Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

Journal of Chromatography B
http://rss.sciencedirect.com/publication/science/7220

Selected papers from the latest issue:

1.

A validated HPLC–MS/MS assay for quantifying unstable pharmacologically active metabolites of clopidogrel in human plasma: Application to a clinical pharmacokinetic study    Available online 1 March 2013 Michael T. Furlong, Ishani Savant, Moucun Yuan, Laura Scott, William Mylott, Thomas Mariannino, Pathanjali Kadiyala, Vikram Roongta, Mark E. Arnold Highlights ► Assay to quantify four chemically reactive thiol metabolites of clopidogrel. ► Stabilization of the thiol group by alkylation with 2-bromo-3′-methoxyacetophenone. ► Separation of the four derivatized diastereomeric metabolites by HPLC. ► Application of the assay to a clinical pharmacokinetic study.

2.

Quantitative method for analysis of hydrocodone, hydromorphone and norhydrocodone in human plasma by liquid chromatography-tandem mass spectrometry    Available online 1 March 2013 Sandra Valtier, Robert L. Mueck, Vikhyat S. Bebarta Highlights ► We validated a method for quantitation of hydrocodone and metabolites in plasma. ► Samples were extracted using solid phase extraction and analyzed by LC-MS-MS. ► Linearity, precision, LOQ, and other method validation parameters were evaluated. ► The method provided good precision, accuracy and sensitivity.

3.

Combinatorial approach of LC-MS/MS and LC-TOF-MS for uncovering in vivo kinetics and biotransformation of ochratoxin A in rat   Original Research Article Available online 1 March 2013 Zheng Han, Zhiyong Zhao, Jianxin Shi, Yucai Liao, Zhihui Zhao, Dabing Zhang, Yongning Wu, Sarah De Saeger, Aibo Wu Highlights ► A combinatorial approach of LC-MS/MS and LC-TOF-MS was developed. ► The same conditions were used for the analysis in seven bio-matrices. ► The kinetics and distribution of OTA in rat were elucidated. ► Three typical metabolites of OTA were simultaneously identified.

4.

Validation of a novel extraction method for studying hexahydro-1,3,5-trinitro-1,3,5 triazine (RDX) biodegradation by ruminal microbiota   Original Research Article Available online 1 March 2013 J.G. Giarrizzo, L. Murty, D. Tanaree, K. Walker, A.M. Craig Highlights ► A novel RDX extraction method for small volume samples is proposed. ► Acceptable recoveries and % confidence were achieved in three different matrices. ► The proposed extraction method allowed detection of RDX degradation bi products by MS/MS. ► The method shows applicability in complex biological matrices.

5.

Reversed Phase High Performance Liquid Chromatography used for the physicochemical and thermodynamic characterization of piceatannol/β- cyclodextrin complex   Original Research Article Available online 27 February 2013 Hanane Messiad, Habiba Amira-Guebailia, Omar Houache Highlights ► The first attempt to study the complexation of Piceatannol with Beta-cyclodextrin. ► HPLC with UV-VIS detection was used for characterization of complex in solution. ► Decreasing retention times with increasing beta-cyclodextrin concentration confirmed the complex formation.

6.

Rapid determination of N-acetyl-4-aminophenol (Paracetamol) in urine by tandem mass spectrometry coupled with on-line clean-up by two dimensional turbulent flow/reversed phase liquid chromatography   Original Research Article Available online 27 February 2013 Hendrik Modick, André Schütze, Claudia Pälmke, Tobias Weiss, Thomas Brüning, Holger M. Koch Highlights ► Fast and reliable online LC/LC-MS/MS method for urinaryN-acetyl-4-aminophenol (NAAP). ► NAAP is both the major metabolite of aniline and a common pain reliever (Paracetamol). ► NAAP was quantifiable in all urine samples analysed. ► Urinary concentrations ranged from 8.7μg/L to 22100μg/L. ► Also non-Paracetamol users have a considerably body burden with NAAP.

7.

Simultaneous determination of opiates, methadone, buprenorphine and metabolites in human urine by superficially porous liquid chromatography tandem mass spectrometry   Original Research Article Available online 27 February 2013 Huei-Ru Lin, Chin-Lun Chen, Chieh-Liang Huang, Shao-Tsu Chen, Ahai-Chuang Lua Highlights ► Simultaneous determination of opiates and maintenance drugs by LC-MS/MS. ► The compound identification was fulfilled EU guideline. ► Drug monitoring ensures compliance and helps therapy.

8.

Liquid chromatography-tandem mass spectrometric assay for the mutated BRAF inhibitor dabrafenib in mouse plasma    Available online 27 February 2013 Rolf W. Sparidans, Selvi Durmus, Alfred H. Schinkel, Jan H.M. Schellens, Jos H. Beijnen Highlights ► The first validated bioanalytical assay for dabrafenib has been reported. ► The assay has successfully been validated in the 2-2000ng/ml range. ► The drug is sufficiently stable under all conditions relevant for the assay. ► First pharmacokinetics in male wild type FVB mice have reported.

9.

First HPLC-UV method for rapid and simultaneous quantification of phenobarbital, primidone, phenytoin, carbamazepine, carbamazepine-10,11-epoxide, 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine, lamotrigine, oxcarbazepine and licarbazepine in human plasma   Original Research Article Available online 27 February 2013 Ana Serralheiro, Gilberto Alves, Ana Fortuna, Marília Rocha, Amílcar Falcão Highlights ► Fast HPLC-UV method for the determination of antiepileptic drugs in human plasma. ► Simultaneous quantification of six antiepileptic drugs and their main metabolites. ► Sample pre-treatment is a combination of deproteinization followed by SPE. ► The method was fully validated according to international recommendations. ► This method was applied to real plasma samples from epileptic patients.

10.

SPE-UPLC-MS/MS method for sensitive and rapid determination of aripiprazole in human plasma to support a bioequivalence study    Available online 27 February 2013 Daxesh P. Patel, Primal Sharma, Mallika Sanyal, Pranav S. Shrivastav Highlights ► An improved UPLC-MS/MS method for determination of aripiprazole in human plasma. ► Highly sensitive and rapid compared to all existing methods in biological matrices. ► Practically free from endogenous matrix interference. ► Successful bioequivalence study in healthy Indian subjects. ► Reproducibility of study data is demonstrated by incurred sample reanalysis.

11.

Evaluation of different column chemistries for fast urinary metabolic profiling   Original Research Article Available online 26 February 2013 Dick-Paul Kloos, Henk Lingeman, Wilfried M.A. Niessen, André M. Deelder, Martin Giera, Oleg A. Mayboroda Highlights ► Comprehensive column chemistry evaluation for urinary metabolomics. ► Evaluation based on relevant analyte standards and pooled urine samples. ► Application of automated peak score evaluation and feature detection. ► Application to small sample set of urine specimen. ► Conclusion: superior behaviour of diol-HILIC material under specified conditions.

12.

Aptamer based extraction followed by electrospray ionization-ion mobility spectrometry for analysis of tetracycline in biological fluids   Original Research Article Available online 26 February 2013 Shabnam Najafi Aslipashaki, Taghi Khayamian, Zahra Hashemian Highlights ► A combination of an aptamer sorbent with the ion mobility spectrometry is introduced. ► This method was used for the determination of tetracycline in urine and plasma samples. ► The proposed method has a low detection limit and a wide dynamic range.

13.

Simultaneous determination of tramadol, o-desmethyltramadol and n-desmethyltramadol in human urine by gas chromatography-mass spectrometry    Available online 26 February 2013 Abdel-Aziz Y. El-Sayed, Khaled M. Mohamed, Ahmed Y. Nasser, Jennifer Button, David W. Holt Highlights ► Simultaneous determination of tramadol and its main metabolites using GC-MS was recommended. ► The presented extraction procedure offers a rapid way to isolate tramadol and its main metabolites from the urine matrix. ► The procedure was successfully applied for the analysis of tramadol and its main metabolites in urine samples. ► The procedure was easily incorporated into routine testing for a laboratory.

14.

Determination of N-methylcarbamate pesticides in vegetables by poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith microextraction coupled with high performance liquid chromatography   Original Research Article Available online 24 February 2013 Huihui Ma, Wei Feng, Miaomiao Tian, Qiong Jia Highlights ► PMME was developed for the determination ofN-methylcarbamate pesticides coupled with HPLC-DAD. ► A poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith was selected as the extraction medium for PMME. ► The method was applied to the determination ofN-methylcarbamate pesticides in vegetable samples.