Just Published: Journal of Chromatography A

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

Journal of Chromatography A

http://rss.sciencedirect.com/publication/science/5248

Selected papers from the latest issue:

2.

Analysis of trace contaminants in hot gas streams using time-weighted average solid-phase microextraction: Proof of concept   Original Research Article Pages 1-8 Patrick J. Woolcock, Jacek A. Koziel, Lingshuang Cai, Patrick A. Johnston, Robert C. Brown Highlights ► TWA-SPME method (retracted fiber) was developed for high temperature process gases. ► Experiments support theory of secondary boundary layer postulated by[33]. ► Trial application measuring syngas tar during gasification proves method functionality.

3.

Simultaneous determination of five estrogens and four androgens in water samples by online solid-phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry   Original Research Article Pages 9-18 Feng Guo, Qian Liu, Guang-bo Qu, Shan-jun Song, Jian-teng Sun, Jian-bo Shi, Gui-bin Jiang Highlights ► Estrogens and androgens were detected simultaneously by online SPE-HPLC/MS/MS. ► NG1 column showed the best extraction efficiency for nine analytes. ► Automated and efficient method were applied to analyze environmental water samples.

4.

Application of chromatography and mass spectrometry to the characterization of cobalt, copper, manganese and molybdenum in Morinda Citrifolia   Original Research Article Pages 19-25 Justyna Rybak, Lena Ruzik Highlights ► Investigation of elements (Cu, Co, Mn, Mo) content inMorinda Citrifolia– Noni juice. ► Speciation analysis of elements forms in buffer and enzymatic extracts. ► Investigation of elements bioavailability from Noni juice within vitromethod. ► Manganese is present in form of complexes with flavonoids and glycosides. ► Elements bioavailability from Noni juice depends on form – ligands complex.

Column Liquid Chromatography

5.

Probing the chiral separation mechanism and the absolute configuration of malathion, malaoxon and isomalathion enantiomers by chiral high performance liquid chromatography coupled with chiral detector–binding energy computations   Original Research Article Pages 26-31 Anping Zhang, Wenfeng Lai, Jianqiang Sun, Guixiang Hu, Weiping Liu Highlights ► Steric effect dominates chiral separation of malathion and malaoxon. ► Hydrogen bonding is a very important factor in chiral separation of isomalathion. ► The different notations are related for enantiomer of malathion and its metabolites. ► Binding energy computation can be used to predict enantiomeric resolution.

6.

A re-investigation of the Mark–Houwink–Sakurada parameters for cellulose in Cuen: A study based on size-exclusion chromatography combined with multi-angle light scattering and viscometry   Original Research Article Pages 32-37 Mürşide Kes, Bjørn E. Christensen Highlights ► Re-assessment of the MHS relation for cellulose in Cuen. ► Degradation correction of intrinsic viscosity in Cuen. ► Molecular weight distribution by SEC-MALLS in 0.5% DMAC/LiCl. ► DPn, DPwand properly defined DPvdetermined. ► (dn/dc)μdetermined.

7.

Analysis of hydroxycinnamic acids derivatives in calafate (Berberis microphylla G. Forst) berries by liquid chromatography with photodiode array and mass spectrometry detection   Original Research Article Pages 38-45 Antonieta Ruiz, Claudia Mardones, Carola Vergara, Isidro Hermosín-Gutiérrez, Dietrich von Baer, Patricio Hinrichsen, Roberto Rodriguez, Diego Arribillaga, Erwin Dominguez Highlights ► Calafate is a Patagonian berry rich in anthocyanins and other phenolic compounds. ► SPE with MCX column clean-up is essential to determine HCAD in calafate by HPLC-DAD. ► 20 HCAD were found in calafate, being 5-caffeoylquinic acid the most concentrated. ► Concentration of HCAD in calafate is higher than others widely consumed berries.

8.

Membrane assisted solvent extraction coupled with liquid chromatography tandem mass spectrometry applied to the analysis of alkylphenols in water samples   Original Research Article Pages 46-53 N. Salgueiro-González, I. Turnes-Carou, S. Muniategui-Lorenzo, P. López-Mahía, D. Prada-Rodríguez Highlights ► Analysis of alkylphenols in waters by MASE-LC-MS/MS. No previous works reported. ► Simplicity, fastness, selectivity and agreement with Green Chemistry principles. ► Lower MQL in compliance with Directive 2008/105/EC and proposal COM(2011)876 final. ► Fate and occurrence of APs in the seawater, surface and drinking water. ► Nonylphenol ubiquitous compound.

9.

“Mixed” anionic and non-ionic micellar liquid chromatography for high-speed radiometabolite analysis of positron emission tomography radioligands   Original Research Article Pages 54-59 Ryuji Nakao, Christer Halldin Highlights ► A mixed micellar LC method with anionic and non-ionic surfactants mobile phase. ► Addition of Triton X-100 on SDS eluents improved elution strength/peak efficiency. ► Simple isocratic elution without any treatment of plasma. ► Radiometabolite analysis was completed within 4min with high sensitivity.

10.

Overloading study of basic compounds with a positively charged C18 column in liquid chromatography   Original Research Article Pages 60-66 Chaoran Wang, Zhimou Guo, Zhen Long, Xiuli Zhang, Xinmiao Liang Highlights ► Considerable positive charge was proved on XCharge C18. ► Strikingly higher loadability of bases for XCharge C18 was obtained. ► The good loadability was closely related to the charge property.

11.

Design of experiments and multivariate analysis for evaluation of reversed-phase high-performance liquid chromatography with charged aerosol detection of sucrose caprate regioisomers   Original Research Article Pages 67-72 Aleksander Lie, Reinhard Wimmer, Lars Haastrup Pedersen Highlights ► A method for quantitative analysis of sucrose caprate regioisomers was developed. ► Charged aerosol detection provided mass-sensitivity in the range of 10–100ng. ► All regioisomers of sucrose caprate were successfully identified and assigned. ► Design of experiments were used in RP-HPLC analysis method development. ► Resolution deviation was defined for evaluation of regioisomer separation.

12.

Modeling of ion exchange expanded-bed chromatography for the purification of C-phycocyanin   Original Research Article Pages 73-78 Caroline Costa Moraes, Marcio A. Mazutti, Francisco Maugeri, Susana Juliano Kalil Highlights ► Mathematical modeling of the C-phycocyanin adsorption in expanded-bed columns. ► The proposed model satisfactorily fitted the experimental data. ► The increase in the initial resin height did not influence the process efficiency. ► Mathematical modeling is a promising tool to optimize chromatographic processes.

13.

Application and comparison of high performance liquid chromatography and high speed counter-current chromatography in enantioseparation of (±)-2-phenylpropionic acid   Original Research Article Pages 79-86 Shengqiang Tong, Ye Zheng, Jizhong Yan Highlights ► HPLC and HSCCC were successfully applied in enantioseparation of 2-phenylpropionic acid. ► The mechanism of cyclodextrin inclusion complex was investigated by analytical HPLC. ► The two-phase solvent system was selected for enantioseparation by HSCCC. ► HSCCC separation conditions were optimized by liquid–liquid extraction experiments. ► The preparative HPLC and HSCCC were compared in chiral separation.

14.

A multimodal histamine ligand for chromatographic purification of plasmid DNA   Original Research Article Pages 87-93 Urh Černigoj, Urška Vidic, Miloš Barut, Aleš Podgornik, Matjaž Peterka, Aleš Štrancar Highlights ► A new chromatographic method for the separation of pDNA isoforms was developed. ► The method employs a multimodal histamine ligand on a monolith. ► Hydrophobic and electrostatic interactions are expressed simultaneously. ► The balance of interaction is determined by the titration state of the histamine. ► Both plasmid isoforms were proven to be selectively eluted on an analytical as well as on preparative scale.

15.

Comparison of two serially coupled column systems and optimization software in isocratic liquid chromatography for resolving complex mixtures   Original Research Article Pages 94-105 C. Ortiz-Bolsico, J.R. Torres-Lapasió, M.J. Ruiz-Ángel, M.C. García-Álvarez-Coque Highlights ► Comparison of two approaches for serially coupling columns. ► Advantages and drawbacks of the column coupling systems. ► Discussion on the factors that improve the reliability of the predictions. ► Guidelines to develop software accounting peak retention and shape. ► Isocratic optimization of the separation of a set of 15 sulphonamides.

Column Liquid Chromatography and Gas Chromatography

16.

Programmed temperature vaporizing injector to filter off disturbing high boiling and involatile material for on-line high performance liquid chromatography gas chromatography with on-column transfer   Original Research Article Pages 106-114 Maurus Biedermann, Koni Grob Highlights ► PTV injector to protect the GC column in on-line HPLC–GC. ► Visual experiments for the investigation of the process. ► Working instructions for best performance.

Gas Chromatography

17.

Evaluation of different derivatisation approaches for gas chromatographic–mass spectrometric analysis of carbohydrates in complex matrices of biological and synthetic origin   Original Research Article Pages 115-126 M. Becker, T. Zweckmair, A. Forneck, T. Rosenau, A. Potthast, F. Liebner Highlights ► Six different single- and two-step carbohydrate derivatisation approaches compared. ► Reference compounds comprised 8 monosaccharides (C5–C6), GA and DHA. ► Ethoximation–trimethylsilylation was found to be superior to other approaches. ► Analysis of carbohydrate-rich matrices of different origin is possible. ► Calculation of a novel matrix impact value is proposed to evaluate matrix effects.

Supercritical and Subcritical Fluid Chromatography

18.

Effects of column back pressure on supercritical fluid chromatography separations of enantiomers using binary mobile phases on 10 chiral stationary phases   Original Research Article Pages 127-134 Chunlei Wang, Yingru Zhang Highlights ► We systematically study pressure effects on chiral SFC separations. ► We discuss in detail the pressure effects ont0,k,α,NandRs. ► We observe, for the first time, a linear relationship between dk/dPandkitself. ► Based on 48 enantio-separations, theαis generally independent on back pressure. ► Resolution decreases at higher back pressure due to retention and efficiency loss.

Planar Chromatography

19.

A new high-performance thin layer chromatography-based assay of detergents and surfactants commonly used in membrane protein studies   Original Research Article Pages 135-141 Laurie-Anne Barret, Ange Polidori, Françoise Bonneté, Pierre Bernard-Savary, Colette Jungas Highlights ► HPTLC is proposed as a method for improved quantification of detergents. ► The quantification range is between 0.1 and 1.4μg in membrane protein solutions. ► The routine detergent assay is performed without prior treatment. ► Detergent exchange is quantified via a single procedure.

Electrophoresis and Electrochromatography

20.

Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication   Original Research Article Pages 142-147 Petr Kubáň, František Foret, Robert Bocek Highlights ► A new method for rapid analysis of formic acid by CE with C4D was developed. ► Formic acid is analyzed in blood serum samples in less than 2min. ► No sample preparation except dilution with DI water is required. ► The method provides a simple diagnostic test for methanol intoxication. ► The method was successfully tested on patients with acute methanol intoxication.

21.

Fast and interference-free determination of glyphosate and glufosinate residues through electrophoresis in disposable microfluidic chips   Original Research Article Pages 148-154 Xuan Wei, Xiaotong Gao, Lei Zhao, Xianglu Peng, Lei Zhou, Jing Wang, Qiaosheng Pu Graphical abstract A fast and interference-free method based on microchip electrophoresis with laser induced fluorescence detection was established for the determination of trace amount of glyphosate and glufosinate residues in agricultural products. Highlights ► Microchip electrophoresis was adopted for analysis of glyphosate and glufosinate. ► Easy-to-make low-cost plastic microchips and hardwares were utilized. ► Anodic separation was used to get rid of potential interferences. ► The method was fast, sensitive and excellent in interference tolerance. ► The method was applied in the analysis of real samples with complicated matrices.

Short Communications

Column Liquid Chromatography

22.

Investigation of peptoid chiral stationary phases varied in absolute configuration    Pages 155-159 Haibo Wu, Kuiyong Li, Hui Yu, Yanxiong Ke, Xinmiao Liang Highlights ► Peptoid CSPs varied in absolute configuration were investigated. ► Homogeneous chiral sides in peptoid selector were proved unnecessary. ► CSP with heterogeneous configuration form exhibited specific selectivity. ► The chiral residue next to the terminus was important for chiral recognition. ► Longer peptoid chain was not an essential condition for enantiorecognition.

23.

Separation of piracetam derivatives on polysaccharide-based chiral stationary phases    Pages 160-165 H. Kažoka, O. Koliškina, G. Veinberg, M. Vorona Highlights ► Enantioseparation of two chiral drugs was examined. ► High chiral recognition ability of column Lux Amylose-2 was observed. ► The influence of an alcoholic modifier on enantioseparation was studied.