World Congress on Biosensors 2014

World Congress on Biosensors 2014
Biosensors 2014

Friday, 8 February 2013


  Electrophoresis and Electrochromatography

Capillary electrophoresis methods for the analysis of antimalarials. Part II. Achiral separative methods   Review Article

Pages 1-11
N’Cho Christophe Amin, Marie-Dominique Blanchin, Michèle Aké, Huguette Fabre


► Exhaustive review of applications of achiral CE methods for antimalarials. ► Analysis in pharmaceutical formulations, biological fluids and food. ► Determination of physico-chemical properties.

  Regular Papers

  Sample Preparation



► An automated SPME system based on thin film geometry is optimized for food analysis. ► High throughput analysis of phenolic compounds in 96-well format is achieved. ► The thin-film SPME system provided high sensitivity and low limits of detection. ► The method was applied for analysis of phenolics in wine, berry, and grape samples.

  Column Liquid Chromatography

Determination of ethylenediaminetetraacetic acid in nuclear waste by high-performance liquid chromatography coupled with electrospray mass spectrometry   Original Research Article

Pages 20-25
Laurence du Bois de Maquillé, Laetitia Renaudin, Florence Goutelard, Alain Jardy, Jérôme Vial, Didier Thiébaut


► We make speciation studies to determine the behavior of EDTA in complex matrix. ► Fe precipitates at pH lower than 2 in nuclear waste. ► Ni is the cation for EDTA quantification by HPLC–ESI-MS. ► The method proved to be accurate and precise for the analysis in nuclear waste.

Simple automated liquid chromatographic system for splitless nano column gradient separations   Original Research Article

Pages 26-32
Jozef Šesták, Filip Duša, Dana Moravcová, Vladislav Kahle


► We assembled a simple splitless gradient nano liquid chromatographic system. ► Capability of automated system to create various gradient profiles was verified. ► System was tested by mixture of alkylphenones and by BSA tryptic digest. ► A high repeatability of retention times (RSD 0.3%) was obtained. ► A sample volumes from 0.1 to 3μl could be injected with RSD<5%.

Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography   Original Research Article

Pages 33-46
Abhinav Kumar, James C. Heaton, David V. McCalley


► We compare the retention characteristics of 6 different HILIC columns. ► We measure the effects of experimental variables on selectivity. ► The stationary phase has the greatest effect on selectivity. ► Buffer pH also has considerable influence for ionised solutes. ► Solute logDvalues are only a partial predictor of retention.



► Qualitative validation of a UHPLC–(Q)TOF MS wide-scope screening method performed. ► Contaminant identification criteria have been discussed for HRMS methodologies. ► SDL and LOI have been established for around 150 contaminants from different families. ► The methodology have been applied to real waters samples.

Single step plasmid DNA purification using methacrylate monolith bearing combination of ion-exchange and hydrophobic groups   Original Research Article

Pages 58-64
Vida Smrekar, Franc Smrekar, Aleš Štrancar, Aleš Podgornik


► Single step purification of pDNA on miligram scale. ► Characterization of grafted HIC groups. ► Multidimensional purification of pDNA. ► Very fast separation of pDNA using monoliths.

Quantitative structure–retention relationships applied to liquid chromatography gradient elution method for the determination of carbonyl-2,4-dinitrophenylhydrazone compounds   Original Research Article

Pages 65-77
Elisenda Cirera-Domènech, Roger Estrada-Tejedor, Francesc Broto-Puig, Jordi Teixidó, Miquel Gassiot-Matas, Lluís Comellas, Josep Lluís Lliberia, Alberto Méndez, Susanna Paz-Estivill, Maria Rosa Delgado-Ortiz


► Good QSRR correlation models were found for gradient and isocratic HPLC-UV methods. ► QSRR models were used to identify unknown compounds in experimental samples. ► A tool to classify carbonyl-DNPH compounds in different families was proposed.

Liquid chromatography coupled to tandem mass spectrometry and high resolution mass spectrometry as analytical tools to characterize multi-class cytostatic compounds   Original Research Article

Pages 78-94
Cristian Gómez-Canela, Nuria Cortés-Francisco, Francesc Ventura, Josep Caixach, Silvia Lacorte


► Cytostatic compounds were fully characterized by LC–MS/MS and LC-Orbitrap-MS. ► Good chromatographic resolution was obtained atRs=50,000 FWHM. ► Limits of detection ranged from 0.005 to 0.15ng, being Orbitrap more sensitive. ► The multiresidual analytical method can be applied in monitoring studies.

Effects of extraction and high-performance liquid chromatographic conditions on the determination of lutein in spinach   Original Research Article

Pages 95-101
Breda Simonovska, Irena Vovk, Vesna Glavnik, Katarina Černelič


► Triethylammonium acetate buffer pH 7 in the aqueous acetone for HPLC of carotenoids. ► The same mixture improved extraction of lutein from spinach compared to pure acetone. ► Separation of all spinach pigments in less than 15min on core–shell C18 column.

Chelation ion chromatography of alkaline earth and transition metals a using monolithic silica column with bonded N-hydroxyethyliminodiacetic acid functional groups   Original Research Article

Pages 102-111
Nicola McGillicuddy, Ekaterina P. Nesterenko, Pavel N. Nesterenko, Phil Jones, Brett Paull


► Reports improved procedure for production of HEIDA monolithic silica columns. ► New column exhibits improved capacity and efficiency for metal ions. ► Column offers unique selectivity for alkaline earth and transition metal cations. ► Results demonstrate multiple ways to manipulate separation selectivity. ► Speciation calculations explain observed retention trends with complexing eluents.

  Gas Chromatography

Separation performance of guanidinium-based ionic liquids as stationary phases for gas chromatography   Original Research Article

Pages 112-119
Lizhen Qiao, Kai Lu, Meiling Qi, Ruonong Fu


► Guanidinium-based ionic liquids (GBILs) are presented as new GC stationary phases. ► GBILs show excellent separation performance and thermal stability. ► GBILs have different retention behaviors from the widely used stationary phases. ► GBILs can be used for separation of analytes of various nature.

  Electrophoresis and Electrochromatography



► Uranyl complexation with selenate studied at variable temperatures. ► Complex species become stronger as the temperature is increased. ► Thermodynamic parameters are reported for uranyl–selenate system for the first time. ► The complexation is endothermic and entropy-driven.

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