Rapid determination of DNPH derivatized

This application note describes a very fast method for identifying six DNPH derivatized

carbonyls in a mixture using the KNAUER PLATINblue UHPLC system. The stationary phase

BlueOrchid C18 A was used to separate the carbonyl mixture. Short columns with small

particles are the most suitable way for avoiding long equilibration and analysis times.

Reduction of analysis time from about 20 to less than 3 minutes compared to a

conventional HPLC method is achieved by using the BlueOrchid C18 A phase with a

1.8 μm particle size. A binary high pressure gradient configuration was used at a flow rate

of 0.8 ml/min in combination with a 2 mm column ID and UV detection

See more at: http://www.knauer.net/dwnld_fls/a_e_vev3_uhplc_dnph_carbonyls.pdf?k=21021-1