A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from this issue:Computational investigation of the substrate recognition mechanism of protein d-aspartyl (l-isoaspartyl) O-methyltransferase by docking and molecular dynamics simulation studies and application to interpret size exclusion chromatography data
21 June 2011, 21:42:29
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Ikuhiko, Noji , Akifumi, Oda , Kana, Kobayashi , Ohgi, Takahashi
Unusual amino acid residues such as l-β-aspartyl (Asp), d-α-Asp, and d-β-Asp have been detected in proteins and peptides such as α-crystallin in the lens and β-amyloid in the brain. These residues increase with age, and hence they are associated with age-related diseases. The enzyme protein d-aspartyl (l-isoaspartyl) O-methyltransferase (PIMT) can revert these residues back to the normal l-α-Asp residue. PIMT catalyzes transmethylation of S-adenosylmethionine to l-β-Asp and d-α-Asp residues in proteins and peptides. In this work, the substrate recognition mechanism of PIMT was investigated using docking and molecular dynamics simulation studies. It was shown that the hydrogen bonds of Ser60...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Ikuhiko, Noji , Akifumi, Oda , Kana, Kobayashi , Ohgi, Takahashi
Unusual amino acid residues such as l-β-aspartyl (Asp), d-α-Asp, and d-β-Asp have been detected in proteins and peptides such as α-crystallin in the lens and β-amyloid in the brain. These residues increase with age, and hence they are associated with age-related diseases. The enzyme protein d-aspartyl (l-isoaspartyl) O-methyltransferase (PIMT) can revert these residues back to the normal l-α-Asp residue. PIMT catalyzes transmethylation of S-adenosylmethionine to l-β-Asp and d-α-Asp residues in proteins and peptides. In this work, the substrate recognition mechanism of PIMT was investigated using docking and molecular dynamics simulation studies. It was shown that the hydrogen bonds of Ser60...
Application of hollow fiber liquid phase microextraction coupled with high–per -formance liquid chromatography for the study of the osthole pharmacokinetics in cerebral ischemia hypoperfusion rat plasma
21 June 2011, 21:42:29
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Jun, Zhou , Ping, Zeng , Zhao Hui, Cheng , Jing, Liu , Feng Qiao, Wang , ...
A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction has been developed and used to quantify the osthole in cerebral ischemia reperfusion rat plasma following oral administration. The analysis was performed by reversed phase high performance liquid chromatography with fluorescence detection. Extraction conditions such as solvent identity, agitation rate, salt concentration, extraction time, and length of the hollow fiber were optimized. Under the optimized conditions, the linear range of osthole in rat plasma was 5–500ngmL−1 (r2=0.9997). The limit of detection (LOD) was 2ngmL−1 (S/N=3) with limit of quantification (LOQ) being 5ngmL−1. The validated method...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Jun, Zhou , Ping, Zeng , Zhao Hui, Cheng , Jing, Liu , Feng Qiao, Wang , ...
A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction has been developed and used to quantify the osthole in cerebral ischemia reperfusion rat plasma following oral administration. The analysis was performed by reversed phase high performance liquid chromatography with fluorescence detection. Extraction conditions such as solvent identity, agitation rate, salt concentration, extraction time, and length of the hollow fiber were optimized. Under the optimized conditions, the linear range of osthole in rat plasma was 5–500ngmL−1 (r2=0.9997). The limit of detection (LOD) was 2ngmL−1 (S/N=3) with limit of quantification (LOQ) being 5ngmL−1. The validated method...
Determination of unbound ticagrelor and its active metabolite (AR-C124910XX) in human plasma by equilibrium dialysis and LC-MS/MS
21 June 2011, 21:42:29
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Henrik, Sillén , Melanie, Cook , Patty, Davis
Ticagrelor is the first direct acting reversibly binding oral platelet P2Y12 receptor antagonist. The parent molecule and the main metabolite (AR-C124910XX) are both able to block adenosine diphosphate-induced receptor signaling with similar potency. Drug binding to plasma proteins reduces free drug available for pharmacologic activity. Therefore, assessing unbound drug is important for interpretation of pharmacokinetic/pharmacodynamic findings. This paper describes the development and validation of an equilibrium dialysis/LC-MS/MS method for measuring unbound ticagrelor and AR-C124910XX in human plasma. Plasma samples (200μl) were dialysed against phosphate buffered saline (37°C, 24h) in 96-well dialysis plates to separate unbound analytes. Drug-protein binding alterations during...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Henrik, Sillén , Melanie, Cook , Patty, Davis
Ticagrelor is the first direct acting reversibly binding oral platelet P2Y12 receptor antagonist. The parent molecule and the main metabolite (AR-C124910XX) are both able to block adenosine diphosphate-induced receptor signaling with similar potency. Drug binding to plasma proteins reduces free drug available for pharmacologic activity. Therefore, assessing unbound drug is important for interpretation of pharmacokinetic/pharmacodynamic findings. This paper describes the development and validation of an equilibrium dialysis/LC-MS/MS method for measuring unbound ticagrelor and AR-C124910XX in human plasma. Plasma samples (200μl) were dialysed against phosphate buffered saline (37°C, 24h) in 96-well dialysis plates to separate unbound analytes. Drug-protein binding alterations during...
Therapeutic drug monitoring of tacrolimus by liquid chromatography-tandem mass spectrometry: Is it truly a routine test?
21 June 2011, 21:42:29
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Paul J., Taylor , Michael E. Franklin Chun-Hui, Tai , Peter I., Pillans
Therapeutic drug monitoring of tacrolimus by high-performance liquid chromatography-tandem mass spectrometry has become standard practice. We report on the long-term (4.5 years) use of one such method. Whole blood samples (25μL) were treated with zinc sulphate (100μL) and acetonitrile containing ascomycin (internal standard, 250μL). A high-performance liquid chromatography-tandem mass spectrometer operating in positive ion mode with an electrospray interface was used. Chromatography was performed on a TDM C18 cartridge column (10×2.1mm, 10μm, Waters) using a switch gradient. A total of 4029 batches were analyzed for tacrolimus; this comprised of 81950 analyses of which 61027 were patient samples. Calibration curves (1.0–50μg/L)...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 21 June 2011
Paul J., Taylor , Michael E. Franklin Chun-Hui, Tai , Peter I., Pillans
Therapeutic drug monitoring of tacrolimus by high-performance liquid chromatography-tandem mass spectrometry has become standard practice. We report on the long-term (4.5 years) use of one such method. Whole blood samples (25μL) were treated with zinc sulphate (100μL) and acetonitrile containing ascomycin (internal standard, 250μL). A high-performance liquid chromatography-tandem mass spectrometer operating in positive ion mode with an electrospray interface was used. Chromatography was performed on a TDM C18 cartridge column (10×2.1mm, 10μm, Waters) using a switch gradient. A total of 4029 batches were analyzed for tacrolimus; this comprised of 81950 analyses of which 61027 were patient samples. Calibration curves (1.0–50μg/L)...
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