Protein adsorption and transport in dextran-modified ion-exchange media III. Effects of resin charge density and dextran content on adsorption and intraparticle uptake
20 August 2011, 20:47:15
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Brian D., Bowes , Abraham M., Lenhoff
Custom-synthesized variants of the commercial Capto S resin were used to examine the effects of resin charge density and dextran content on protein adsorption and intraparticle uptake. For the small protein lysozyme, resin charge density had the greatest effect on equilibrium capacity, consistent with calculations suggesting that lysozyme capacity should be limited by the available charge on the resin. Isocratic retention data and confocal microscopy imaging for this protein revealed a consistent ordering of the resins linking stronger protein–resin interactions with higher static capacities but slower intraparticle uptake rates over the range of properties studied. For the larger protein lactoferrin,...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Brian D., Bowes , Abraham M., Lenhoff
Custom-synthesized variants of the commercial Capto S resin were used to examine the effects of resin charge density and dextran content on protein adsorption and intraparticle uptake. For the small protein lysozyme, resin charge density had the greatest effect on equilibrium capacity, consistent with calculations suggesting that lysozyme capacity should be limited by the available charge on the resin. Isocratic retention data and confocal microscopy imaging for this protein revealed a consistent ordering of the resins linking stronger protein–resin interactions with higher static capacities but slower intraparticle uptake rates over the range of properties studied. For the larger protein lactoferrin,...
Effect of pH additive and column temperature on kinetic performance of two different sub-2μm stationary phases for ultrafast separation of charged analytes.
20 August 2011, 20:47:15
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Sabine, Heinisch , Amélie, D’Attoma , Candice, Grivel
The performance characteristics of separation were studied for small pharmaceuticals and larger charged molecules (peptides) in various mobile phase conditions on two 5-cm long narrow bore columns packed with 1.7μm core-shell and totally porous particles respectively. The effect of temperature and pH additives (formic acid, trifluoroacetic acid, ammonium formate, ammonium acetate and ammonia) on column efficiency was investigated through a kinetic study based upon data obtained under gradient elution conditions. Sample peak capacities were calculated and compared in all studied conditions for a sample of ten representative peptides having masses ranging from 500 to 2000Da. The elevation of temperature was...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Sabine, Heinisch , Amélie, D’Attoma , Candice, Grivel
The performance characteristics of separation were studied for small pharmaceuticals and larger charged molecules (peptides) in various mobile phase conditions on two 5-cm long narrow bore columns packed with 1.7μm core-shell and totally porous particles respectively. The effect of temperature and pH additives (formic acid, trifluoroacetic acid, ammonium formate, ammonium acetate and ammonia) on column efficiency was investigated through a kinetic study based upon data obtained under gradient elution conditions. Sample peak capacities were calculated and compared in all studied conditions for a sample of ten representative peptides having masses ranging from 500 to 2000Da. The elevation of temperature was...
Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples
20 August 2011, 20:47:15
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Ximei, Wu , Jun, Hu , Binghui, Zhu , Lin, Lu , Xiangdong, Huang , ...
A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5––50μg/kg yielded mean recoveries from 67 to 90%, respectively. Finally, this oligosorbent was applied...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Ximei, Wu , Jun, Hu , Binghui, Zhu , Lin, Lu , Xiangdong, Huang , ...
A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5––50μg/kg yielded mean recoveries from 67 to 90%, respectively. Finally, this oligosorbent was applied...
QUANTITATIVE TRACE ANALYSIS OF EIGHT CHLORAMPHENICOL ISOMERS IN URINE BY CHIRAL LIQUID CHROMATOGRAPHY COUPLED TO TANDEM MASS SPECTROMETRY
20 August 2011, 20:47:15
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Bjorn J.A., Berendsen , Martien L., Essers , Linda (A.)A.M., Stolker , Michel W.F., Nielen
Chloramphenicol is a broad-spectrum antibiotic with, apart from its human medicinal use, veterinary abuse in all major food-producing animals. Chloramphenicol occurs in four stereoisomers (all para-nitro substituted) and furthermore four meta-nitro analogs of chloramphenicol exist. In this paper these are reffered to as eight chloramphenicol isomers. According to EU regulations an analytical method should be able to discriminate the analyte from interfering substances that might be present in the sample, including isomers. For the first time a quantitative method for the analysis of trace levels of eight chloramphenicol isomers in urine by chiral liquid chromatography in combination with tandem mass...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Bjorn J.A., Berendsen , Martien L., Essers , Linda (A.)A.M., Stolker , Michel W.F., Nielen
Chloramphenicol is a broad-spectrum antibiotic with, apart from its human medicinal use, veterinary abuse in all major food-producing animals. Chloramphenicol occurs in four stereoisomers (all para-nitro substituted) and furthermore four meta-nitro analogs of chloramphenicol exist. In this paper these are reffered to as eight chloramphenicol isomers. According to EU regulations an analytical method should be able to discriminate the analyte from interfering substances that might be present in the sample, including isomers. For the first time a quantitative method for the analysis of trace levels of eight chloramphenicol isomers in urine by chiral liquid chromatography in combination with tandem mass...
Comprehensive analysis of Maté (Ilex paraguariensis) compounds: Development of chemical strategies for matesaponin analysis by mass spectrometry
20 August 2011, 20:47:15
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Lauro M., de Souza , Nessana, Dartora , Camila T., Scoparo , Thales R., Cipriani , Philip A.J., Gorin , ...
Ilex paraguariensis (maté) is an important plant from southern Brazil, Paraguay, Uruguay and Argentina, where its leaves are widely use in hot or cold beverages. We now present a comprehensive ULPC-PDA-MS analysis of the compounds found in its leaves. The analysis was rapid, less than 13min and many compounds were identified, among these, the chlorogenic acid series, and other quinic acid derivatives, xanthines, and several saponins, many of which have not been previously described, including many isomers. The isomerism could occur in the aglycone moiety, namely ursolic or oleanolic acid, or in the carbohydrate moiety. Additionally, many acetylated saponins were...
Highlights: ► a rapid and comprehensive liquid chromatography analysis of maté components was developed ► chemical derivatization was employed in order to aid the analysis of matesaponins by mass spectrometry ► several unreported matesaponins were described.
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 19 August 2011
Lauro M., de Souza , Nessana, Dartora , Camila T., Scoparo , Thales R., Cipriani , Philip A.J., Gorin , ...
Ilex paraguariensis (maté) is an important plant from southern Brazil, Paraguay, Uruguay and Argentina, where its leaves are widely use in hot or cold beverages. We now present a comprehensive ULPC-PDA-MS analysis of the compounds found in its leaves. The analysis was rapid, less than 13min and many compounds were identified, among these, the chlorogenic acid series, and other quinic acid derivatives, xanthines, and several saponins, many of which have not been previously described, including many isomers. The isomerism could occur in the aglycone moiety, namely ursolic or oleanolic acid, or in the carbohydrate moiety. Additionally, many acetylated saponins were...
Highlights: ► a rapid and comprehensive liquid chromatography analysis of maté components was developed ► chemical derivatization was employed in order to aid the analysis of matesaponins by mass spectrometry ► several unreported matesaponins were described.
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