Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

Journal of Chromatography B
http://rss.sciencedirect.com/publication/science/7220

Selected papers from the latest issue:

2.

Contribution to aroma characteristics of mutton process flavor from the enzymatic hydrolysate of sheep bone protein assessed by descriptive sensory analysis and gas chromatography olfactometry   Original Research Article Pages 1-8 Ping Zhan, Honglei Tian, Xiaoming Zhang, Liping Wang Highlights ► Sheep bone protein hydrolysates were used as precursor of mutton process flavor (MPF). ► Five sensory notes were selected to assess MPFs using descriptive sensory analysis. ► The corresponding volatile odor-active compounds were analyzed by GC–MS–O. ► We established the PLSR model to show the correlation between samples and others.

3.

Rapid and sensitive detection of Cronobacter spp. (previously Enterobacter sakazakii) in food by duplex PCR combined with capillary electrophoresis–laser-induced fluorescence detector   Original Research Article Pages 15-20 Jia Ruan, Ming Li, Ya-Pan Liu, Yuan-Qian Li, Yong-Xin Li Highlights ►Cronobacterspp. in food was determined by CE–LIF. ► Multiplex PCR parameters were optimized by CE in a rapid and intuitive way. ► Molecular technique combined with CE–LIF improved specificity and sensitivity. ► 120 infant food formula in China were tested for the presence ofCronobacterspp. ► This method was cheap, environmental friendly and suitable for routine analysis.

4.

Synthesis and characterization of a molecularly imprinted polymer for the determination of trace tributyltin in seawater and seafood by liquid chromatography–tandem mass spectroscopy   Original Research Article Pages 21-26 Shanshan Zhu, Futao Hu, Ting Yang, Ning Gan, Daodong Pan, Yuting Cao, Dazhen Wu Highlights ► Imprinted polymer with acrylamide as monomer for adsorbing tributyltin chloride. ► High retention capacity and selectivity for this adsorbent. ► Developing a new analysis approach by coupling this polymer to LC–MS/MS.

5.

Simultaneous quantification of 22R and 22S epimers of budesonide in human plasma by ultra-high-performance liquid chromatography–tandem mass spectrometry: Application in a stereoselective pharmacokinetic study   Original Research Article Pages 27-34 Youming Lu, Zuoming Sun, Yifan Zhang, Xiaoyan Chen, Dafang Zhong Highlights ► Simultaneously determine budesonide epimers in human plasma. ► Resolution and sensitivity of the analytes were highly improved by UHPLC system. ► The chromatography run time was significantly reduced to 7min.

6.

Simultaneous online SPE–HPLC–MS/MS analysis of docetaxel, temsirolimus and sirolimus in whole blood and human plasma   Original Research Article Pages 35-42 Alicia Navarrete, M. Paz Martínez-Alcázar, Ignacio Durán, Emiliano Calvo, Belén Valenzuela, Coral Barbas, Antonia García Highlights ► Online SPE–HPLC–MS/MS method for three antitumor drugs in a combined anticancer therapy. ► Harmonized sample treatment for plasma and whole blood samples. ► Analytical method validated. ► Real samples of plasma and blood from patients under oncologic therapy were measured and results are included.

7.

Simultaneous quantification of cefpodoxime proxetil and clavulanic acid in human plasma by LC–MS using solid phase extraction with application to pharmacokinetic studies   Original Research Article Pages 49-55 Anil Dubala, Janaki Sankarachari Krishnan Nagarajan, Chandran Sathish Vimal, Renjith George Highlights ► There was no LC–MS method reported for the simultaneous determination of CP and CA in biological samples. ► We developed and validated a rapid and sensitive LC–MS method for simultaneous estimation of CP and CA in human plasma. ► We evaluated the pharmacokinetic variables of tablet (containing CP 200mg and CA 125mg) after a single oral dose administration in twelve healthy human volunteers.

8.

Comparative validations of non-aqueous capillary electrophoresis and high-performance liquid chromatography methods for the simultaneous determination of histamine H2 receptor antagonists in human urine   Original Research Article Pages 56-63 Juan José Berzas Nevado, Gregorio Castañeda Peñalvo, Rosa María Rodríguez Dorado, Virginia Rodríguez Robledo Highlights ► Histamine H2 receptor antagonists are drugs used to block the action of histamine. ► A previously optimised electrophoretic method and HPLC standard method were used. ► Both analytical methods were compared using complete validation procedures. ► Both methods were used to the screening determination of the analytes in human urine.

9.

A simplified and completely automated workflow for regulated LC–MS/MS bioanalysis using cap-piercing direct sampling and evaporation-free solid phase extraction   Original Research Article Pages 64-74 Naiyu Zheng, Adela Buzescu, Stephanie Pasas-Farmer, Mark E. Arnold, Zheng Ouyang, Mohammed Jemal, Qianping Peng, Terry Van Vleet, Jianing Zeng Highlights ► The workflow for regulated bioanalysis is simplified and fully automated. ► Cap-piercing direct sampling and evaporation-free solid phase extraction are used. ► It eliminates manual intervention steps and potential risk of sample spillage. ► Good extraction recoveries, assay sensitivities and raggedness were achieved. ► It has been applied to three regulated bioanalytical assays for incurred samples.

10.

A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC)   Original Research Article Pages 75-80 Dennis J. Orton, Alan A. Doucette Highlights ► Temperature programming increases protein recovery above 90% in reversed phase LC. ► 214nm provides consistent response (by mass) across the proteome. ► LC–UV assay more accurately measures protein concentration versus colorimetric assays. ► LC–UV is applicable to SDS-containing samples, following detergent depletion.

11.

A rugged and accurate liquid chromatography–tandem mass spectrometry method for the determination of asunaprevir, an NS3 protease inhibitor, in plasma   Original Research Article Pages 81-86 Long Yuan, Hao Jiang, Zheng Ouyang, Yuan-Qing Xia, Jianing Zeng, Qianping Peng, Robert W. Lange, Yuzhong Deng, Mark E. Arnold, Anne-Françoise Aubry Highlights ► Developed and validated a rugged and accurate method for the determination of asunaprevir in plasma. ► A systematic method optimization strategy achieved optimized method and ensured assay quality. ► The validated method was successfully used to support pre-clinical toxicokinetic studies in different species.

12.

HPLC–UV method for measuring nicotinamide N-methyltransferase activity in biological samples: Evidence for substrate inhibition kinetics   Original Research Article Pages 87-95 Misha Patel, Muhammad M. Vasaya, Daniel Asker, Richard B. Parsons Highlights ► 1-Methylnicotinamide is produced from nicotinamide by nicotinamideN-methyltransferase. ► We describe a novel HPLC–UV method for detecting 1-methylnicotinamide in biological samples. ► Requires no sample preparation bar simple deprotonation. ► Linear over two orders of magnitude with LOD and LOQ of 0.05 and 0.15/100μL respectively. ► We show that nicotinamideN-methyltransferase demonstrates substrate inhibition kinetics.

Short Communications

13.

High throughput LC–MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma    Pages 9-14 Valluru Rajani Kumar, B. Phani Bhushana Reddy, B. Ravi Kumar, K. Sreekanth, Kilaru Naveen Babu Highlights ► LC–MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine. ► Increased sensitivity of 5ng/mL for zidovudine, lamivudine and 10ng/mL for nevirapine. ► Method utilizes highly selective solid extraction technique with simple sample preparation protocol.

14.

Multicomponent high-performance liquid chromatography/tandem mass spectrometry analysis of ten chemotherapeutic drugs in wipe samples    Pages 43-48 Shinichiro Maeda, Yoshihiro Miwa Highlights ► We developed a multicomponent analysis method for 10 common chemotherapy drugs. ► The lower limit of quantification for each drug was 5–50ng per wipe. ► Our procedure has sufficient sensitivity and is convenient for regular monitoring.