A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from this issue:Enantioselective determination of 3-hydroxybutyrate in the tissues of normal and streptozotocin-induced diabetic rats of different ages
04 August 2011, 21:46:32
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 4 August 2011
Wei-Yu, Hsu , Chen-Yi, Kuo , Takeshi, Fukushima , Kazuhiro, Imai , Chien-Ming, Chen , ...
l-3-hydroxybutyrate (3HB) and d-3HB are enantiomers that exist in various rat tissues, and the ratio of the 2 compounds is of importance since it may affect glucose utilization in cardiomyocytes. In this study, we determined the concentrations of l-3HB and d-3HB in the tissues of normal and streptozotocin (STZ)-induced diabetic rats of different ages by column-switching high-performance liquid chromatography using a fluorescence detection system. In normal rats, the levels of l-3HB peaked at 8 weeks of age in the cerebrum, liver, spleen, lung, kidney, adrenal gland, and heart and then decreased afterwards. The concentrations of l-3HB were the highest in...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 4 August 2011
Wei-Yu, Hsu , Chen-Yi, Kuo , Takeshi, Fukushima , Kazuhiro, Imai , Chien-Ming, Chen , ...
l-3-hydroxybutyrate (3HB) and d-3HB are enantiomers that exist in various rat tissues, and the ratio of the 2 compounds is of importance since it may affect glucose utilization in cardiomyocytes. In this study, we determined the concentrations of l-3HB and d-3HB in the tissues of normal and streptozotocin (STZ)-induced diabetic rats of different ages by column-switching high-performance liquid chromatography using a fluorescence detection system. In normal rats, the levels of l-3HB peaked at 8 weeks of age in the cerebrum, liver, spleen, lung, kidney, adrenal gland, and heart and then decreased afterwards. The concentrations of l-3HB were the highest in...
A High-Throughput U-HPLC-MS/MS Assay for the Quantification of Mycophenolic Acid and its Major Metabolites Mycophenolic Acid Glucuronide and Mycophenolic Acid Acyl-Glucuronide in Human Plasma and Urine.
02 August 2011, 21:45:36
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Jacek, Klepacki , Jelena, Klawitter , Jamie, Bendrick-Peart , Bjoern, Schniedewind , Svenja, Heischmann , ...
Mycophenolic acid (MPA) is used as an immunosuppressant after organ transplantation and for the treatment of immune diseases. There is increasing evidence that therapeutic drug monitoring and plasma concentration-guided dose adjustments are beneficial for patients to maintain immunosuppressive efficacy and to avoid toxicity. The major MPA metabolite that can be found in high concentrations in plasma is MPA glucuronide (MPAG). A metabolite usually present at lower concentrations, MPA acyl-glucuronide (AcMPAG), has been implicated in some of the adverse effects of MPA.We developed and validated an automated high-throughput ultra-high performance chromatography-tandem mass spectrometry (U-HPLC-MS/MS) assay using liquid-handling robotic extraction for the...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Jacek, Klepacki , Jelena, Klawitter , Jamie, Bendrick-Peart , Bjoern, Schniedewind , Svenja, Heischmann , ...
Mycophenolic acid (MPA) is used as an immunosuppressant after organ transplantation and for the treatment of immune diseases. There is increasing evidence that therapeutic drug monitoring and plasma concentration-guided dose adjustments are beneficial for patients to maintain immunosuppressive efficacy and to avoid toxicity. The major MPA metabolite that can be found in high concentrations in plasma is MPA glucuronide (MPAG). A metabolite usually present at lower concentrations, MPA acyl-glucuronide (AcMPAG), has been implicated in some of the adverse effects of MPA.We developed and validated an automated high-throughput ultra-high performance chromatography-tandem mass spectrometry (U-HPLC-MS/MS) assay using liquid-handling robotic extraction for the...
Liquid chromatography–mass spectrometry method for the quantification of tamoxifen and its metabolite 4-hydroxytamoxifen in rat plasma: Application to interaction study with biochanin A (an isoflavone)
02 August 2011, 21:45:36
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Sheelendra Pratap, Singh , Wahajuddin , Mushir M., Ali , Kanchan, Kohli , Girish Kumar, Jain
Tamoxifen is the agent of choice for the treatment of estrogen receptor-positive breast cancer. Tamoxifen is a substrate of P-glycoprotein (P-gp) and microsomal cytochrome P450 (CYP) 3A, and biochanin A (BCA) is an inhibitor of P-gp and CYP3A. Hence, it could be expected that BCA would affect the pharmacokinetics of tamoxifen. In the present study we have developed and validated a simple, sensitive and specific LC-ESI-MS/MS method for the simultaneous quantification of tamoxifen and its metabolite 4-hydroxytamoxifen with 100μL rat plasma using centchroman as an internal standard (IS). Tamoxifen, 4-hydroxytamoxifen and IS were separated on a Supelco Discovery C18 (4.6×50mm,...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Sheelendra Pratap, Singh , Wahajuddin , Mushir M., Ali , Kanchan, Kohli , Girish Kumar, Jain
Tamoxifen is the agent of choice for the treatment of estrogen receptor-positive breast cancer. Tamoxifen is a substrate of P-glycoprotein (P-gp) and microsomal cytochrome P450 (CYP) 3A, and biochanin A (BCA) is an inhibitor of P-gp and CYP3A. Hence, it could be expected that BCA would affect the pharmacokinetics of tamoxifen. In the present study we have developed and validated a simple, sensitive and specific LC-ESI-MS/MS method for the simultaneous quantification of tamoxifen and its metabolite 4-hydroxytamoxifen with 100μL rat plasma using centchroman as an internal standard (IS). Tamoxifen, 4-hydroxytamoxifen and IS were separated on a Supelco Discovery C18 (4.6×50mm,...
A quantitative LC-MS/MS method for comparative analysis of capture-antibody affinity toward protein antigens
02 August 2011, 21:45:36
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Mark S., Lowenthal , Hugo, Gasca-Aragon , John E., Schiel , Nathan G., Dodder , David M., Bunk
A mass spectrometry-based antibody selection procedure was developed to evaluate optimal ‘capture’ monoclonal antibodies that can be used in a variety of analytical measurement applications. The isotope-dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS) methodology is based on the use of multiple-reaction monitoring of tryptic peptide fragments derived from protein antigens. A panel of monoclonal antibodies (mAb) was evaluated based on a quantitative determination of relative binding affinity to human cardiac troponin I following immunoprecipitation. Dissociation constants (Kd) were determined for ‘bound mAb-antigen’ vs. ‘unbound antigen’ using non-linear regression analysis. Relative quantification of both antigen and antibody was based on the...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 2 August 2011
Mark S., Lowenthal , Hugo, Gasca-Aragon , John E., Schiel , Nathan G., Dodder , David M., Bunk
A mass spectrometry-based antibody selection procedure was developed to evaluate optimal ‘capture’ monoclonal antibodies that can be used in a variety of analytical measurement applications. The isotope-dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS) methodology is based on the use of multiple-reaction monitoring of tryptic peptide fragments derived from protein antigens. A panel of monoclonal antibodies (mAb) was evaluated based on a quantitative determination of relative binding affinity to human cardiac troponin I following immunoprecipitation. Dissociation constants (Kd) were determined for ‘bound mAb-antigen’ vs. ‘unbound antigen’ using non-linear regression analysis. Relative quantification of both antigen and antibody was based on the...
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