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Selected papers from the latest issue:MCX based solid phase extraction combined with liquid chromatography tandem mass spectrometry for the simultaneous determination of 31 endocrine-disrupting compounds in surface water of Shanghai
04 September 2011, 20:14:09
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Hong-Chang, Zhang , Xue-jun, Yu , Wen-chao, Yang , Jin-feng, Peng , Ting, Xu , ...
A novel analytical method employing MCX (mixed-mode cationic exchange) based solid phase extraction (SPE) coupled with liquid chromatography tandom mass spectrometry (LC-MS/MS) was developed to detect 31 endocrine-disrupting compounds (EDCs) in surface water samples simultaneously. The target EDCs belong to five classes, including seven estrogens, eight androgens, six progesterones, five adrenocortical hormones and five industrial compounds. In order to simultaneously concentrate the target EDCs and eliminate matrix interferences in the water samples, MCX SPE cartridges were employed for SPE, and then followed by a simple and highly efficient three-step sequential elution procedure. Two electrospray ionization (ESI) detection modes, positive (ESI+)...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Hong-Chang, Zhang , Xue-jun, Yu , Wen-chao, Yang , Jin-feng, Peng , Ting, Xu , ...
A novel analytical method employing MCX (mixed-mode cationic exchange) based solid phase extraction (SPE) coupled with liquid chromatography tandom mass spectrometry (LC-MS/MS) was developed to detect 31 endocrine-disrupting compounds (EDCs) in surface water samples simultaneously. The target EDCs belong to five classes, including seven estrogens, eight androgens, six progesterones, five adrenocortical hormones and five industrial compounds. In order to simultaneously concentrate the target EDCs and eliminate matrix interferences in the water samples, MCX SPE cartridges were employed for SPE, and then followed by a simple and highly efficient three-step sequential elution procedure. Two electrospray ionization (ESI) detection modes, positive (ESI+)...
A New Strategy for the On-column Exopeptidase Cleavage of Poly-Histidine Tagged Proteins
04 September 2011, 20:14:09
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Wen-Hui K., Kuo , Howard A., Chase
This paper describes a new strategy, which aims to make on-column poly-histidine tag removal more useful in the production of recombinant proteins by improving the yield and efficiency of on-column exopeptidase cleavage. This involves improvement of the on-column cleavage condition by using imidazole concentrations in the range of 100mM to 500mM in the cleavage buffer. At 300mM imidazole, maximum on-column cleavage yield (in excess of 99%) was achieved in 3hours of incubation. However, as a result of the increased imidazole concentration, this new strategy of on-column cleavage results in some residual uncleaved poly-histidine tagged proteins (∼0.1%) and the production of...
Highlights: ► A new strategy to improve the yield and efficiency of on-column exopeptidase cleavage is described. ► Maximum on-column cleavage yield in excess of 99% was achieved in 3hours of incubation at 300mM imidazole. ► The effect of protease concentration and incubation time on the new strategy is significant. ► Comparisons are made for different tag removal strategies.
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Wen-Hui K., Kuo , Howard A., Chase
This paper describes a new strategy, which aims to make on-column poly-histidine tag removal more useful in the production of recombinant proteins by improving the yield and efficiency of on-column exopeptidase cleavage. This involves improvement of the on-column cleavage condition by using imidazole concentrations in the range of 100mM to 500mM in the cleavage buffer. At 300mM imidazole, maximum on-column cleavage yield (in excess of 99%) was achieved in 3hours of incubation. However, as a result of the increased imidazole concentration, this new strategy of on-column cleavage results in some residual uncleaved poly-histidine tagged proteins (∼0.1%) and the production of...
Highlights: ► A new strategy to improve the yield and efficiency of on-column exopeptidase cleavage is described. ► Maximum on-column cleavage yield in excess of 99% was achieved in 3hours of incubation at 300mM imidazole. ► The effect of protease concentration and incubation time on the new strategy is significant. ► Comparisons are made for different tag removal strategies.
Fabrication of a novel nanocomposite based on sol–gel process for hollow fiber–solid phase microextraction of aflatoxins;B1 and B2, in cereals combined with high performane liquid chromatography- diode array detection
04 September 2011, 20:14:09
Publication year: 2011
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Zarrin, Es’haghi , Hoda, Sorayaei , Fateme, Samadi , Mahboubeh, Masrournia , Zohreh, Bakherad
The new pre-concentration technique, hollow fiber-solid phase microextraction based on carbon nanotube reinforced sol-gel and liquid chromatography-photodiode array detection was applied to determination of aflatoxins B1,B2 (AFB1, AFB2) in rice, peanut and wheat samples. This research provides an overview of trends related to synthesis of solid phase microextraction (SPME) sorbnents that improves the assay of aflatoxins as the semi-polar compounds in several real samples. It mainly includes summary and a list of the results for a simple carbon nanotube reinforced sol-gel in-fiber device. This device was used for extraction, pre-concentration and determination of aflatoxins B1,B2 in real samples. In this...
Source: Journal of Chromatography B, In Press, Accepted Manuscript, Available online 3 September 2011
Zarrin, Es’haghi , Hoda, Sorayaei , Fateme, Samadi , Mahboubeh, Masrournia , Zohreh, Bakherad
The new pre-concentration technique, hollow fiber-solid phase microextraction based on carbon nanotube reinforced sol-gel and liquid chromatography-photodiode array detection was applied to determination of aflatoxins B1,B2 (AFB1, AFB2) in rice, peanut and wheat samples. This research provides an overview of trends related to synthesis of solid phase microextraction (SPME) sorbnents that improves the assay of aflatoxins as the semi-polar compounds in several real samples. It mainly includes summary and a list of the results for a simple carbon nanotube reinforced sol-gel in-fiber device. This device was used for extraction, pre-concentration and determination of aflatoxins B1,B2 in real samples. In this...
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