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Slected papers from the latest issue:Determination of Zoledronic Acid in Human Urine and Blood Plasma Using Liquid Chromatography/Electrospray Mass Spectrometry
01 June 2011, 21:47:00
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Katrin, Veldboer , Torsten, Vielhaber , Helmut, Ahrens , Jendrik, Hardes , Arne, Streitbürger , ...
A new method for the analysis of 1-hydroxy-2-imidazol-1-yl-phosphonoethyl phosphoric acid (zoledronic acid) in urine and blood samples has been developed. It consists of a derivatisation of the bisphosphonate with trimethylsilyl diazomethane under multiple methylester formation. The formed derivative can, in contrast to the non-derivatised analyte, easily be separated by reversed phase liquid chromatography due to its reduced polarity. Detection is performed by electrospray tandem mass spectrometry. For calibration purposes, a deuterated internal standard has been synthesised in a three-step synthesis starting with d4-imidazole. For human urine, the limit of detection (LOD) is 1.2 10−7mol/L, limit of quantification (LOQ) is 3.75...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Katrin, Veldboer , Torsten, Vielhaber , Helmut, Ahrens , Jendrik, Hardes , Arne, Streitbürger , ...
A new method for the analysis of 1-hydroxy-2-imidazol-1-yl-phosphonoethyl phosphoric acid (zoledronic acid) in urine and blood samples has been developed. It consists of a derivatisation of the bisphosphonate with trimethylsilyl diazomethane under multiple methylester formation. The formed derivative can, in contrast to the non-derivatised analyte, easily be separated by reversed phase liquid chromatography due to its reduced polarity. Detection is performed by electrospray tandem mass spectrometry. For calibration purposes, a deuterated internal standard has been synthesised in a three-step synthesis starting with d4-imidazole. For human urine, the limit of detection (LOD) is 1.2 10−7mol/L, limit of quantification (LOQ) is 3.75...
DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY METHOD FOR QUANTITATIVE DETERMINATION OF AMOXICILLIN IN BOVINE MUSCLE
01 June 2011, 21:47:00
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
B., Lugoboni , T., Gazzotti , E., Zironi , A., Barbarossa , G., Pagliuca
A simple, quick and economical liquid chromatographic/tandem mass spectrometry (LC-MS/MS) method for the quantitative determination of amoxicillin in bovine muscle was developed and validated.The sample preparation procedure involved a liquid extraction with water, followed by a protein precipitation step with acetonitrile. The extract was purified by a liquid-liquid partition with dichloromethane and the upper aqueous layer was directly injected into the LC-MS/MS system.Chromatographic separation was achieved on a reversed phase column, using a mixture of acetonitrile, water and 0.005% formic acid in water as mobile phase. Gradient elution was performed at a flow rate of 0.2mLmin−1. Amoxicillin was detected using...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
B., Lugoboni , T., Gazzotti , E., Zironi , A., Barbarossa , G., Pagliuca
A simple, quick and economical liquid chromatographic/tandem mass spectrometry (LC-MS/MS) method for the quantitative determination of amoxicillin in bovine muscle was developed and validated.The sample preparation procedure involved a liquid extraction with water, followed by a protein precipitation step with acetonitrile. The extract was purified by a liquid-liquid partition with dichloromethane and the upper aqueous layer was directly injected into the LC-MS/MS system.Chromatographic separation was achieved on a reversed phase column, using a mixture of acetonitrile, water and 0.005% formic acid in water as mobile phase. Gradient elution was performed at a flow rate of 0.2mLmin−1. Amoxicillin was detected using...
Determination of Octreotide and Assessment of Matrix Effects in Human Plasma Using Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry
01 June 2011, 21:47:00
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Omnia A., Ismaiel , Tianyi, Zhang , Rand, Jenkins , H. Thomas, Karnes
A selective UHPLC-MS/MS method for determination of the therapeutic peptide octreotide in human plasma was developed and validated. This assay used a UHPLC C 18 column with 1.7μm particle size for efficient separation and an ion-exchange SPE for selective extraction. Octreotide and its labeled internal standard, [13C6Phe3] octeotide, were extracted from human plasma using a simple Oasis® WCX μ-Elution SPE method and analyzed with a total chromatographic run time of 7.5min. Matrix effects were studied during method development by direct monitoring of representative phospholipids. On-line removal of phospholipids using column switching and pre-column back-flushing was carried out to trap and...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Omnia A., Ismaiel , Tianyi, Zhang , Rand, Jenkins , H. Thomas, Karnes
A selective UHPLC-MS/MS method for determination of the therapeutic peptide octreotide in human plasma was developed and validated. This assay used a UHPLC C 18 column with 1.7μm particle size for efficient separation and an ion-exchange SPE for selective extraction. Octreotide and its labeled internal standard, [13C6Phe3] octeotide, were extracted from human plasma using a simple Oasis® WCX μ-Elution SPE method and analyzed with a total chromatographic run time of 7.5min. Matrix effects were studied during method development by direct monitoring of representative phospholipids. On-line removal of phospholipids using column switching and pre-column back-flushing was carried out to trap and...
Direct Monitoring Changes of Salbutamol Concentration in Serum by Chemiluminescent Imaging
01 June 2011, 21:47:00
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Canli, Zhang , Ruichao, Zhang , Na, Na , Joris R., Delanghe , Jin, Ouyang
We report in this manuscript, the use of direct ammonium persulfate-enhanced chemiluminescence (CL) imaging, to monitor changes to measure serum salbutamol concentration in subjects of different haptoglobin (Hp) phenotypes at different dosing time. It was noted that CL generated from Hp was decreased due to salbutamol's reducibility, which was used for monitoring salbutamol concentration in serum. The serum from the subjects treated by oral administration of salbutamol, was collected at different dosing time and was separated by polyacrylamide gel electrophoresis (PAGE) prior to the CL detection. According to CL images, samples were separated into three groups based on the Hp...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 1 June 2011
Canli, Zhang , Ruichao, Zhang , Na, Na , Joris R., Delanghe , Jin, Ouyang
We report in this manuscript, the use of direct ammonium persulfate-enhanced chemiluminescence (CL) imaging, to monitor changes to measure serum salbutamol concentration in subjects of different haptoglobin (Hp) phenotypes at different dosing time. It was noted that CL generated from Hp was decreased due to salbutamol's reducibility, which was used for monitoring salbutamol concentration in serum. The serum from the subjects treated by oral administration of salbutamol, was collected at different dosing time and was separated by polyacrylamide gel electrophoresis (PAGE) prior to the CL detection. According to CL images, samples were separated into three groups based on the Hp...
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