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Selected papers from the latest issue:Comparison of monolithic and microparticulate columns for reversed-phase liquid chromatography of tryptic digests of industrial enzymes in cleaning products
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
M., Beneito-Cambra , J.M., Herrero-Martínez , G., Ramis-Ramos , W., Lindner , M., Lämmerhofer
Enzymes of several classes used in the formulations of cleaning products were characterized by trypsin digestion followed by HPLC with UV detection. A polymeric monolithic column (ProSwift) was used to optimize the separation of both the intact enzymes and their tryptic digests. This column was adequate for the quality control of raw industrial enzyme concentrates. Then, monolithic and microparticulate columns were compared for peptide analysis. Under optimized conditions, the analysis of tryptic digests of enzymes of different classes commonly used in the formulation of cleaning products was carried out. Number of peaks, peak capacity and global resolution were obtained in...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
M., Beneito-Cambra , J.M., Herrero-Martínez , G., Ramis-Ramos , W., Lindner , M., Lämmerhofer
Enzymes of several classes used in the formulations of cleaning products were characterized by trypsin digestion followed by HPLC with UV detection. A polymeric monolithic column (ProSwift) was used to optimize the separation of both the intact enzymes and their tryptic digests. This column was adequate for the quality control of raw industrial enzyme concentrates. Then, monolithic and microparticulate columns were compared for peptide analysis. Under optimized conditions, the analysis of tryptic digests of enzymes of different classes commonly used in the formulation of cleaning products was carried out. Number of peaks, peak capacity and global resolution were obtained in...
Structural identification of mouse fecal metabolites of theaflavin 3, 3′-digallate using liquid chromatography tandem mass spectrometry
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Huadong, Chen , Tiffany A., Parks , Xiaoxin, Chen , Nicholas D., Gillitt , Christian, Jobin , ...
Black tea consumption has been associated with many health benefits including the prevention of cancer and heart disease. Theaflavins are the major bioactive polyphenols present in black tea. Unfortunately, limited information is available on their biotransformation. In the present study, we investigated the metabolic fate of theaflavin 3, 3′-digallate (TFDG), one of the most abundant and bioactive theaflavins, in mouse fecal samples using liquid chromatography/electrospray ionization tandem mass spectrometry by analyzing the MSn (n=1–3) spectra. Four metabolites theaflavin, theaflavin 3-gallate, theaflavin 3′-gallate, and gallic acid were identified as the major mouse fecal metabolites of TFDG. Glucuronidated and sulfated, instead of...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Huadong, Chen , Tiffany A., Parks , Xiaoxin, Chen , Nicholas D., Gillitt , Christian, Jobin , ...
Black tea consumption has been associated with many health benefits including the prevention of cancer and heart disease. Theaflavins are the major bioactive polyphenols present in black tea. Unfortunately, limited information is available on their biotransformation. In the present study, we investigated the metabolic fate of theaflavin 3, 3′-digallate (TFDG), one of the most abundant and bioactive theaflavins, in mouse fecal samples using liquid chromatography/electrospray ionization tandem mass spectrometry by analyzing the MSn (n=1–3) spectra. Four metabolites theaflavin, theaflavin 3-gallate, theaflavin 3′-gallate, and gallic acid were identified as the major mouse fecal metabolites of TFDG. Glucuronidated and sulfated, instead of...
Preparation and evaluation of a porous monolithic capillary column for microextraction of estrogens from urine and milk samples online coupled to high-performance liquid chromatography
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Yuling, Hu , Yifeng, Fan , Gongke, Li
A porous monolithic capillary column based on poly (methacrylic acid-co-ethylene glycol dimethacrylate) (poly (MAA-co-EGDMA)) was prepared using methanol and polyethylene glycol 6000 as mixed porogens. The monolith has the characteristics of good permeability, high extraction efficiency and long lifetime. Improved permeability of the monolith could realize sample loading with high flow rate. A simple and convenient construction that employed valve-switch technique was designed for online coupling of the monolithic capillary column to high performance liquid chromatography. In order to obtain optimum extraction efficiency, the extraction conditions including sample pH, sample volume, extraction and desorption flow rate were investigated. Under the...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Yuling, Hu , Yifeng, Fan , Gongke, Li
A porous monolithic capillary column based on poly (methacrylic acid-co-ethylene glycol dimethacrylate) (poly (MAA-co-EGDMA)) was prepared using methanol and polyethylene glycol 6000 as mixed porogens. The monolith has the characteristics of good permeability, high extraction efficiency and long lifetime. Improved permeability of the monolith could realize sample loading with high flow rate. A simple and convenient construction that employed valve-switch technique was designed for online coupling of the monolithic capillary column to high performance liquid chromatography. In order to obtain optimum extraction efficiency, the extraction conditions including sample pH, sample volume, extraction and desorption flow rate were investigated. Under the...
Electromembrane Extraction of Stimulating Drugs from Undiluted Whole Blood
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
RagnhildElénGjulem, Jamt , Astrid, Gjelstad , Lars Erik Eng, Eibak , Elisabeth Leere, Øiestad , AsbjørgSolberg, Christophersen , ...
For the first time, electromembrane extraction (EME) of six basic drugs of abuse from undiluted whole blood and post mortem blood in a totally stagnant system is reported. Cathinone, methamphetamine, 3,4-methylenedioxy-amphetamine (MDA), 3,4-methylenedioxy-methamphet-amine (MDMA), ketamine and 2,5-dimethoxy-4-iodoamphetamine (DOI) were extracted from the whole blood sample, through a supported liquid membrane (SLM) consisting of 1-ethyl-2-nitrobenzene (ENB) immobilized in the pores of a hollow fiber, and into an aqueous acceptor solution inside the lumen of the hollow fiber. The SLM acts as a barrier with efficient exclusion of all macromolecules and acidic substances in the sample. Due to the application of the...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
RagnhildElénGjulem, Jamt , Astrid, Gjelstad , Lars Erik Eng, Eibak , Elisabeth Leere, Øiestad , AsbjørgSolberg, Christophersen , ...
For the first time, electromembrane extraction (EME) of six basic drugs of abuse from undiluted whole blood and post mortem blood in a totally stagnant system is reported. Cathinone, methamphetamine, 3,4-methylenedioxy-amphetamine (MDA), 3,4-methylenedioxy-methamphet-amine (MDMA), ketamine and 2,5-dimethoxy-4-iodoamphetamine (DOI) were extracted from the whole blood sample, through a supported liquid membrane (SLM) consisting of 1-ethyl-2-nitrobenzene (ENB) immobilized in the pores of a hollow fiber, and into an aqueous acceptor solution inside the lumen of the hollow fiber. The SLM acts as a barrier with efficient exclusion of all macromolecules and acidic substances in the sample. Due to the application of the...
Ultra high performance liquid chromatography-time of flight mass spectrometry for analysis of avocado fruit metabolites: method evaluation and applicability to the analysis of ripening degrees
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Elena, Hurtado-Fernández , Tiziana, Pacchiarotta , María, Gómez-Romero , Bart, Schoenmaker , Rico, Derks , ...
We have developed an analytical method using UHPLC-UV/ESI-TOF MS for the comprehensive profiling of the metabolites found in the methanolic extracts of 13 different varieties of avocado at two different ripening degrees. Both chromatographic and detection parameters were optimized in order to maximize the number of compounds detected and the sensitivity. After achieving the optimum conditions, we performed a complete analytical validation of the method with respect to its linearity, sensitivity, precision, accuracy and possible matrix effects. The LODs ranged from 1.64 to 730.54 ppb (in negative polarity) for benzoic acid and chrysin, respectively, whilst they were found within the...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
Elena, Hurtado-Fernández , Tiziana, Pacchiarotta , María, Gómez-Romero , Bart, Schoenmaker , Rico, Derks , ...
We have developed an analytical method using UHPLC-UV/ESI-TOF MS for the comprehensive profiling of the metabolites found in the methanolic extracts of 13 different varieties of avocado at two different ripening degrees. Both chromatographic and detection parameters were optimized in order to maximize the number of compounds detected and the sensitivity. After achieving the optimum conditions, we performed a complete analytical validation of the method with respect to its linearity, sensitivity, precision, accuracy and possible matrix effects. The LODs ranged from 1.64 to 730.54 ppb (in negative polarity) for benzoic acid and chrysin, respectively, whilst they were found within the...
Characteristic fragmentation patterns of trimethylsilyl and trimethylsilyl-oxime derivatives of plant disaccharides as obtained by gas chromatography coupled to ion-trap mass spectrometry
25 August 2011, 20:49:03
Publication year: 2011
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
I., Boldizsár , Zs., Füzfai , I., Molnár-Perl
The characteristic fragmentation pattern of six reducing and two non reducing type disaccharides - (neohesperidose, acuminose, sambubiose, rutinose, vicianose, primverose, and two arabinosyl-inositols) has been described. These saccharides have not been previously identified by on-line chromatographic techniques. Unambiguous specific characteristics of the TMS (oxime)’s such as mass distribution, syn/anti oximes ratios and elution order proved to be associated with their reducing or non reducing character, with their aldosyl property and with the position of their O-glycosidic linkages.The practical utility of the mass fragmentation study of these rare disaccharides was demonstrated, at the first time, by the simultaneous, on-line identification and...
Source: Journal of Chromatography A, In Press, Accepted Manuscript, Available online 25 August 2011
I., Boldizsár , Zs., Füzfai , I., Molnár-Perl
The characteristic fragmentation pattern of six reducing and two non reducing type disaccharides - (neohesperidose, acuminose, sambubiose, rutinose, vicianose, primverose, and two arabinosyl-inositols) has been described. These saccharides have not been previously identified by on-line chromatographic techniques. Unambiguous specific characteristics of the TMS (oxime)’s such as mass distribution, syn/anti oximes ratios and elution order proved to be associated with their reducing or non reducing character, with their aldosyl property and with the position of their O-glycosidic linkages.The practical utility of the mass fragmentation study of these rare disaccharides was demonstrated, at the first time, by the simultaneous, on-line identification and...
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