A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from the latest issue:Development and validation of an EI-GC/MS method for the determination of sertraline and its major metabolite desmethyl-sertraline in blood
21 July 2011, 21:49:43
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Alaa, Khraiwesh , Ioannis, Papoutsis , Panagiota, Nikolaou , Constantinos, Pistos , Chara, Spiliopoulou , ...
Α sensitive and specific GC/MS method for the determination of sertraline and its main metabolite desmethyl-sertraline in whole blood has been developed, optimized and validated. Sample preparation included solid-phase extraction of both analytes and their derivatization with heptafluorobutyric anhydride (HFBA). Protriptyline was used as internal standard for the determination of both analytes. Limits of detection and quantification for both sertraline and desmethyl-sertraline were 0.30 and 1.00μg/L, respectively. The calibration curves were linear within the dynamic range of each analyte (1.00-500.0μg/L) with a correlation coefficient (R2) exceeding 0.991. Extraction efficiency ranged from 90.1(±5.8) to 95.4(±3.0) % for sertraline, and from 84.9(±8.2)...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Alaa, Khraiwesh , Ioannis, Papoutsis , Panagiota, Nikolaou , Constantinos, Pistos , Chara, Spiliopoulou , ...
Α sensitive and specific GC/MS method for the determination of sertraline and its main metabolite desmethyl-sertraline in whole blood has been developed, optimized and validated. Sample preparation included solid-phase extraction of both analytes and their derivatization with heptafluorobutyric anhydride (HFBA). Protriptyline was used as internal standard for the determination of both analytes. Limits of detection and quantification for both sertraline and desmethyl-sertraline were 0.30 and 1.00μg/L, respectively. The calibration curves were linear within the dynamic range of each analyte (1.00-500.0μg/L) with a correlation coefficient (R2) exceeding 0.991. Extraction efficiency ranged from 90.1(±5.8) to 95.4(±3.0) % for sertraline, and from 84.9(±8.2)...
The Effects of Hitchhiker Antigens Co-Eluting with Affinity-Purified Research Antibodies
21 July 2011, 21:49:43
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Lilly, Mechetner , Radhika, Sood , Van, Nguyen , Pete, Gagnon , Missag H., Parseghian
The popularity of Protein G for the purification of antibodies has given rise to an entire industry that supplies scientists with research grade immunoreagents; however, many times the supplied product is contaminated with antigens bound to the antibody's complementarity-determining regions (CDRs). These “hitchhikers” are a category of host cell proteins that are elusive to detect due to their interaction with the antibody in the final product and yet their impact on an experiment or an entire field of study can be far reaching. In an earlier work, the role of hitchhikers on a human anti-histone antibody destined for clinical usage...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Lilly, Mechetner , Radhika, Sood , Van, Nguyen , Pete, Gagnon , Missag H., Parseghian
The popularity of Protein G for the purification of antibodies has given rise to an entire industry that supplies scientists with research grade immunoreagents; however, many times the supplied product is contaminated with antigens bound to the antibody's complementarity-determining regions (CDRs). These “hitchhikers” are a category of host cell proteins that are elusive to detect due to their interaction with the antibody in the final product and yet their impact on an experiment or an entire field of study can be far reaching. In an earlier work, the role of hitchhikers on a human anti-histone antibody destined for clinical usage...
Self-assembly molecularly imprinted polymers of 17β-Estradiol on the surface of magnetic nanoparticles for selective separation and detection of estrogenic hormones in feeds
21 July 2011, 21:49:43
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Shu, Wang , Yun, Li , Meijuan, Ding , Xiaoli, Wu , Jinhui, Xu , ...
This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17β-Estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
Shu, Wang , Yun, Li , Meijuan, Ding , Xiaoli, Wu , Jinhui, Xu , ...
This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17β-Estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation...
COMPARATIVE STUDY OF LC-MS/MS AND UPLC-MS/MS FOR MULTI-RESIDUE ANALYSIS OF QUINOLONES, PENICILLINS AND CEPHALOSPORINS IN COW MILK, AND VALIDATION ACCORDING TO THE REGULATION 2002/657/EC
21 July 2011, 21:49:43
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
A., Junza , R., Amatya , D., Barrón , J., Barbosa
The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated β-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya,...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 20 July 2011
A., Junza , R., Amatya , D., Barrón , J., Barbosa
The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated β-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya,...
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