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Selected papers from the latest issue:Cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography for analysis of methadone and its metabolites in serum of heroin addicts
10 August 2011, 20:46:44
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Chun-Chi, Wang , Cheng-Chung, Chen , Shang-Jang, Wang , Shou-Mei, Wu
Methadone (MET) metabolism has been largely demonstrated with a high inter-individual variability and, therefore, quantification of MET is very important for therapeutic drug monitoring. A cation-selective exhaustive injection and sweeping MEKC (CSEI-Sweeping) was first developed to analyze MET and its two metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP), in human serum. After pretreatment, the samples were electrokinetically injected into capillary (10kV, 500s) and swept by the separation phosphate buffer (100mM, pH 4.0) containing 20% tetrahydrofuran and 100mM SDS at -15kV. The LODs were 200pg/mL for MET and EMDP, and 400pg/mL for EDDP. Ten volunteers were administered MET (5.0 to 120.0mg/d) orally...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Chun-Chi, Wang , Cheng-Chung, Chen , Shang-Jang, Wang , Shou-Mei, Wu
Methadone (MET) metabolism has been largely demonstrated with a high inter-individual variability and, therefore, quantification of MET is very important for therapeutic drug monitoring. A cation-selective exhaustive injection and sweeping MEKC (CSEI-Sweeping) was first developed to analyze MET and its two metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP), in human serum. After pretreatment, the samples were electrokinetically injected into capillary (10kV, 500s) and swept by the separation phosphate buffer (100mM, pH 4.0) containing 20% tetrahydrofuran and 100mM SDS at -15kV. The LODs were 200pg/mL for MET and EMDP, and 400pg/mL for EDDP. Ten volunteers were administered MET (5.0 to 120.0mg/d) orally...
Cooperative Effect in Ion Pairing of Oligolysine with Heptafluorobutyric Acid in Reversed-Phase Chromatography
10 August 2011, 20:46:44
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Wenchun, Xie , Xu, Qin , Iwao, Teraoka , Richard A, Gross
The retention behavior of an oligolysine mixture, consisting of two to eight residues, was examined at different concentrations of heptafluorobutyric acid (HFBA) in the mobile phase using a C18 column. A single ion record (SIR) mode of the mass spectrometer produced a distinct retention time for each oligomer component. As the concentration of HFBA increased, the retention time of each oligomer increased. Furthermore, the increase in retention time is chain-length dependent such that, the longer the oligomer chain, the more rapid was the rate that retention time increased. A closed pairing model that presumes an equilibrium between the unpaired state...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Wenchun, Xie , Xu, Qin , Iwao, Teraoka , Richard A, Gross
The retention behavior of an oligolysine mixture, consisting of two to eight residues, was examined at different concentrations of heptafluorobutyric acid (HFBA) in the mobile phase using a C18 column. A single ion record (SIR) mode of the mass spectrometer produced a distinct retention time for each oligomer component. As the concentration of HFBA increased, the retention time of each oligomer increased. Furthermore, the increase in retention time is chain-length dependent such that, the longer the oligomer chain, the more rapid was the rate that retention time increased. A closed pairing model that presumes an equilibrium between the unpaired state...
Kinetic plot based comparison of the efficiency and peak capacity of high-performance liquid chromatography columns: theoretical background and selected examples
10 August 2011, 20:46:44
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Ken, Broeckhoven , Deirdre, Cabooter , Sebastiaan, Eeltink , Gert, Desmet
The present contribution reviews the foundations of the kinetic-plot method for the direct comparison of the kinetic performance of different chromatographic support and operating modes. The method directly uses experimental data collected for a specific sample and operating condition of one's interest, and is applicable both under isocratic- and gradient-elution conditions. Experimental proof is provided for the strong relation between the kinetic performance of a given support under isocratic and gradient conditions: a material offering superior kinetic performances under isocratic conditions will remain superior under gradient conditions and vice versa provided the comparison occurs under unbiased conditions. In addition, a...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
Ken, Broeckhoven , Deirdre, Cabooter , Sebastiaan, Eeltink , Gert, Desmet
The present contribution reviews the foundations of the kinetic-plot method for the direct comparison of the kinetic performance of different chromatographic support and operating modes. The method directly uses experimental data collected for a specific sample and operating condition of one's interest, and is applicable both under isocratic- and gradient-elution conditions. Experimental proof is provided for the strong relation between the kinetic performance of a given support under isocratic and gradient conditions: a material offering superior kinetic performances under isocratic conditions will remain superior under gradient conditions and vice versa provided the comparison occurs under unbiased conditions. In addition, a...
DETERMINATION OF CHLOROPROPANOLS IN FOODS BY ONE-STEP EXTRACTION AND DERIVATIZATION USING PRESSURIZED LIQUID EXTRACTION AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY
10 August 2011, 20:46:44
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
I., Racamonde , P., González , R.A., LOrenzo , A.M., Carro
3-chloropropane-1,2-diol (3-MCPD) and 1,3-dichloro-2-propanol (1,3-DCP) were determined for the first time in bakery foods using pressurized liquid extraction (PLE) combined with in-situ derivatization and GC–MS analysis. This one-step protocol uses N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) as silylation reagent. Initially, screening experimental design was applied to evaluate the effects of the variables potentially affecting the extraction process, namely extraction time (min) and temperature (°C), number of cycles, dispersant reagent (diatomaceous earth in powder form and as particulate matter with high pore volume Extrelut NT) and percent of flush ethyl acetate volume (%). To reduce the time of analysis and improve the sensitivity, derivatization of...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 10 August 2011
I., Racamonde , P., González , R.A., LOrenzo , A.M., Carro
3-chloropropane-1,2-diol (3-MCPD) and 1,3-dichloro-2-propanol (1,3-DCP) were determined for the first time in bakery foods using pressurized liquid extraction (PLE) combined with in-situ derivatization and GC–MS analysis. This one-step protocol uses N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) as silylation reagent. Initially, screening experimental design was applied to evaluate the effects of the variables potentially affecting the extraction process, namely extraction time (min) and temperature (°C), number of cycles, dispersant reagent (diatomaceous earth in powder form and as particulate matter with high pore volume Extrelut NT) and percent of flush ethyl acetate volume (%). To reduce the time of analysis and improve the sensitivity, derivatization of...
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