 90.1(±5.8) to 95.4(±3.0) % for sertraline, and from 84.9(±8.2)...

The Effects of Hitchhiker Antigens Co-Eluting with Affinity-Purified Research Antibodies

21 July 2011, 21:49:43

Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Lilly, Mechetner , Radhika, Sood , Van, Nguyen , Pete, Gagnon , Missag H., Parseghian
The popularity of Protein G for the purification of antibodies has given rise to an entire industry that supplies scientists with research grade immunoreagents; however, many times the supplied product is contaminated with antigens bound to the antibody's complementarity-determining regions (CDRs). These “hitchhikers” are a category of host cell proteins that are elusive to detect due to their interaction with the antibody in the final product and yet their impact on an experiment or an entire field of study can be far reaching. In an earlier work, the role of hitchhikers on a human anti-histone antibody destined for clinical usage...

Self-assembly molecularly imprinted polymers of 17β-Estradiol on the surface of magnetic nanoparticles for selective separation and detection of estrogenic hormones in feeds

21 July 2011, 21:49:43

Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Shu, Wang , Yun, Li , Meijuan, Ding , Xiaoli, Wu , Jinhui, Xu , ...
This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17β-Estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation...

COMPARATIVE STUDY OF LC-MS/MS AND UPLC-MS/MS FOR MULTI-RESIDUE ANALYSIS OF QUINOLONES, PENICILLINS AND CEPHALOSPORINS IN COW MILK, AND VALIDATION ACCORDING TO THE REGULATION 2002/657/EC

21 July 2011, 21:49:43

Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
A., Junza , R., Amatya , D., Barrón , J., Barbosa
The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated β-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya,...

Accurate & Precise Micro Determination of Fluorine

Operating in an ISO 17025 and GMP accredited environment, Warwick Analytical Service has developed proprietary analytical procedures to enable accurate and precise data to be produced for all types of samples including organic samples with trace levels of Fluorine. 

From extensive method validation typical results are within the ±0.3% absolute limits considered acceptable for micro chemical analysis. The WAS method can detect 0.01ppm fluoride which is equivalent to 0.02% in a 10mg sample.  WAS has developed special procedures to remove interferences from samples containing boron, calcium or sulfur.

Offered by Warwick Analytical Service (WAS) as an analytical service, analysis of Fluorine is carried out by combustion of an accurately weighed sample in a silica oxygen flask over an accurately measured volume of water and TISAB buffer solution.  A fluoride ion selective electrode is used to directly measure the potential of a range of fluoride solutions.  The potential of the combusted samples are measured under the same conditions.  The concentrations of the sample solutions are calculated from the derived calibration curve. Sets of work typically include certified reference materials or organic analytical standards.  Results from these standards and the electrode response are monitored to detect trends in the method.

The accurate micro determination of fluorine in organic compounds is of significant interest to a wide array of industries including food and beverages, environmental monitoring, medical, pharmaceutical and polymers.

Warwick Analytical Service (WAS) is a company dedicated to providing analytical excellence at a realistic cost. Through experienced staff, the latest analytical instrumentation and working closely with clients - WAS has established a strong reputation for informed personal service and delivering top quality data on time. Operating from a dedicated facility approved to BSI ISO 9001 and with a wide range of analytical techniques accredited to UKAS ISO17025 - WAS has become the trusted analytical services provider for a growing number of UK and international businesses. From its modern, well-equipped facility in Coventry, UK - Warwick Analytical Services offers a wide range of analytical techniques including elemental analysis (ICP, CHNS, Halogen analysis), Optical Rotation, NMR, MS, FT-IR, Thermal Analysis and Particle sizing on a contract basis.

For full details of micro determination of fluorine in organic compounds please contact Warwick Analytical Service on tel. +44-2476-323223 or email info@warwickanalytical.co.uk

Just Published: Sensors & Actuators B: Chemical

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

http://rss.sciencedirect.com/publication/science/5283

Selected papers from the latest issue:

Water diffusion in polymer nano-films measured with microcantilevers

20 July 2011, 23:14:24

Publication year: 2011
Source: Sensors and Actuators B: Chemical, In Press, Accepted Manuscript, Available online 20 July 2011
Chuanjun, Liu , Marcus C., Lopes , Sascha A., Pihan , Daniela, Fell , Mordechai, Sokuler , ...
We present a method to measure the absorption of water molecules from the liquid and the vapour phase into polymer nanofilms and the diffusion inside these films. Film thickness can be down to 45nm. To demonstrate the possibilities of this method we use polymer films that are deposited on the upper side of a silicon cantilever by plasma polymerization of norbornene. When a microdrop of water is deposited onto the initially straight cantilever, the drop causes the cantilever to bend while it evaporates. Evaporation of such small water drops usually takes less than a second. An upwards bending is due...

Plasma Enhanced-CVD of undoped and fluorine-doped Co3O4 nanosystems for novel gas sensors

20 July 2011, 23:14:24

Publication year: 2011
Source: Sensors and Actuators B: Chemical, In Press, Accepted Manuscript, Available online 20 July 2011
Davide, Barreca , Daniela, Bekermann , Elisabetta, Comini , Anjana, Devi , Roland A., Fischer , ...
Co3O4-based nanosystems were prepared on polycrystalline Al2O3 by plasma enhanced-chemical vapor deposition (PE-CVD), at temperatures ranging between 200 and 400°C. The use of two different precursors, Co(dpm)2 (dpm=2,2,6,6-tetramethyl-3,5-heptanedionate) and Co(hfa)2•TMEDA (hfa=1,1,1,5,5,5-hexafluoro-2,4-pentanedionate; TMEDA=N,N,N’,N’-tetramethylethylenediamine) enabled the synthesis of undoped and fluorine-doped Co3O4 specimens, respectively. A thorough characterization of their properties was performed by glancing incidence X-ray diffraction (GIXRD), atomic force microscopy (AFM), field emission-scanning electron microscopy (FE-SEM), secondary ion mass spectrometry (SIMS) and X-ray photoelectron spectroscopy (XPS). For the first time, the gas sensing properties of such PE-CVD nanosystems were investigated in the detection of ethanol and acetone. The results show an...

Preparation of ZnO Nanorods by Microemulsion Synthesis and Their Application as a CO Gas Sensor

20 July 2011, 23:14:24

Publication year: 2011
Source: Sensors and Actuators B: Chemical, In Press, Accepted Manuscript, Available online 20 July 2011
Sang Kyoo, Lim , Sung-Ho, Hwang , Soonhyun, Kim , Hyunwoong, Park
Zinc oxide nanorods with different surface area were synthesized by surfactant assisted microemulsion method. The alkyl chain length of surfactant would affect aspect ratio of ZnO nanorods. ZnO nanorods synthesized by ethyl benzene acid sodium salt (EBS), which is surfactant with short alkyl chain length, show higher aspect ratio than ones by dodecyl benzene sulfonic acid sodium salt (DBS). These nanorods had diameters in the range of 80 ∼ 300nm and length of up to several microns. The Brunauer-Emmett-Teller (BET) surface area of the ZnO nanorods was strongly affected by the morphology of the nanorods. The BET surface area of...

CE method development and validation course

CE method development and validation course on 22 and 23 November 2011 in Amsterdam. Please forward this invitation to anyone it may concern.

This course aims for analytical scientists and technicians who want a better understanding of the capillary electrophoresis techniques and practical approach for method development and validation. We’ll work from the understanding of the separation mechanisms in CE in order to rationally develop robust and reliable test methods. Simultaneously, a better understanding of the need and requirements for validation will help select appropriate specifications and tests for the validation protocol.

Deadline for early registration is 15 Sep 2011. Link to Registration form. Limited space available.

Kantisto BV is a pharmaceutical analytical chemistry training and consultancy with:

•       Focus on implementation and good working practices

•       On-the-job training and coaching

•       Courses and workshops

•       A pragmatic, scientific and personal approach

•       Specialties: Separation techniques, CE, CE-MS, Dissolution, Chiral separations, Quality by Design QbD, Application development, validation and transfer, Troubleshooting, Support on CMC and Regulatory documentation, GLP, GMP.

More information on www.kantisto.nl.