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Selected papers from the latest issue:Development Of a Technique Based On Multi-Spectral Imaging For Monitoring The Conservation Of Cultural Heritage Objects
08 September 2011, 20:28:21
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Emilio, Marengo , Marcello, Manfredi , Orfeo, Zerbinati , Elisa, Robotti , Eleonora, Mazzucco , ...
A new approach for monitoring the state of conservation of cultural heritage objects surfaces is being developed. The technique utilizes multi-spectral imaging, multivariate analysis and statistical process control theory for the automatic detection of a possible deterioration process, its localization and identification, and the wavelengths most sensitive to detecting this before the human eye can detect the damage or potential degradation changes occur. A series of virtual degradation analyses were performed on images of parchment in order to test the proposed algorithm in controlled conditions. The spectral image of a Dead Sea Scroll (DSS) parchment, IAA (Israel Antiquities Authority) inventory...Graphical abstract
Graphical abstract: Highlights: ► We develop a new approach for the monitoring of cultural heritage objects. ► The technique uses multispectral imaging coupled with multivariate analysis. ► We tested the technique on simulated data and on a sample of parchment artificially aged. ► The technique detected the degraded area and the interested wavelengths even for small degradations.
PREDICTION OF COMPOSITIONAL AND SENSORY CHARACTERISTICS USING RGB DIGITAL IMAGES AND MULTIVARIATE CALIBRATION TECHNIQUES
08 September 2011, 20:28:21Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
G., Foca , F., Masino , A., Antonelli , A., Ulrici
In the present paper, the possibility to use the information contained in RGB digital images to gain a fast and inexpensive quantification of colour-related properties of food is explored. To this aim, we present an approach which consists, as first step, in condensing the colour related information contained in RGB digital images of the analysed samples in one-dimensional signals, named colourgrams. These signals are then used as descriptor variables in multivariate calibration models. The feasibility of this approach has been tested using as a benchmark a series of samples of pesto sauce, whose RGB images have been used to predict...Graphical abstract
Graphical abstract: Highlights: ► Visual aspect of a sample can be quantified by RGB images ► Colour-related information is codified in the form of a signal named colourgram ► Colourgrams are used to build multivariate calibration/feature selection models ► Sensory and compositional characteristics of samples (e.g. food) can be predicted ► Reconstruction of selected features as images allow direct and easy interpretation
Aptamer Enzymatic Cleavage Protection Assay for the Gold Nanoparticle-Based Colorimetric Sensing of Small Molecules
08 September 2011, 20:28:21Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Valérie, Guieu , Corinne, Ravelet , Sandrine, Perrier , Zhenyu, Zhu , Simon, Cayez , ...
A label-free, homogeneous aptamer-based sensor strategy was designed for the facile colorimetric detection of small target molecules. The format relied on the target-induced protection of DNA aptamer from the enzymatic digestion and its transduction into a detectable signal through the length-dependent adsorption of single-stranded DNA onto unmodified gold nanoparticles (AuNPs). The proof-of-principle of the approach was established by employing the anti-tyrosinamide aptamer as a model functional nucleic acid. In the absence of target, the aptamer was cleaved by the phosphodiesterase I enzymatic probe, leading to the release of mononucleotides and short DNA fragments. These governed effective electrostatic stabilization of AuNPs...Graphical abstract
Graphical abstract: Highlights: ► Homogeneous aptasensor designed for colorimetric detection of small molecules. ► Format relied on target-induced protection of aptamer from enzymatic digestion. ► Transduction attained through adsorption of DNA onto gold nanoparticles. ► Sensing platform successfully applied to tyrosinamide and adenosine detection.
A strategy for constructing sensitive and renewable molecularly imprinted electrochemical sensors for melamine detection
08 September 2011, 20:28:21Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Jianping, Li , Zhiqiang, Chen , Yuping, Li
A novel strategy for preparing highly sensitive and easily renewable molecularly imprinted polymer (MIP) sensors was proposed. Using melamine (MA) as the template molecule, MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. MA was indirectly determined from the competition between the reactions of MA and horseradish peroxidase-labeled MA (MA-HRP) with the vacant cavities. The detection signals were amplified because of enzymatic reaction to the H2O2 catalytic oxidation. Sensitivity was markedly improved. Sensor renewal was achieved by a simple mechanical polishing of the sensitive film. The linear range for MA detection was...Graphical abstract
Graphical abstract: A strategy for preparing molecularly imprinted electrochemical sensors with a highly sensitive and easily renewable film is proposed. Film renewability was achieved by embedding MIP particles in a solid paraffin carbon paste, and the high sensitivity was detected using a highly sensitive enzyme amplifier. Highlights: ► A strategy for preparing molecularly imprinted electrochemical sensors with a highly sensitive and easily renewable film is proposed. ► MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. ► The detection signals were amplified because of enzymatic reaction to the H2O2 catalytic oxidation. ► Sensitive film renewability was achieved by embedding MIP particles in a solid paraffin carbon paste. ► Melamine was selected as the template molecule
Development Of a Technique Based On Multi-Spectral Imaging For Monitoring The Conservation Of Cultural Heritage Objects
08 September 2011, 20:28:21
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Emilio, Marengo , Marcello, Manfredi , Orfeo, Zerbinati , Elisa, Robotti , Eleonora, Mazzucco , ...
A new approach for monitoring the state of conservation of cultural heritage objects surfaces is being developed. The technique utilizes multi-spectral imaging, multivariate analysis and statistical process control theory for the automatic detection of a possible deterioration process, its localization and identification, and the wavelengths most sensitive to detecting this before the human eye can detect the damage or potential degradation changes occur. A series of virtual degradation analyses were performed on images of parchment in order to test the proposed algorithm in controlled conditions. The spectral image of a Dead Sea Scroll (DSS) parchment, IAA (Israel Antiquities Authority) inventory...
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Emilio, Marengo , Marcello, Manfredi , Orfeo, Zerbinati , Elisa, Robotti , Eleonora, Mazzucco , ...
A new approach for monitoring the state of conservation of cultural heritage objects surfaces is being developed. The technique utilizes multi-spectral imaging, multivariate analysis and statistical process control theory for the automatic detection of a possible deterioration process, its localization and identification, and the wavelengths most sensitive to detecting this before the human eye can detect the damage or potential degradation changes occur. A series of virtual degradation analyses were performed on images of parchment in order to test the proposed algorithm in controlled conditions. The spectral image of a Dead Sea Scroll (DSS) parchment, IAA (Israel Antiquities Authority) inventory...
Graphical abstract
Graphical abstract: Highlights: ► We develop a new approach for the monitoring of cultural heritage objects. ► The technique uses multispectral imaging coupled with multivariate analysis. ► We tested the technique on simulated data and on a sample of parchment artificially aged. ► The technique detected the degraded area and the interested wavelengths even for small degradations.PREDICTION OF COMPOSITIONAL AND SENSORY CHARACTERISTICS USING RGB DIGITAL IMAGES AND MULTIVARIATE CALIBRATION TECHNIQUES
08 September 2011, 20:28:21
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
G., Foca , F., Masino , A., Antonelli , A., Ulrici
In the present paper, the possibility to use the information contained in RGB digital images to gain a fast and inexpensive quantification of colour-related properties of food is explored. To this aim, we present an approach which consists, as first step, in condensing the colour related information contained in RGB digital images of the analysed samples in one-dimensional signals, named colourgrams. These signals are then used as descriptor variables in multivariate calibration models. The feasibility of this approach has been tested using as a benchmark a series of samples of pesto sauce, whose RGB images have been used to predict...
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
G., Foca , F., Masino , A., Antonelli , A., Ulrici
In the present paper, the possibility to use the information contained in RGB digital images to gain a fast and inexpensive quantification of colour-related properties of food is explored. To this aim, we present an approach which consists, as first step, in condensing the colour related information contained in RGB digital images of the analysed samples in one-dimensional signals, named colourgrams. These signals are then used as descriptor variables in multivariate calibration models. The feasibility of this approach has been tested using as a benchmark a series of samples of pesto sauce, whose RGB images have been used to predict...
Graphical abstract
Graphical abstract: Highlights: ► Visual aspect of a sample can be quantified by RGB images ► Colour-related information is codified in the form of a signal named colourgram ► Colourgrams are used to build multivariate calibration/feature selection models ► Sensory and compositional characteristics of samples (e.g. food) can be predicted ► Reconstruction of selected features as images allow direct and easy interpretationAptamer Enzymatic Cleavage Protection Assay for the Gold Nanoparticle-Based Colorimetric Sensing of Small Molecules
08 September 2011, 20:28:21
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Valérie, Guieu , Corinne, Ravelet , Sandrine, Perrier , Zhenyu, Zhu , Simon, Cayez , ...
A label-free, homogeneous aptamer-based sensor strategy was designed for the facile colorimetric detection of small target molecules. The format relied on the target-induced protection of DNA aptamer from the enzymatic digestion and its transduction into a detectable signal through the length-dependent adsorption of single-stranded DNA onto unmodified gold nanoparticles (AuNPs). The proof-of-principle of the approach was established by employing the anti-tyrosinamide aptamer as a model functional nucleic acid. In the absence of target, the aptamer was cleaved by the phosphodiesterase I enzymatic probe, leading to the release of mononucleotides and short DNA fragments. These governed effective electrostatic stabilization of AuNPs...
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Valérie, Guieu , Corinne, Ravelet , Sandrine, Perrier , Zhenyu, Zhu , Simon, Cayez , ...
A label-free, homogeneous aptamer-based sensor strategy was designed for the facile colorimetric detection of small target molecules. The format relied on the target-induced protection of DNA aptamer from the enzymatic digestion and its transduction into a detectable signal through the length-dependent adsorption of single-stranded DNA onto unmodified gold nanoparticles (AuNPs). The proof-of-principle of the approach was established by employing the anti-tyrosinamide aptamer as a model functional nucleic acid. In the absence of target, the aptamer was cleaved by the phosphodiesterase I enzymatic probe, leading to the release of mononucleotides and short DNA fragments. These governed effective electrostatic stabilization of AuNPs...
Graphical abstract
Graphical abstract: Highlights: ► Homogeneous aptasensor designed for colorimetric detection of small molecules. ► Format relied on target-induced protection of aptamer from enzymatic digestion. ► Transduction attained through adsorption of DNA onto gold nanoparticles. ► Sensing platform successfully applied to tyrosinamide and adenosine detection.A strategy for constructing sensitive and renewable molecularly imprinted electrochemical sensors for melamine detection
08 September 2011, 20:28:21
Publication year: 2011
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Jianping, Li , Zhiqiang, Chen , Yuping, Li
A novel strategy for preparing highly sensitive and easily renewable molecularly imprinted polymer (MIP) sensors was proposed. Using melamine (MA) as the template molecule, MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. MA was indirectly determined from the competition between the reactions of MA and horseradish peroxidase-labeled MA (MA-HRP) with the vacant cavities. The detection signals were amplified because of enzymatic reaction to the H2O2 catalytic oxidation. Sensitivity was markedly improved. Sensor renewal was achieved by a simple mechanical polishing of the sensitive film. The linear range for MA detection was...
Source: Analytica Chimica Acta, In Press, Accepted Manuscript, Available online 7 September 2011
Jianping, Li , Zhiqiang, Chen , Yuping, Li
A novel strategy for preparing highly sensitive and easily renewable molecularly imprinted polymer (MIP) sensors was proposed. Using melamine (MA) as the template molecule, MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. MA was indirectly determined from the competition between the reactions of MA and horseradish peroxidase-labeled MA (MA-HRP) with the vacant cavities. The detection signals were amplified because of enzymatic reaction to the H2O2 catalytic oxidation. Sensitivity was markedly improved. Sensor renewal was achieved by a simple mechanical polishing of the sensitive film. The linear range for MA detection was...
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