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Selected papers from the latest issue:A one-step electrochemical method for DNA detection that utilizes a peroxidase-mimicking DNAzyme amplified through PCR of target DNA
30 August 2011, 20:43:27
Publication year: 2011
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 30 August 2011
Byoung Yeon, Won , Sujeong, Shin , Rongzhan, Fu , Sung Chul, Shin , Dae-Yeon, Cho , ...
A novel one-step electrochemical method for DNA detection is described. The procedure utilizes a reaction catalyzed by a peroxidase-mimicking DNAzyme to produce a product, which forms an insoluble precipitation layer on the surface of an electrode. A rationally designed forward primer, conjugated with a peroxidase DNAzyme complementary sequence at its 5′-end, is used for PCR amplification of target DNA. As a result, the DNAzyme sequence is produced by amplification only when the target DNA is present in the sample. The PCR product is then subjected to the precipitation reaction on the electrode surface using an electrolyte assay buffer containing 4-chloronaphthol,...
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 30 August 2011
Byoung Yeon, Won , Sujeong, Shin , Rongzhan, Fu , Sung Chul, Shin , Dae-Yeon, Cho , ...
A novel one-step electrochemical method for DNA detection is described. The procedure utilizes a reaction catalyzed by a peroxidase-mimicking DNAzyme to produce a product, which forms an insoluble precipitation layer on the surface of an electrode. A rationally designed forward primer, conjugated with a peroxidase DNAzyme complementary sequence at its 5′-end, is used for PCR amplification of target DNA. As a result, the DNAzyme sequence is produced by amplification only when the target DNA is present in the sample. The PCR product is then subjected to the precipitation reaction on the electrode surface using an electrolyte assay buffer containing 4-chloronaphthol,...
Effects of sulfide on microbial fuel cells with platinum and nitrogen-doped carbon powder cathodes
30 August 2011, 20:43:27
Publication year: 2011
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 30 August 2011
Yujie, Feng , Xinxin, Shi , Xin, Wang , He, Lee , Jia, Liu , ...
Because of the advantages of low cost, good electrical conductivity and high oxidation resistance, nitrogen-doped carbon (NDC) materials have a potential to replace noble metals in microbial fuel cells (MFCs) for wastewater treatment. In spite of a large volume of studies on NDC materials as catalysts for oxygen reduction reaction, the influence of sulfide on NDC materials has not yet been explicitly reported so far. In this communication, nitrogen-doped carbon powders (NDCP) were prepared by treating carbon powders in nitric acid under reflux condition. Sodium sulfide (Na2S) was added to the cathodic electrolyte to compare its effects on platinum (Pt)...
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 30 August 2011
Yujie, Feng , Xinxin, Shi , Xin, Wang , He, Lee , Jia, Liu , ...
Because of the advantages of low cost, good electrical conductivity and high oxidation resistance, nitrogen-doped carbon (NDC) materials have a potential to replace noble metals in microbial fuel cells (MFCs) for wastewater treatment. In spite of a large volume of studies on NDC materials as catalysts for oxygen reduction reaction, the influence of sulfide on NDC materials has not yet been explicitly reported so far. In this communication, nitrogen-doped carbon powders (NDCP) were prepared by treating carbon powders in nitric acid under reflux condition. Sodium sulfide (Na2S) was added to the cathodic electrolyte to compare its effects on platinum (Pt)...
A multi-amplification aptasensor for highly sensitive detection of thrombin based on high-quality hollow CoPt nanoparticles decorated graphene
28 August 2011, 20:42:38
Publication year: 2011
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 27 August 2011
Yan, Wang , Ruo, Yuan , Yaqin, Chai , Yali, Yuan , Lijuan, Bai , ...
In this work, we have successfully demonstrated a facile strategy to incorporate high-quality hollow CoPt bimetal alloy nanoparticles (HCoPt) onto reduced graphene oxide sheet (HCoPt-RGs). An advanced sandwich-type electrochemical aptasensor for thrombin was proposed by using the HCoPt-RGs conjugates as secondary label. The formed conjugates provided large surface area for loading plentiful redox probe thionine (Thi), horseradish peroxidase (HRP) and secondary aptamer (Apt II) with good stability and friendly biocompatibility, indicating its superior properties in electroactive mediater enrichment and biomolecule immobilization. Furthermore, activated by glutaraldehyde (GA), the chitosan-hollow CoPt alloy nanoparticles (CS-HCoPt) film can greatly facilitate the capture of primary...
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 27 August 2011
Yan, Wang , Ruo, Yuan , Yaqin, Chai , Yali, Yuan , Lijuan, Bai , ...
In this work, we have successfully demonstrated a facile strategy to incorporate high-quality hollow CoPt bimetal alloy nanoparticles (HCoPt) onto reduced graphene oxide sheet (HCoPt-RGs). An advanced sandwich-type electrochemical aptasensor for thrombin was proposed by using the HCoPt-RGs conjugates as secondary label. The formed conjugates provided large surface area for loading plentiful redox probe thionine (Thi), horseradish peroxidase (HRP) and secondary aptamer (Apt II) with good stability and friendly biocompatibility, indicating its superior properties in electroactive mediater enrichment and biomolecule immobilization. Furthermore, activated by glutaraldehyde (GA), the chitosan-hollow CoPt alloy nanoparticles (CS-HCoPt) film can greatly facilitate the capture of primary...
Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria
28 August 2011, 20:42:38
Publication year: 2011
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 27 August 2011
Jin, Zhou , ThetNaing, Tun , Sung-ha, Hong , June D., Mercer-Chalmers , Maisem, Laabei , ...
A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxy-fluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic E. coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin)and lipases(phospholipase A2). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S....
Source: Biosensors and Bioelectronics, In Press, Accepted Manuscript, Available online 27 August 2011
Jin, Zhou , ThetNaing, Tun , Sung-ha, Hong , June D., Mercer-Chalmers , Maisem, Laabei , ...
A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxy-fluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic E. coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin)and lipases(phospholipase A2). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S....
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