Invited Review, submitted for consideration to The Journal of Chromatography A Technology Trends in Antibody Purification
21 October 2011, 03:11:25
Publication year: 2011
Source: Journal of Chromatography A, Available online 20 October 2011
Pete Gagnon
This article reviews technology trends in antibody purification. The first section discusses non-chromatography methods, including precipitation, liquid-liquid extraction, and high performance tangential flow filtration. The second addresses chromatography methods. It begins with discussion of fluidized and fixed bed formats. It continues with stationary phase architecture: diffusive particles, perfusive particles, membranes and monoliths. The remainder of the section reviews recent innovations in size exclusion, anion exchange, cation exchange, hydrophobic interaction, immobilized metal affinity, mixed-mode, and bioaffinity chromatography. The third section addresses an emerging trend of formulating process buffers to prevent or correct anomalies in the antibodies being purified. Methods are discussed for preventing aggregate formation, dissociating antibody-contaminant complexes, restoring native antibody from aggregates, and conserving or restoring native disulfide pairing.
Source: Journal of Chromatography A, Available online 20 October 2011
Pete Gagnon
This article reviews technology trends in antibody purification. The first section discusses non-chromatography methods, including precipitation, liquid-liquid extraction, and high performance tangential flow filtration. The second addresses chromatography methods. It begins with discussion of fluidized and fixed bed formats. It continues with stationary phase architecture: diffusive particles, perfusive particles, membranes and monoliths. The remainder of the section reviews recent innovations in size exclusion, anion exchange, cation exchange, hydrophobic interaction, immobilized metal affinity, mixed-mode, and bioaffinity chromatography. The third section addresses an emerging trend of formulating process buffers to prevent or correct anomalies in the antibodies being purified. Methods are discussed for preventing aggregate formation, dissociating antibody-contaminant complexes, restoring native antibody from aggregates, and conserving or restoring native disulfide pairing.
Multidimensional Enantio Gas Chromtography/Mass Spectrometry and Gas Chromatography-Combustion-Isotopic Ratio Mass Spectrometry for the Authenticity Assessment of Lime Essential Oils (C. aurantifoliaSwingle andC. latifoliaTanaka)
21 October 2011, 03:11:25
Publication year: 2011
Source: Journal of Chromatography A, Available online 20 October 2011
Ivana Bonaccorsi, Danilo Sciarrone, Luisa Schipilliti, Paola Dugo, Luigi Mondello, ...
This article focuses on the genuineness assessment of Lime oils (C. aurantifoliaSwingle andC. latifoliaTanaka), by Multi Dimensional Gas Chromatography (MDGC) to determine the enantiomeric distribution of α-thujene, camphene, β-pinene, sabinene, α-phellandrene, β-phellandrene, limonene, linalool, terpinen-4-ol, α-terpineol and by Gas Chromatography Combustion Isotope Ratio Mass Spectrometry (GC-C-IRMS) to determine the isotopic ratios of α-pinene, β-pinene, limonene, α-terpineol, neral, geranial,β-caryophyllene, trans-α-bergamotene, germacrene B. To the author's knowledge this is the first attempt to assess the authenticity and differentiate Persian Lime from Key lime oils by GC-C-IRMS. The results of the two analytical approaches were compared. The simultaneous use of the two techniques provides more reliable capability to detect adulteration inCitrusessential oils. In fact, in some circumstance only one of the two techniques allows to discriminate adulterated or contaminated oils. In cases where only small anomalies are detected by the two techniques due to subtle adulterations, their synergic use allows to express judgments. The advantage of both techniques is the low number of components the analyst must evaluate, reducing the complexity of the data necessary to deal with. Moreover, the conventional analytical approach based on the evaluation of the whole volatile fraction can fail to reveal the quality of the oils, if the adulteration is extremely subtle.
Source: Journal of Chromatography A, Available online 20 October 2011
Ivana Bonaccorsi, Danilo Sciarrone, Luisa Schipilliti, Paola Dugo, Luigi Mondello, ...
This article focuses on the genuineness assessment of Lime oils (C. aurantifoliaSwingle andC. latifoliaTanaka), by Multi Dimensional Gas Chromatography (MDGC) to determine the enantiomeric distribution of α-thujene, camphene, β-pinene, sabinene, α-phellandrene, β-phellandrene, limonene, linalool, terpinen-4-ol, α-terpineol and by Gas Chromatography Combustion Isotope Ratio Mass Spectrometry (GC-C-IRMS) to determine the isotopic ratios of α-pinene, β-pinene, limonene, α-terpineol, neral, geranial,β-caryophyllene, trans-α-bergamotene, germacrene B. To the author's knowledge this is the first attempt to assess the authenticity and differentiate Persian Lime from Key lime oils by GC-C-IRMS. The results of the two analytical approaches were compared. The simultaneous use of the two techniques provides more reliable capability to detect adulteration inCitrusessential oils. In fact, in some circumstance only one of the two techniques allows to discriminate adulterated or contaminated oils. In cases where only small anomalies are detected by the two techniques due to subtle adulterations, their synergic use allows to express judgments. The advantage of both techniques is the low number of components the analyst must evaluate, reducing the complexity of the data necessary to deal with. Moreover, the conventional analytical approach based on the evaluation of the whole volatile fraction can fail to reveal the quality of the oils, if the adulteration is extremely subtle.
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