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A paper from the latest issue:Mixture designs for exploring class diversity and metabolite fingerprinting: an efficient column chromatographic strategy.
09 July 2011, 21:35:36
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 8 July 2011
Daniely Xavier, Soares , Ieda S., Scarminio , Roy Edward, Bruns
The effects of four solvents, hexane, dichloromethane, ethyl acetate, methanol, and their mixtures on the separation of metabolites in crude extracts of Erythrina speciosa Andrews leaves were investigated using two strategies for open column chromatography. The classical extraction procedure was compared with mobile phases prepared according to a mixture design in order to explore the effects of solvent interactions on metabolite separations. Principal component analysis was used to compare the UV spectra obtained from RP-HPLC-DAD and to estimate the number of independent factors contained in the chromatographic data of the extracts. The results showed that, in addition to solvent polarity,...
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 8 July 2011
Daniely Xavier, Soares , Ieda S., Scarminio , Roy Edward, Bruns
The effects of four solvents, hexane, dichloromethane, ethyl acetate, methanol, and their mixtures on the separation of metabolites in crude extracts of Erythrina speciosa Andrews leaves were investigated using two strategies for open column chromatography. The classical extraction procedure was compared with mobile phases prepared according to a mixture design in order to explore the effects of solvent interactions on metabolite separations. Principal component analysis was used to compare the UV spectra obtained from RP-HPLC-DAD and to estimate the number of independent factors contained in the chromatographic data of the extracts. The results showed that, in addition to solvent polarity,...
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