Just Published: Biosensors & Bioelectronics

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here: Biosensors & Bioelectronics http://rss.sciencedirect.com/publication/science/5223 Selected papers from the latest issue:   Review Paper 2. Recent advances in olfactory receptor-basedbiosensors   Review Article Pages 570-580 Liping Du, Chunsheng Wu, Qingjun Liu, Liquan Huang, Ping Wang Highlights ► This review paper introduces the history of olfactory receptor-based biosensors comprehensively. ► Two hot issues in the development of olfactory receptor-based biosensors are discussed in detail. ► The latest developments of various olfactory receptor-based biosensors are presented. ► The future research trends are pointedout.   Full Papers 3. Fabrication of nanoporous thin-film working electrodes and their biosensingapplications   Original Research Article Pages 5-11 Tingjie Li, Falong Jia, Yaxi Fan, Zhifeng Ding, Jun Yang Highlights ► Nanoporous thin-film Au electrodes are fabricated by an alloying/dealloying process. ► Such nanoporous electrodes act as ideal platforms for immobilization of catalysts and enzymes. ► Electrochemical biosensors with such nanoporous electrodes have showed excellent performance. 4. Sensitive electrochemical thrombin aptasensor based on gold disk microelectrodearrays   Original Research Article Pages 17-22 Huei-Yu Bai, F. Javier Del Campo, Yu-Chen Tsai Highlights ► Gold disk microelectrode arrays for aptasensing improve both the detection limit and the sensor dynamic range. ► This method is able to selectively detect thrombin. ► Acceptable results are obtained for the proposed aptasensor to determine thrombin in synthetic blood serum. ► This label-free electrochemical aptasensor is potential for detections of other proteins and small molecules. 5. Microfluidic beads-based immunosensor for sensitive detection of cancer biomarker proteins using multienzyme-nanoparticle amplification and quantum dotslabels   Original Research Article Pages 23-30 He Zhang, Lian Liu, Xin Fu, Zhenjun Zhu Highlights ► Combination of microfluidic bead-based platform and enzyme–antibody-AuNPs is proposed. ► The developed immunosensor could respond to 0.2pgmL−1of AFP antigen. ► Microfluidic platform and multienzyme-labeled AuNPs greatly enhanced sensitivity. ► The accuracy of proposed immunosensor was evaluated by comparing with ELISA. ► We report an ultrasensitive immunosensor for detection of cancer biomarkers. 6. Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label free nuclease enzymedetection   Original Research Article Pages 31-35 Rong Hu, Ya-Ru Liu, Rong-Mei Kong, Michael J. Donovan, Xiao-Bing Zhang, Weihong Tan, Guo-Li Shen, Ru-Qin Yu Highlights ► A fluorescent probe based on double-strand DNA-templated formation of copper nanoparticles was developed for label-free nuclease enzyme detection. ► The probe shows a high sensitivity to S1 nuclease activity with a low detection limit of 0.3UmL−1observed. ► It also exhibits high selectivity to S1 nuclease over other nucleases. 7. A dual fluorometric and colorimetric sensor for dopamine based on BSA-stabilized Aunanoclusters   Original Research Article Pages 41-46 Yu Tao, Youhui Lin, Jinsong Ren, Xiaogang Qu Highlights ► A fluorescence assay for dopamine is developed based on the fluorescence quenching of BSA-AuNCs. ► A colorimetric approach for dopamine detection can also be realized. ► The colorimetric assay is based on the inhibition of the peroxidase-like activity of AuNCs. ► This method can be successfully used for the determination of dopamine in real samples. 8. Ultrasensitive detection of melamine based on a DNA-labeledimmunosensor   Original Research Article Pages 51-55 Honghong Yin, Yinyue Zhu, Liguang Xu, Hua Kuang, Libing Wang, Chuanlai Xu Highlights ► A DNA-labeled immunosensor for detection of melamine based on PCR amplification was developed. ► The sensor could achieve a low limit of detection of 0.3fgg−1in the range of 0.001–10pgg−1. ► The results showed the simple operations and high-throughput detections. 9. Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signalamplification   Original Research Article Pages 56-61 Yongxi Zhao, Feng Chen, Yayan Wu, Yanhua Dong, Chunhai Fan Highlights ► A fluorescence method was developed for screening Dam MTase activity and inhibition. ► It was based on methylation-sensitive cleavage coupled with nickase-assisted amplification. ► This strategy exhibited high sensitivity and excellent selectivity toward Dam MTase. ► It was also successfully employed to determine target in biological samples. ► The sensor can be extended as a universal platform for the assay of other DNA MTases. 10. Development of an electrochemical insulin sensor based on the insulin-linked polymorphicregion   Original Research Article Pages 62-68 Jennifer Y. Gerasimov, Cody S. Schaefer, Weiwei Yang, Rebecca L. Grout, Rebecca Y. Lai Highlights ► Design and fabrication of an electrochemical insulin sensor using a naturally occurring aptamer. ► Use of circular dichroism spectroscopy to determine structural changes upon insulin binding. ► The sensor binds insulin in a concentration-dependent manner. ► Insulin binding induces minor structural changes in the ILPR aptamer. ► Systematic approach to sensor characterization can be used to study electrochemical aptasensors. 11. A point-of-care PCR test for HIV-1 detection in resource-limitedsettings   Original Research Article Pages 69-75 Sujit R. Jangam, Abhishek K. Agarwal, Kunal Sur, David M. Kelso Highlights ► A point-of-care platform for PCR based HIV-1 testing is presented. ► Platform integrates DNA extraction, amplification and detection. ► A microfluidic assay card is used to assemble the reaction, thermocycle and detect fluorescence. ► A battery operated portable analyzer enables PCR based HIV-1 testing to be deployed in rural clinics. ► Point-of-care testing has the potential to reduce infant mortality by providing results to the caregivers in the samevisit. 12. Construction of a novel macroporous imprinted biosensor based on quartz crystal microbalance for ribonuclease Adetection   Original Research Article Pages 80-86 Shuai Liu, Dezhong Zhou, Tianying Guo Graphical abstract Highlights ► We develop a QCM biosensor based on porous MIP film for ribonuclease A determination. ► We use fluoromonomer as the matrix, N-methacryloyl-histidine as functional monomer. ► The selectivity factor and response feature was improved obviously for the new system. ► The rigid skeleton structure made the films durable in the recycledtests. 13. An enzyme substrate binding aptamer complex based time-resolved fluorescence sensor for the adenosine deaminasedetection   Original Research Article Pages 87-92 Kai Zhang, Qianlu Yang, Jue Zhang, Lixin Fu, Yan Zhou, Bing Wu, Minhao Xie, Biao Huang Highlights ► A strategy for the fabrication of a time-resolved fluorescent aptasensor is proposed. ► The label (Eu3+ions) has a longer lifetime on the order of milliseconds. ► It is a signal-on sensor with 0.5UL−1sensitivity for the detection of adenosine deaminase. 14. Enzyme-amplified electronic logic gates based on split/intactaptamers   Original Research Article Pages 93-99 Junhua Chen, Lingwen Zeng Highlights ► A series of enzyme-amplified electronic logic gates (OR, AND, NOR, and NAND) has been constructed at the molecular level. ► The two inputs are ATP and thrombin, the output is the amplified electronic signal. ► The electronic logic system is robust and can be applied to human serum samples with excellent selectivity. ► The reversibility of the logic gates makes the electronic system feasible to perform the set–reset function. 15. Zwitterionic polymer-modified silicon microring resonators for label-free biosensing in undiluted humanplasma   Original Research Article Pages 100-105 James T. Kirk, Norman D. Brault, Tom Baehr-Jones, Michael Hochberg, Shaoyi Jiang, Daniel M. Ratner Highlights ► Microring resonator biosensors were coated with zwitterionic polymer coatings. ► A “graft-to-surface” modification strategy was implemented to coat sensors. ► Coatings were demonstrated to be ultralow fouling in undiluted human plasma. ► Zwitterionic polymer coatings were functionalized with molecular capture elements. ► Clinically-relevant levels of protein were   detected in buffer and 100% human plasma. 16. Simultaneous detection of biomolecular interactions and surface topography using photonic forcemicroscopy   Original Research Article Pages 106-111 Seungjin Heo, Kipom Kim, Rodriguez Christophe, Tae-Young Yoon, Yong-Hoon Cho Highlights ► We developed a photonic force microscope that can map multiple parameters simultaneously. ► We demonstrated simultaneous detection of the surface topography and biomolecular interactions. ► We expect that this technique can be applied to detect and map multiple parameters of soft matter and biomaterials. 17. Graphene oxide/poly-l-lysine assembled layer for adhesion and electrochemical impedance detection of leukemia K562 cancercells   Original Research Article Pages 112-118 Dongdong Zhang, Yanmin Zhang, Lei Zheng, Yingzhuan Zhan, Langchong He Highlights ► A biocompatible interface of PLL/GO was developed for adhesion and detection of K562 cancer cells. ► The PLL/GO interface showed improved immobilization capacity and biocompatibility for cells. ► The obtained Nilotinib cytotoxicity results on K562 cells by proposed EIS method are satisfied. ► The proposed EIS method is free dyeing process, non-invasive, low-cost, labor- and time-saving. 18. Acetylcholinesterase biosensor based on chitosan/prussian blue/multiwall carbon nanotubes/hollow gold nanospheres nanocomposite film by one-stepelectrodeposition   Original Research Article Pages 124-130 Chen Zhai, Xia Sun, Wenping Zhao, Zhili Gong, Xiangyou Wang Highlights ► The Chit–PB–MWNTs–HGNs film was fabricated by one-step electrodeposition method. ► AChE and Nafion were modified onto the composite film to fabricate AChE biosensor. ► Application on four vegetables samples, the biosensor exhibited a good performance. 19. Highly sensitive detection of microRNAs based on isothermal exponential amplification-assisted generation of catalytic G-quadruplexDNAzyme   Original Research Article Pages 131-135 Xin-Ping Wang, Bin-Cheng Yin, Ping Wang, Bang-Ce Ye Highlights ► This paper presents a signal amplification method for nucleic acid detection. ► The amplification method is based on isothermal exponential amplification-assisted generation of catalytic G-quadruplex DNAzyme.► This paper demonstrates its practical application for microRNAs determination with detect limit of 1.0fM.