Analytical Chemist

Review

Electrophoresis and Electrochromatography

2.

Capillary electrophoresis methods for the analysis of antimalarials. Part II. Achiral separative methods   Review Article Pages 1-11 N’Cho Christophe Amin, Marie-Dominique Blanchin, Michèle Aké, Huguette Fabre Highlights ► Exhaustive review of applications of achiral CE methods for antimalarials. ► Analysis in pharmaceutical formulations, biological fluids and food. ► Determination of physico-chemical properties.

Regular Papers

Sample Preparation

3.

Automated determination of phenolic compounds in wine, berry, and grape samples using 96-blade solid phase microextraction system coupled with liquid chromatography–tandem mass spectrometry   Original Research Article Pages 12-19 Fatemeh S. Mirnaghi, Fatemeh Mousavi, Sílvia M. Rocha, Janusz Pawliszyn Highlights ► An automated SPME system based on thin film geometry is optimized for food analysis. ► High throughput analysis of phenolic compounds in 96-well format is achieved. ► The thin-film SPME system provided high sensitivity and low limits of detection. ► The method was applied for analysis of phenolics in wine, berry, and grape samples.

Column Liquid Chromatography

4.

Determination of ethylenediaminetetraacetic acid in nuclear waste by high-performance liquid chromatography coupled with electrospray mass spectrometry   Original Research Article Pages 20-25 Laurence du Bois de Maquillé, Laetitia Renaudin, Florence Goutelard, Alain Jardy, Jérôme Vial, Didier Thiébaut Highlights ► We make speciation studies to determine the behavior of EDTA in complex matrix. ► Fe precipitates at pH lower than 2 in nuclear waste. ► Ni is the cation for EDTA quantification by HPLC–ESI-MS. ► The method proved to be accurate and precise for the analysis in nuclear waste.

5.

Simple automated liquid chromatographic system for splitless nano column gradient separations   Original Research Article Pages 26-32 Jozef Šesták, Filip Duša, Dana Moravcová, Vladislav Kahle Highlights ► We assembled a simple splitless gradient nano liquid chromatographic system. ► Capability of automated system to create various gradient profiles was verified. ► System was tested by mixture of alkylphenones and by BSA tryptic digest. ► A high repeatability of retention times (RSD ∼ 0.3%) was obtained. ► A sample volumes from 0.1 to 3μl could be injected with RSD<5%.

6.

Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography   Original Research Article Pages 33-46 Abhinav Kumar, James C. Heaton, David V. McCalley Highlights ► We compare the retention characteristics of 6 different HILIC columns. ► We measure the effects of experimental variables on selectivity. ► The stationary phase has the greatest effect on selectivity. ► Buffer pH also has considerable influence for ionised solutes. ► Solute logDvalues are only a partial predictor of retention.

7.

Qualitative validation of a liquid chromatography–quadrupole-time of flight mass spectrometry screening method for organic pollutants in waters   Original Research Article Pages 47-57 R. Diaz, M. Ibáñez, J.V. Sancho, F. Hernández Highlights ► Qualitative validation of a UHPLC–(Q)TOF MS wide-scope screening method performed. ► Contaminant identification criteria have been discussed for HRMS methodologies. ► SDL and LOI have been established for around 150 contaminants from different families. ► The methodology have been applied to real waters samples.

8.

Single step plasmid DNA purification using methacrylate monolith bearing combination of ion-exchange and hydrophobic groups   Original Research Article Pages 58-64 Vida Smrekar, Franc Smrekar, Aleš Štrancar, Aleš Podgornik Highlights ► Single step purification of pDNA on miligram scale. ► Characterization of grafted HIC groups. ► Multidimensional purification of pDNA. ► Very fast separation of pDNA using monoliths.

9.

Quantitative structure–retention relationships applied to liquid chromatography gradient elution method for the determination of carbonyl-2,4-dinitrophenylhydrazone compounds   Original Research Article Pages 65-77 Elisenda Cirera-Domènech, Roger Estrada-Tejedor, Francesc Broto-Puig, Jordi Teixidó, Miquel Gassiot-Matas, Lluís Comellas, Josep Lluís Lliberia, Alberto Méndez, Susanna Paz-Estivill, Maria Rosa Delgado-Ortiz Highlights ► Good QSRR correlation models were found for gradient and isocratic HPLC-UV methods. ► QSRR models were used to identify unknown compounds in experimental samples. ► A tool to classify carbonyl-DNPH compounds in different families was proposed.

10.

Liquid chromatography coupled to tandem mass spectrometry and high resolution mass spectrometry as analytical tools to characterize multi-class cytostatic compounds   Original Research Article Pages 78-94 Cristian Gómez-Canela, Nuria Cortés-Francisco, Francesc Ventura, Josep Caixach, Silvia Lacorte Highlights ► Cytostatic compounds were fully characterized by LC–MS/MS and LC-Orbitrap-MS. ► Good chromatographic resolution was obtained atRs=50,000 FWHM. ► Limits of detection ranged from 0.005 to 0.15ng, being Orbitrap more sensitive. ► The multiresidual analytical method can be applied in monitoring studies.

11.

Effects of extraction and high-performance liquid chromatographic conditions on the determination of lutein in spinach   Original Research Article Pages 95-101 Breda Simonovska, Irena Vovk, Vesna Glavnik, Katarina Černelič Highlights ► Triethylammonium acetate buffer pH 7 in the aqueous acetone for HPLC of carotenoids. ► The same mixture improved extraction of lutein from spinach compared to pure acetone. ► Separation of all spinach pigments in less than 15min on core–shell C18 column.

12.

Chelation ion chromatography of alkaline earth and transition metals a using monolithic silica column with bonded N-hydroxyethyliminodiacetic acid functional groups   Original Research Article Pages 102-111 Nicola McGillicuddy, Ekaterina P. Nesterenko, Pavel N. Nesterenko, Phil Jones, Brett Paull Highlights ► Reports improved procedure for production of HEIDA monolithic silica columns. ► New column exhibits improved capacity and efficiency for metal ions. ► Column offers unique selectivity for alkaline earth and transition metal cations. ► Results demonstrate multiple ways to manipulate separation selectivity. ► Speciation calculations explain observed retention trends with complexing eluents.

Gas Chromatography

13.

Separation performance of guanidinium-based ionic liquids as stationary phases for gas chromatography   Original Research Article Pages 112-119 Lizhen Qiao, Kai Lu, Meiling Qi, Ruonong Fu Highlights ► Guanidinium-based ionic liquids (GBILs) are presented as new GC stationary phases. ► GBILs show excellent separation performance and thermal stability. ► GBILs have different retention behaviors from the widely used stationary phases. ► GBILs can be used for separation of analytes of various nature.

Electrophoresis and Electrochromatography

14.

Uranyl complexation with selenate at variable temperatures studied by affinity capillary electrophoresis   Original Research Article Pages 120-125 Vladimir Sladkov Highlights ► Uranyl complexation with selenate studied at variable temperatures. ► Complex species become stronger as the temperature is increased. ► Thermodynamic parameters are reported for uranyl–selenate system for the first time. ► The complexation is endothermic and entropy-driven.