A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from the latest issue:Advancing liquid/liquid extraction through a novel microfluidic device: theory, instrumentation and applications in gas chromatography
07 August 2011, 20:45:50
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 7 August 2011
Daniela, Peroni , Wil, van Egmond , Wim Th., Kok , Hans-Gerd, Janssen
A new chip-based liquid-liquid extraction technique for sample preparation of aqueous samples for GC was developed. Extraction is performed in a segmented flow system with additional mixing provided by an etched channel structure. The dimensions of the device are optimized to allow benefiting of the advantages of chip technology without suffering from the limitations of over-miniaturization. Phase separation is performed with a novel phase separator developed in house. The instrumental set-up is simple. The results obtained for selected test analytes show that the extraction is quantitative (recoveries=92-110%, RSD
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 7 August 2011
Daniela, Peroni , Wil, van Egmond , Wim Th., Kok , Hans-Gerd, Janssen
A new chip-based liquid-liquid extraction technique for sample preparation of aqueous samples for GC was developed. Extraction is performed in a segmented flow system with additional mixing provided by an etched channel structure. The dimensions of the device are optimized to allow benefiting of the advantages of chip technology without suffering from the limitations of over-miniaturization. Phase separation is performed with a novel phase separator developed in house. The instrumental set-up is simple. The results obtained for selected test analytes show that the extraction is quantitative (recoveries=92-110%, RSD
Separation of Exenatide analogue mono-PEGylated with 40 kDA polyethylene glycol by cation exchange chromatography
07 August 2011, 20:45:50
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 7 August 2011
Cai, Yongqing , Yue, Peng
Random PEGylation usually resulted in product mixtures composed of mono-PEGylated isomers and multie-PEGylated attachments. Generally in PEGylation research, separation of the mono-PEGylated isomers was the prerequisite for finding the optimal PEGylation site. However, when peptides or proteins were PEGylated with polyethylene glycol as large as 40 kDA, the physicochemical properties like hydrophobicity and molecular size of the isomers would become too similar to make the routine separation methods, like RP-HPLC, size-exclusion chromatography, SDS-PAGE and capillary isoelectric focusing invalid. This article presented a useful method of successfully separating exenatide analogue (an incretin for diabetic therapy) isomers mono-PEGylated with 40 kDA polyethylene...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 7 August 2011
Cai, Yongqing , Yue, Peng
Random PEGylation usually resulted in product mixtures composed of mono-PEGylated isomers and multie-PEGylated attachments. Generally in PEGylation research, separation of the mono-PEGylated isomers was the prerequisite for finding the optimal PEGylation site. However, when peptides or proteins were PEGylated with polyethylene glycol as large as 40 kDA, the physicochemical properties like hydrophobicity and molecular size of the isomers would become too similar to make the routine separation methods, like RP-HPLC, size-exclusion chromatography, SDS-PAGE and capillary isoelectric focusing invalid. This article presented a useful method of successfully separating exenatide analogue (an incretin for diabetic therapy) isomers mono-PEGylated with 40 kDA polyethylene...
Tools to discover anionic and nonionic polyfluorinated alkyl surfactants by liquid chromatography electrospray ionisation mass spectrometry
07 August 2011, 20:45:50
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Xenia, Trier , Kit, Granby , Jan H., Christensen
A tiered approach is proposed for the discovery of unknown anionic and nonionic polyfluorinated alkyl surfactants (PFASs) by reversed phase ultra performance liquid chromatography - negative electrospray ionisation - quadrupole time of flight mass spectrometry (UPLC-ESI--QTOF-MS). The chromatographic separation, ionisation and detection of PFASs mixtures, was achieved at high pH (pH=9.7) with NH4OH as additive. To distinguish PFASs from other chemicals we used the characteristic negative mass defects of PFASs, their specific losses of 20Da (HF) and the presence of series of chromatographic peaks, belonging to homologues series with m/z of n×50Da (CF2) or n×100Da (CF2CF2). The elemental composition of...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Xenia, Trier , Kit, Granby , Jan H., Christensen
A tiered approach is proposed for the discovery of unknown anionic and nonionic polyfluorinated alkyl surfactants (PFASs) by reversed phase ultra performance liquid chromatography - negative electrospray ionisation - quadrupole time of flight mass spectrometry (UPLC-ESI--QTOF-MS). The chromatographic separation, ionisation and detection of PFASs mixtures, was achieved at high pH (pH=9.7) with NH4OH as additive. To distinguish PFASs from other chemicals we used the characteristic negative mass defects of PFASs, their specific losses of 20Da (HF) and the presence of series of chromatographic peaks, belonging to homologues series with m/z of n×50Da (CF2) or n×100Da (CF2CF2). The elemental composition of...
Effect of chromatographic conditions on liquid chromatographic chiral separation of terbutaline and salbutamol on Chirobiotic V column
07 August 2011, 20:45:50
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Hisham, Hashem , Clemens, Tründelberg , Omer, Attef , Thomas, Jira
In this study, a method for enantioseparation of terbutaline and salbutamol was established using Chirobiotic V column as a stationary phase. Polar ionic mode applying mobile phase containing ammonium nitrate in 100% ethanol, pH 5.1 was found to give the best separation. The salt concentration in the mobile phase and pH value were found to be the most important chromatographic factors affecting separation. Separation of enantiomers of these two basic analytes was complete in less than 10minutes without applying ammonium trifluoroacetate (ATFA) or triethylamine (TEA) salts.
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Hisham, Hashem , Clemens, Tründelberg , Omer, Attef , Thomas, Jira
In this study, a method for enantioseparation of terbutaline and salbutamol was established using Chirobiotic V column as a stationary phase. Polar ionic mode applying mobile phase containing ammonium nitrate in 100% ethanol, pH 5.1 was found to give the best separation. The salt concentration in the mobile phase and pH value were found to be the most important chromatographic factors affecting separation. Separation of enantiomers of these two basic analytes was complete in less than 10minutes without applying ammonium trifluoroacetate (ATFA) or triethylamine (TEA) salts.
Selective enrichment of the degradation products of organophosphorus nerve agents by zirconia based solid-phase extraction
07 August 2011, 20:45:50
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Pankaj K., Kanaujia , Deepak, Pardasani , Vijay, Tak , Ajay K., Purohit , D.K., Dubey
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX. Critical extraction parameters such as modifier concentration, nature and volume of washing and eluting solvents were investigated. Amongst other anionic compounds, selectivity in extraction was observed for organophosphorus compounds. Recoveries of analytes were determined by GC-MS which ranged from 80 to 115%. Comparison of zirconia based solid-phase extraction method with anion-exchange solid-phase extraction revealed its selectivity towards phosphonic acids. The...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 6 August 2011
Pankaj K., Kanaujia , Deepak, Pardasani , Vijay, Tak , Ajay K., Purohit , D.K., Dubey
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX. Critical extraction parameters such as modifier concentration, nature and volume of washing and eluting solvents were investigated. Amongst other anionic compounds, selectivity in extraction was observed for organophosphorus compounds. Recoveries of analytes were determined by GC-MS which ranged from 80 to 115%. Comparison of zirconia based solid-phase extraction method with anion-exchange solid-phase extraction revealed its selectivity towards phosphonic acids. The...
No comments:
Post a Comment