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A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Rapid UHPLC determination of polyphenols in aqueous infusions of Salvia officinalis L. (sage tea)
04 July 2011, 21:39:20
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 July 2011
Benno F., Zimmermann , Stephan G., Walch , Laura Ngaba, Tinzoh , Wolf, Stühlinger , Dirk W., Lachenmeier
Sage tea, the aqueous infusion of dried sage leaves (Salvia officinalis L.), is used as a form of food as well as a form of traditional herbal medicine. Several in vivo and in vitro studies point to sage polyphenols as active principles that may inhibit lipid peroxidation and improve antioxidant defences. This study describes an UHPLC methodology with MS/MS and UV detection, which allows the separation, identification and quantification of the major phenolic constituents in sage tea within 34min, and was used to characterize 16 commercial brands of sage tea. The quantitatively dominating compounds were either rosmarinic acid (12.2 to...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 July 2011
Benno F., Zimmermann , Stephan G., Walch , Laura Ngaba, Tinzoh , Wolf, Stühlinger , Dirk W., Lachenmeier
Sage tea, the aqueous infusion of dried sage leaves (Salvia officinalis L.), is used as a form of food as well as a form of traditional herbal medicine. Several in vivo and in vitro studies point to sage polyphenols as active principles that may inhibit lipid peroxidation and improve antioxidant defences. This study describes an UHPLC methodology with MS/MS and UV detection, which allows the separation, identification and quantification of the major phenolic constituents in sage tea within 34min, and was used to characterize 16 commercial brands of sage tea. The quantitatively dominating compounds were either rosmarinic acid (12.2 to...
Development and validation of a sensitive LC/MS/MS method for the simultaneous determination of naloxone and its metabolites in mouse plasma
04 July 2011, 21:39:20
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 July 2011
Hongliang, Jiang , YuRong, Wang , Manjunath S., Shet , Yang, Zhang , Duane, Zenke , ...
A rapid, specific, and reliable LC-MS/MS based bioanalytical method was developed and validated for the simultaneous determination of naloxone (NLX) and its two metabolites, 6β-naloxol (NLL) and naloxone-3β-D-glucuronide (NLG) in mouse plasma. The optimal chromatographic behavior of these analytes was achieved on an Aquasil C18 column (50mm×2.1mm, 5μm) using reversed phase chromatography. The total LC analysis time per injection was 2.5minutes with a flow rate of 1.0mL/min with gradient elution. Sample preparation via protein precipitation with acetonitrile in a 96-well format was applied for analyses of these analytes. The analytes were monitored by electrospray ionization in positive ion multiple reaction...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 July 2011
Hongliang, Jiang , YuRong, Wang , Manjunath S., Shet , Yang, Zhang , Duane, Zenke , ...
A rapid, specific, and reliable LC-MS/MS based bioanalytical method was developed and validated for the simultaneous determination of naloxone (NLX) and its two metabolites, 6β-naloxol (NLL) and naloxone-3β-D-glucuronide (NLG) in mouse plasma. The optimal chromatographic behavior of these analytes was achieved on an Aquasil C18 column (50mm×2.1mm, 5μm) using reversed phase chromatography. The total LC analysis time per injection was 2.5minutes with a flow rate of 1.0mL/min with gradient elution. Sample preparation via protein precipitation with acetonitrile in a 96-well format was applied for analyses of these analytes. The analytes were monitored by electrospray ionization in positive ion multiple reaction...
On-line coupling of dynamic microwave-assisted extraction with high-speed counter-current chromatography for continuous isolation of nevadensin from Lyeicnotus pauciflorus Maxim
04 July 2011, 21:39:20
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
Xing, Tong , Xiaohua, Xiao , Gongke, Li
An on-line method based upon dynamic microwave-assisted extraction (DMAE) coupled with high-speed counter-current chromatography (HSCCC) was developed for continuous isolation of nevadensin from Lyeicnotus pauciflorus Maxim. The DMAE parameters were optimized by means of the Box-Behnken design. The maximum extraction yield was achieved using 30:1ml/g of liquid-solid ratio, 10ml/min of solvent flow rate and 200W of microwave power. The crude extracts were then separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (7:3:5:5, v/v/v/v). 13.0mg of nevadensin was isolated from 15.0g original sample by HSCCC with five times sample injection in 12h, and the isolation yield of...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
Xing, Tong , Xiaohua, Xiao , Gongke, Li
An on-line method based upon dynamic microwave-assisted extraction (DMAE) coupled with high-speed counter-current chromatography (HSCCC) was developed for continuous isolation of nevadensin from Lyeicnotus pauciflorus Maxim. The DMAE parameters were optimized by means of the Box-Behnken design. The maximum extraction yield was achieved using 30:1ml/g of liquid-solid ratio, 10ml/min of solvent flow rate and 200W of microwave power. The crude extracts were then separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (7:3:5:5, v/v/v/v). 13.0mg of nevadensin was isolated from 15.0g original sample by HSCCC with five times sample injection in 12h, and the isolation yield of...
QUANTITATIVE DETERMINATION OF T-2 TOXIN, HT-2 TOXIN, DEOXYNIVALENOL AND DEEPOXY-DEOXYNIVALENOL IN ANIMAL BODY FLUIDS USING LC-MS/MS DETECTION
04 July 2011, 21:39:20
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
S., De Baere , J., Goossens , A., Osselaere , M., Devreese , V., Vandenbroucke , ...
A sensitive and specific method for the quantitative determination of deoxynivalenol (DON), deepoxy-deoxynivalenol (DOM-1), T-2 toxin (T-2) and HT-2 toxin (HT-2) in animal body fluids (plasma and bile) using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is presented.The extraction of plasma consisted of a deproteinization step using methanol, followed by a clean-up using an Oasis® HLB solid-phase extraction column. For bile analysis, an extraction using a methanol/water mixture (70/30, v/v), followed by a liquid-liquid extraction using ethyl acetate, was performed.Chromatographic separation was achieved on a reversed-phase Nucleosil (100-5 C18 G100×3.0mm) column. For the analysis of DON and...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
S., De Baere , J., Goossens , A., Osselaere , M., Devreese , V., Vandenbroucke , ...
A sensitive and specific method for the quantitative determination of deoxynivalenol (DON), deepoxy-deoxynivalenol (DOM-1), T-2 toxin (T-2) and HT-2 toxin (HT-2) in animal body fluids (plasma and bile) using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is presented.The extraction of plasma consisted of a deproteinization step using methanol, followed by a clean-up using an Oasis® HLB solid-phase extraction column. For bile analysis, an extraction using a methanol/water mixture (70/30, v/v), followed by a liquid-liquid extraction using ethyl acetate, was performed.Chromatographic separation was achieved on a reversed-phase Nucleosil (100-5 C18 G100×3.0mm) column. For the analysis of DON and...
Simultaneous Determination of Fifteen Illegal Dyes in Animal Feeds and Poultry Products by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
04 July 2011, 21:39:20
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
Rongyuan, Liu , Wenjing, Hei , Pingli, He , Zhen, Li
With the increasing presence of illegal dyes, such as sudan reds and malachite green, in animal feeds and food products duirng the last few years, there is an urgent need of accurate quantitative determination methods for these illicit compounds. Here we established an accurate method for the simultaneous determination of 15 illegal dyes in animal feeds, meat, eggs and other food products using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The samples were extracted with a simple procedure using acetonitrile and solid phase extraction cleaning up. The application of C18 rapid column can achieve satisfactory separation of the 15...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 July 2011
Rongyuan, Liu , Wenjing, Hei , Pingli, He , Zhen, Li
With the increasing presence of illegal dyes, such as sudan reds and malachite green, in animal feeds and food products duirng the last few years, there is an urgent need of accurate quantitative determination methods for these illicit compounds. Here we established an accurate method for the simultaneous determination of 15 illegal dyes in animal feeds, meat, eggs and other food products using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The samples were extracted with a simple procedure using acetonitrile and solid phase extraction cleaning up. The application of C18 rapid column can achieve satisfactory separation of the 15...
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