World Congress on Biosensors 2014

World Congress on Biosensors 2014
Biosensors 2014

Tuesday, 5 March 2013

Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from the latest issue:



► Fast and simultaneous quantitation of different classes of drugs in human plasma. ► Simple sample preparation step by protein precipitation. ► LC–MS/MS analysis with online sample clean up. ► Efficient tool for optimization of laboratory resource utilization.

Determination of gemcitabine and its metabolite in extracellular fluid of rat brain tumor by ultra performance liquid chromatography–tandem mass spectrometry using microdialysis sampling after intralesional chemotherapy   Original Research Article

Pages 10-19
Ying Sun, Daoquan Tang, Hui Chen, Fan Zhang, Bin Fan, Bei Zhang, Shaoying Fang, Qian Lu, Yaqin Wei, Jiale Yin, Xiaoxing Yin


► Gemcitabine is effective in the treatment of malignant gliomas. ► Gemcitabine's metabolite dFdU may contribute to gemcitabine's toxicity. ► A microdialysis technique with UPLC–MS/MS method was developed. ► All the validation data are within the required limits. ► The method was applied to determine gemcitabine and dFdU in rat tumor.

A liquid chromatography and tandem mass spectrometry method for the determination of potential biomarkers of cardiovascular disease   Original Research Article

Pages 20-29
Sylwia Magiera, Irena Baranowska, Jacek Kusa, Jacek Baranowski


► Development of HPLC–ESI-MS/MS method for the determination of potential biomarkers. ► Optimization of the MS/MS and chromatographic conditions. ► Solid-phase extraction procedure for human urine sample cleanup. ► This method could be useful in the detection of heart failure before symptoms of the disease.

Simultaneous determination of quinocetone and its major metabolites in chicken tissues by high-performance liquid chromatography tandem mass spectrometry   Original Research Article

Pages 30-37
Yan Yong, Yahong Liu, Limin He, Lixiao Xu, Yaping Zhang, Binghu Fang


► Quinocetone and its four metabolites. ► Chicken muscle, liver, kidney and fat tissues. ► A rapid and sensitive LC–MS/MS method is firstly developed. ► The elimination curves of quinocetone and its metabolites are obtained.



► Quantification of nucleoside and nucleotide analogs in plasma using HPLC–MS/MS. ► Zidovudine, abacavir, emtricitabine, lamivudine, tenofovir and ribavirin. ► Validation of the assay according to FDA guidelines.

A fast and reliable reversed phase high performance liquid chromatography method for simultaneous determination of selected anti-retroviral and lumefantrine in human plasma   Original Research Article

Pages 52-60
Betty Maganda, Olivier Heudi, Agnes Cortinovis, Franck Picard, Olivier Kretz, Omary Minzi


► Development and validation of fast, reproducible, and accurate LC-UV method. ► Simultaneous determination Nevirapine, Efavirenz and Lumefantrine in human plasma. ► The method is applicable to clinical samples from malaria and HIV patients. ► The method is easy to implement in laboratories with limited resources.

Sodium citrate and potassium phosphate as alternative adsorption buffers in hydrophobic and aromatic thiophilic chromatographic purification of plasmid DNA from neutralized lysate   Original Research Article

Pages 67-74
Nemailla Bonturi, Vanessa Soraia Cortez Oliveira Radke, Sônia Maria Alves Bueno, Sindélia Freitas, Adriano Rodrigues Azzoni, Everson Alves Miranda


► Alternative salts can be used in pDNA HIC and TAC as buffer systems. ► pDNA purification with HIC and TAC can be done directly with neutralized lysate. ► Mercaptopyrimidine agarose/sodium citrate system recovered 91.1% of the pDNA. ► Mercaptopyrimidine agarose/potassium phosphate system resulted in 98.8% pDNA purity.

  Short Communications

Rapid determination of sumatriptan in human plasma by ultra performance liquid chromatography–tandem mass spectrometry and its application to clinical pharmacokinetic study   

Pages 38-42
Jeong Ju Seo, Jeonghyeon Park, Min Ho Bae, Mi-sun Lim, Sook Jin Seong, Joomi Lee, Sung Min Park, Hae Won Lee, Young-Ran Yoon


► An U-HPLC–MS/MS analysis method for the quantification of sumatriptan was developed and validated using human plasma. ► This analysis method has been successfully validated in the 0.5–50ng/mL calibration range. ► The assay method precision was within 11.8%, and accuracy was between 97.4 and 102.9%. ► The developed and validated method was successfully applied to a pharmacokinetic study of sumatriptan.

Validation of an efficient LC-microdialysis method for gemifloxacin quantitation in lung, kidney and liver of rats   

Pages 62-66
Bibiana Verlindo de Araújo, João Victor Laureano, Lauren Dockhorn Grünspan, Teresa Dalla Costa, Leandro Tasso


► An efficient method for quantitation of unbound gemifloxacin concentrations is reported. ► Gemifloxacin was successfully determined in kidney, lung and liver of rats. ► Gemifloxacin unbound tissue concentrations were kidney>liver>lung.

Determination of ginsenoside Rc in rat plasma by LC–MS/MS and its application to a pharmacokinetic study   

Pages 75-78
Yang Chu, Hong-chao Zhang, Shu-ming Li, Jun-mei Wang, Xiang-yang Wang, Wei Li, Lan-lan Zhang, Xiao-hui Ma, Shui-ping Zhou, Yong-hong Zhu, Chang-xiao Liu


► It is the first analytical method (LC–MS/MS) for determining ginsenoside Rc in rat. ► It is the first report of bioavailability and pharmacokinetics of ginsenoside Rc. ► It provides the necessary information for the further investigation of ginseng.



No comments:

Post a Comment