A new issue of this journal has just
been published. To see abstracts of the papers it contains (with links through
to the full papers) click here:
Selected
papers from the latest issue:
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2.
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Innovative
biocompatible nanospheres as biomimetic platform for electrochemical glucose
biosensor Original Research Article
Pages 1-5 Wenbo Zhao, Yalong Ni, Qinshu Zhu, Rongjin Fu, Xiaohua Huang, Jian Shen
Highlights
►
Innovative SiO2–PA NPs were prepared by reverse microemulsion and
electrostatic binding method. ► Biomimetic surface provided by SiO2–PA NPs
could preserve bioactivity of GOx. ► The GOxbiosensor modified by SiO2–PA NPs
exhibited an excellent electrochemical behavior. |
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3.
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A
“turn-off” SERS-based detection platform for ultrasensitive detection of
thrombin based on enzymatic assays
Original Research
Article
Pages 10-15 Zitong Wu, Yizhen Liu, Xiaodong Zhou, Aiguo Shen, Jiming Hu
Highlights
►
“Turn-off” SERS-based enzymatic assay is newly proposed. ► The method works
on catalytic property of thrombin to cleave multi-arginine peptides. ►
Ultrasensitive detection of target was achieved together with potential of
use in complex matrix. |
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4.
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Immobilization
of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes
of opposite charges for the development of the reusable fluorescent
Cu2+-sensor Original Research Article
Pages 16-20 Lei Su, Tong Shu, Zongwei Wang, Jingya Cheng, Feng Xue, Chenzhong Li, Xueji Zhang
Highlights
► The
BSA-protected GNCs were modified with polyelectrolytes of opposite charges. ►
Bare glass slides were modified with the prepared complexes of
protein–polyelectrolytes. ► A reusable fluorescence sensor for copper ion was
proposed. |
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5.
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Interaction
of anticancer drug methotrexate with DNA analyzed by electrochemical and
spectroscopic methods Original Research Article
Pages 21-26 Bushra Rafique, Ahmad M. Khalid, Kalsoom Akhtar, Abdul Jabbar
Highlights
► An
electrochemical DNA biosensor was constructed by immobilizing the DNA to the
surface of glassy carbon electrode (GCE). ► This electrode was used to
determine the binding of methotraxate drug with DNA. ► Determination of
bonding was done by using constant current potentiometric technique,
UV–Visible and FTIR spectroscopy. ► Results demonstrated the intercalative
binding between DNA and methotraxate. |
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6.
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Local
detection of mechanically induced ATP release from bone cells with ATP
microbiosensors Original Research Article
Pages 27-33 Elena Hecht, Astrid Liedert, Anita Ignatius, Boris Mizaikoff, Christine Kranz
Highlights
►
Miniaturized ATP biosensors. ► Scanning electrochemical microscopy combined
with a cell stretching device. ► Mechanically induced ATP release at MC3T3-E1
osteoblastic cells. |
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7.
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Fabrication
of a new electrochemical sensor based on a new nano-molecularly imprinted
polymer for highly selective and sensitive determination of tramadol in human
urine samples Original Research Article
Pages 34-40 Abbas Afkhami, Hamed Ghaedi, Tayyebeh Madrakian, Mazaher Ahmadi, Hedye Mahmood-Kashani
Highlights
► A new
nano-molecularly imprinted polymer bead was synthesized. ► The prepared
nano-polymer bead was characterized by different methods. ► The bead was used
to the fabrication of a chemically modified CPE. ► The electrode was used to
the rapid and selective determination of tramadol. |
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8.
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Horseradish
peroxidase-catalyzed synthesis of poly(thiophene-3-boronic acid)
biocomposites for mono-/bi-enzyme immobilization and amperometric biosensing Original Research Article
Pages 41-47 Yi Huang, Wen Wang, Zou Li, Xiaoli Qin, Lijuan Bu, Zhiyong Tang, Yingchun Fu, Ming Ma, Qingji Xie, Shouzhuo Yao, Jiming Hu
Highlights
►
Preparation of mono-/bi-enzyme biocomposites by enzymatic polymerization. ►
Boronic acid polymer as a biocompatible matrix for enzyme immobilization. ►
High-performance biosensing of H2O2and glucose. |
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9.
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A
novel enzyme-free and label-free fluorescence aptasensor for amplified
detection of adenosine
Original Research
Article
Pages 52-56 Bo Fu, Jichao Cao, Wei Jiang, Lei Wang
Highlights
► A novel
fluorescence amplified aptasensor was developed. ► The aptasensor was based
on target-catalyzed self-hairpin assembly. ► The aptasensor was enzyme-free
and label-free, making it simple and inexpensive. ► The limit of detection of
the aptasensor for adenosine was 6μM. ► This strategy provided a simple,
rapid and high selective versatile platform. |
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10.
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A
highly sensitive label-free electrochemical aptasensor for interferon-gamma
detection based on graphene controlled assembly and nuclease
cleavage-assisted target recycling amplification Original Research Article
Pages 57-63 Genping Yan, Yonghong Wang, Xiaoxiao He, Kemin Wang, Jinquan Liu, Yudan Du
Highlights
► We
develop an electrochemical aptasensor for IFN-γ detection. ► The detection
method is based on graphene and nuclease cleavage amplification. ► Signal
transduction and amplification of graphene on the MHA/SAM electrode. ► It is
a label-free, highly sensitive strategy for the detection of IFN-γ. ► The
assay has good response in complicated cell media. |
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11.
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Construction
of an amperometric bilirubin biosensor based on covalent immobilization of
bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid
film Original Research Article
Pages 64-69 Bhawna Batra, Suman Lata, Sunny, J.S. Rana, Chandra Shekhar Pundir
Highlights
►
Constructed a BOx/SiO2@ZrONPs/CHIT/Au hybrid film. ► Fabricated an improved
amperometric bilirubin biosensor based on this film. ► Biosensor had a
detection limit of 0.1nM and linear range of 0.02–250μM. ► Employed for
bilirubin determination in different serum samples. |
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12.
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Electrochemical
sensor using neomycin-imprinted film as recognition element based on
chitosan-silver nanoparticles/graphene-multiwalled carbon nanotubes
composites modified electrode
Original Research
Article
Pages 70-76 Wenjing Lian, Su Liu, Jinghua Yu, Jie Li, Min Cui, Wei Xu, Jiadong Huang
Highlights
► A
selective electrochemical molecularly imprinted sensor for the detection of
neomycin was developed. ► CS-SNP and GR-MWCNTs nanocomposites were used to
improve the sensitivity of the sensor. ► MIPs were synthesized by
electropolymerization using neomycin as the template, and pyrrole as the
monomer. ► The sensor showed high selectivity, excellent stability and good
reproducibility for the determination of neomycin. ► The developed sensor was
successfully used for neomycin determination in real spiked samples. |
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13.
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Integration
of gold nanoparticles in PDMS microfluidics for lab-on-a-chip plasmonic
biosensing of growth hormones
Original Research
Article
Pages 77-84 Hamid SadAbadi, Simona Badilescu, Muthukumaran Packirisamy, Rolf Wüthrich
Highlights
►
Anin-situmethod for integration of gold NP in a PDMS microfluidic chip is
proposed. ► The kinetics is fully studied and the activation energy is
estimated to be 30kJ/mol. ► The reaction in microchannel is found to be
slower than at the macro-scale. ► The size uniformity of GNPs is found to be
considerably improved in the micro-environment. ► A detection limit of 4ng/mL
for bovine GH is obtained for the proposed biosensor. |
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14.
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Porous
silicon optical microcavity biosensor on silicon-on-insulator wafer for
sensitive DNA detection
Original Research
Article
Pages 89-94 Hongyan Zhang, Zhenhong Jia, Xiaoyi Lv, Jun Zhou, Liangliang Chen, Rongxia Liu, Ji Ma
Highlights
► A
sensitive label-free PSM biosensor on SOI wafer was fabricated by
electrochemical etching. ► By simulations and experiments, we optimized PSM
biosensor with a high Q value and a high sensitivity. ► This biosensor was
used for DNA detection and red shift shows a good linear relationship with
DNA. ► This optical PSM on SOI could be a great potential for biochips based
on integrated optical devices. |
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15.
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In
situ amplification signaling-based autonomous aptameric machine for the
sensitive fluorescence detection ofcocaine Original Research Article
Pages 95-100 Su-Jin Xie, Hui Zhou, Dengyou Liu, Guo-Li Shen, Ruqin Yu, Zai-Sheng Wu
Highlights
► In situ
amplification signaling-based aptameric machine has been developed. ►
Discovering operation behavior of scission/replication-based autonomous
machine. ► The developed aptameric machine exhibits substantially improved
assay characteristics. |
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16.
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Multiplex
electrochemiluminescence immunoassay of two tumor markers using multicolor
quantum dots as labels and graphene asconductingbridge Original Research Article
Pages 101-107 Zhiyong Guo, Tingting Hao, Shuping Du, Beibei Chen, Zebo Wang, Xing Li, Sui Wang
Highlights
► A
multiplex ECL immunoassay of two tumor markers was proposed. ► Multicolor QDs
were labeled to obtain distinguishable ECL signals. ► G was used as a
conducting bridge, improving the ECL intensity about 30-folds. ► LOD for AFP
and CEA was about 0.4fg/mL. |
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17.
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Nanogold-enhanced
graphene nanosheets as multienzyme assembly for sensitive detection of
low-abundanceproteins Original Research Article
Pages 108-114 Huafeng Chen, Zhuangqiang Gao, Yuling Cui, Guonan Chen, Dianping Tang
Highlights
► We
introduce a new electrochemical immunoassay protocol for sensitive detection
of low-abundance proteins. ► Using graphene-carried
poly(o-phenylenediamine)/gold hybrid nanosheets as molecular tags. ► Design
of redox-active nanolabels. |
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18.
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Detection
of exosomal biomarker by electric field-induced release and measurement
(EFIRM) Original Research Article
Pages 115-121 Fang Wei, Jieping Yang, David T.W. Wong
Highlights
► EFIRM
technique has been established for measuring biomolecules in exosomes. ► It
uses a non-uniform electrical field to release the RNA/proteins from exosome.
► We confirmedin vivoH460-CD63-GFP exosomes transported to blood and saliva.
► It provides an on-site detection of distal tumor-shed biomarkers in
exosome. |
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19.
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Construction
and evaluation of two biosensors based on yeast transcriptional response to
genotoxic chemicals Original Research Article
Pages 138-145 Ting Wei, Chao Zhang, Xin Xu, Michelle Hanna, Xiaohua Zhang, Yan Wang, Heping Dai, Wei Xiao
Highlights
►
SelectedyEGFPas a reporter. ► Chose and compared theRNR3 andHUG1 promoters as
sensors. ► Constructed a yeast septuple mutant with seven genes deleted to
enhance the detection sensitivity. ► The resulting biosensors are much more
sensitive than previously reported similar systems. |
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20.
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Simultaneous
electrochemical determination of guanosine and adenosine with graphene–ZrO2
nanocomposite modified carbon ionic liquid electrode Original Research Article
Pages 146-151 Wei Sun, Xiuzhen Wang, Xiaohuan Sun, Ying Deng, Jun Liu, Bingxin Lei, Zhenfan Sun
Highlights
► Graphene
and ZrO2nanoparticles based nanocomposite were used for the electrode
modification. ► Adenosine and guanosine were simultaneous detected by the
modified electrode. ► The proposed method was applied to the human urine
samples with satisfactory results. |
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21.
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Biomimetic
sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for
nitric oxide measurement in the brain
Original Research
Article
Pages 152-159 Ricardo M. Santos, Marcelo S. Rodrigues, João Laranjinha, Rui M. Barbosa
Highlights
► A
microsensor for nitric oxide (NO) is designed based on a novel nanocomposite
film. ► Cyclic voltammetry shows direct electron transfer from hemin. ► The
electrocatalytic reduction of NO provides high sensitivity and selectivity. ►
The microsensor is able to resolve NO and oxygen peaks by square wave
voltammmetry. ► Measurement of exogenously applied NO in vivo in the rat
brain is demonstrated. |
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22.
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Direct
label free ultrasensitive impedimetric DNA biosensor using dendrimer
functionalized GaN nanowires
Original Research
Article
Pages 164-170 Prasana Sahoo, Sumathi Suresh, Sandip Dhara, Garima Saini, S. Rangarajan, A.K. Tyagi
Highlights
► Nanowire
based in-situ detection of DNA hybridization is demonstrated. ► An ultra low
detection limit down toaMtarget DNA solution has been achieved. ► The
selectivity of the sensor probe is shown by monitoring the sensor response
from other non-specific target DNA. ► Change in the interfacial polarization
resistance serves the basis of the present DNA biosensor. |
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23.
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A
label-free microRNA biosensor based on DNAzyme-catalyzed and microRNA-guided
formation of a thin insulating polymer film Original Research Article
Pages 171-176 Wei Shen, Huimin Deng, Yuqian Ren, Zhiqiang Gao
Highlights
► A
PCR-free and label-free microRNA biosensor is developed. ► The accumulative
nature of the deposition of a thin insulating film drastically improves the
detectability of the biosensor. ► The amplifier—DNAzyme was found to be much
more tolerant than natural enzymes in terms of reaction conditions. ►
Attempts were made in detecting miRNAs in total RNA extracted from cancer
cells. |
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24.
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Label-free
electrogenerated chemiluminescence biosensing method for trace bleomycin
detection based on a Ru(phen)32+–hairpin DNA composite film electrode Original Research Article
Pages 177-182 Yan Li, Cancan Huang, Jianbin Zheng, Honglan Qi, Wei Cao, Yinmao Wei
Highlights
►
Label-free ECL DNA-based biosensing method for bleomycin was developed. ► The
ECL biosensing method is based on Fe(II)·BLMs-mediated DNA strand scission. ►
The biosensing method showed high sensitivity and selectivity. |
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25.
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An
electrochemical biosensor for ascorbic acid based on carbon-supported
PdNinanoparticles Original Research Article
Pages 183-190 Xin Zhang, Yang Cao, Sha Yu, Fengchun Yang, Pinxian Xi
Highlights
►
Carbon-supported PdNi nanoparticles (PdNi/C) prepared. ► A novel synthetic
route for PdNi/C preparation. ► The performance was better than Pd/C because
of PdNi synergistic effects. ► PdNi/C was employed for ascorbic acid
determination. ► High selectivity, good stability, wide linear range and high
sensitivity. |
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26.
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One-step
fabrication of sub-10-nm plasmonic nanogaps for reliable SERS sensing of
microorganisms Original Research Article
Pages 191-197 Jing Chen, Gaowu Qin, Jiansheng Wang, Jiangyu Yu, Bo Shen, Song Li, Yuping Ren, Liang Zuo, Wen Shen, Biswajit Das
Highlights
► SERS
substrates with high-density of sub-10-nm nanogaps were fabricated by high
pressure sputtering in one-step. ► We demonstrate the reliability and
reproducibility of this clean substrate which has no remaining chemicals. ►
Excellent SERS spectra of four kinds of microorganisms were acquired. ► By
introducing sub-10-nm nanogaps, the SERS substrate enhancement factor is
improved by 103times. ► Piecewise-linear relationship exists between the SERS
intensity and the rhodamine 6G concentration. |
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27.
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Label-free
impedimetric immunosensors for psoriasin—Increased reproducibility and
sensitivity using an automated dispensing system Original Research Article
Pages 198-203 Timothy R.J. Holford, Joanne L. Holmes, Stuart D. Collyer, Frank Davis, Séamus P.J. Higson
Highlights
►
Developed and optimised a label-free immunosensor for psoriasin. ► Antibodies
to psoriasin immobilised using avidin–biotin or electrostatic interactions. ►
Both manual and automated (BioDot AD3200 micro-dispenser) deposition used. ►
Affinity immobilised sensors display much higher sensitivity than
electrostatic. ► BioDot produced sensors display higher sensitivity and lower
sample-to-sample variation. Levels of psoriasin from 250–10,000pgml−1can be
clearly determined. |
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28.
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Quantum
dots-based fluorescent probes for turn-on and turn-off sensing of
butyrylcholinesterase Original Research Article
Pages 204-209 Zhenzhen Chen, Xiangling Ren, Xianwei Meng, Longfei Tan, Dong Chen, Fangqiong Tang
Highlights
►
Biosensor was composed of QDs and enzymes, without any complex processes. ►
BChE detected by one step enzyme reaction for the first time. ► We studied
the detection mechanisms of turn-on and turn-off detection system. ► We
demonstrated the application in real serum samples. ► This work presents a
feasible approach for detecting some kinds of substrate. |
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29.
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A
nanoporous membrane-based impedimetric immunosensor for label-free detection
of pathogenic bacteria in whole milk
Original Research
Article
Pages 210-215 Cho-Kyung Joung, Han-Nah Kim, Min-Cheol Lim, Tae-Joon Jeon, Hae-Yeong Kim, Young-Rok Kim
Highlights
►
Nanoporous membrane-based impedimetric immunosensor for pathogen detection in
milk. ► Sensitivity and selectivity greatly enhanced by hyaluronic acid
interface. ► Detection limit of <102cfu/ml forE. coliO157 in whole milk. |
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30.
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A
sensor tip based on carbon nanotube-ink printed electrode for the dengue virus
NS1 protein Original Research Article
Pages 216-221 Ana Carolina M.S. Dias, Sérgio L.R. Gomes-Filho, Mízia M.S. Silva, Rosa F. Dutra
Highlights
► A
disposable immunosensor based on carbon nanotube-screen printed electrode was
developed for NS1 protein of the dengue virus. ► Anti-NS1 antibodies were
covalently bound to the CNT-tip surface by ethylenediamine film. ► Good limit
of detection (12ngmL−1) was obtained and serum samples were analyzed. |
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31.
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Rapid,
quantitative, reverse transcription PCR in a polymer microfluidicchip Original Research Article
Pages 222-228 D. Curtis Saunders, Gregory L. Holst, Christopher R. Phaneuf, Nikita Pak, Matthew Marchese, Nicholas Sondej, Michael McKinnon, Craig R. Forest
Highlights
A
user-friendly instrument for microfluidic quantitative PCR and RT-PCR is
reported. ► Plastic, disposable microchips were used to contain 1μl reaction
volumes. ► Precision mechanical alignment and thermal stability allow
repeatable open-loop control. ► Quantitative PCR and RT-PCR with viral DNA
and GAPDH RNA were demonstrated. ► Electrophoretic end-point detection and
melting curve analysis confirmed amplifications. |
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32.
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Selective
virus detection in complex sample matrices with photonic crystal optical
cavities Original Research Article
Pages 229-234 Sudeshna Pal, Amrita R. Yadav, Mark A. Lifson, James E. Baker, Philippe M. Fauchet, Benjamin L. Miller
Highlights
► 2-D
Photonic Crystals are optical sensors with an exceptionally small sensing
volume. ► We conducted FDTD calculations that suggest single virus detection
is possible. ► Detection of human papillomavirus virus-like particles (VLPs)
was demonstrated. ► High selectivity was obtained in buffer and in serum. |
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33.
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Electrochemical
immunosensor for ultrasensitive detection of microcystin-LR based on
graphene–gold nanocomposite/functional conducting polymer/gold
nanoparticle/ionic liquid composite film with electrodeposition Original Research Article
Pages 235-240 Li Ruiyi, Xia Qianfang, Li Zaijun, Sun Xiulan, Liu Junkang
Highlights
► Proposed
strategy to fabricate the graphene–gold nanocomposite and sensor is green and
controllable. ► Graphene–gold nanocomposite remarkably enhance the electron
transfer rate. ► The antibody was covalently immobilized on the electrode
using functional conducing polymer. ► The ionic liquid improves
biocompatibility of the microenvironment for the antibody. ► The sensor for
microcystin-LR offers the best sensitivity and stability up to now. |
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34.
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Integrated
biochip for label-free and real-time detection of DNA amplification by
contactless impedance measurements based on interdigitated electrodes Original Research Article
Pages 241-247 Xinxin Fang, Qinghui Jin, Fengxiang Jing, Huanqian Zhang, Feng Zhang, Hongju Mao, Baojian Xu, Jianlong Zhao
Highlights
►
Integrated biochip for impedance detection of DNA amplification in real-time
is reported. ► The chip includes temperature controlling electrodes and
interdigitated EC electrodes. ► The biochip exhibits rapid detection
efficiency and high sensitivity for SDA. ► The biochip is developed first to
monitor RCA in real-time. |
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Short Communications
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35.
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Double
strand DNA-templated copper nanoparticle as a novel fluorescence indicator
for label-free detection of polynucleotide kinase activity
Pages 6-9 Liangliang Zhang, Jingjin Zhao, Hua Zhang, Jianhui Jiang, Ruqin Yu
Highlights
► A simple
PNK activity assay using dsDNA-templated CuNPs as a fluorescent indicator was
presented. ► Any other additional modifications of DNA substrate or complex
design were not required for this method. ► PNK activity was sensitively and
selectively detected. ► In complex biological samples, this method exhibited
a good assay performance. |
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36.
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A
novel photoelectrochemical sensor based on PPIX-functionalized WO3–rGO
nanohybrid-decorated ITO electrode for detecting cysteine
Pages 48-51 Bing Sun, Kun Zhang, Lijian Chen, Lintong Guo, Shiyun Ai
Graphical abstract
Highlights
► A
universal PEC sensing platform was fabricated based on a ternary composition
of PPIX–WO3–rGO. ► The multiple electron transfer system of PPIX–WO3–rGO
obtained enhanced photocurrent. ► A highly sensitive and selective biosensor
for PEC detection of cysteine developed. ► The proposed PEC sensor opened up
a new avenue to the application of WO3nanostructures. |
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37.
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Sensitive
amperometric biosensor for phenolic compounds based on graphene–silk
peptide/tyrosinase composite nanointerface
Pages 85-88 Ying Qu, Ming Ma, Zhengguo Wang, Guoqing Zhan, Buhai Li, Xian Wang, Huaifang Fang, Huijuan Zhang, Chunya Li
Highlights
►Silk
peptide functionalized graphene nanosheets were synthesized and fully
characterized;►Graphene–silk peptide–tyrosinase/glassy carbon electrodes
(Gr–SP–Tyr/GCE) were fabricated;►Electrochemical behaviors of phenolic
compounds at Gr–SP–Tyr/GCE were investigated;►Sensitive amperometric method
for phenolic compounds was developed;►Practical application of the biosensor
was demonstrated. |
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38.
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Development
of a novel electrochemical sensor using pheochromocytoma cells and its
assessment of acrylamide cytotoxicity
Pages 122-126 Xiulan Sun, Jian Ji, Donglei Jiang, Xiaowei Li, Yinzhi Zhang, Zaijun Li, Yongning Wu
Highlights
► A novel
living cell-based electrochemical sensor was fabricated. ► Graphene–gold
nanocomposite as signal amplification was used for living cell
immobilization. ► The electrochemical sensor was successfully used for
monitoring toxic effects of acrylamide on PC-12 cells. |
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39.
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A
one-pot ‘green’ synthesis of Pd-decorated PEDOT nanospheres for nonenzymatic
hydrogen peroxide sensing
Pages 127-131 Fengxing Jiang, Ruirui Yue, Yukou Du, Jingkun Xu, Ping Yang
Graphical abstract
Highlights
► One-pot
facile synthesis of Pd-decorated homogeneous PEDOT nanospheres. ► No any
surfactant and template to be used. ► 3D porous nanostructure for Pd/PEDOT
nanospheres. ► Well-dispersed Pd nanoparticles on PEDOT nanospheres. ► High
sensitivity and low detection limit for Pd/PEDOT as H2O2sensor. |
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40.
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CdTe
nanobioprobe based optoelectrochemical immunodetection of diabetic marker
HbA1c
Pages 132-135 Adity Chopra, Satish Tuteja, Naresh Sachdeva, K.K. Bhasin, Vijayender Bhalla, C. Raman Suri
Highlights
► Highly
luminescent aqueous CdTe quantum dots synthesized. ► Quantum dots conjugated
with specific antibody used as nanobioprobe. ► Dual fluorescence cum
electrochemical format for HbA1c detection. ► Correlation coefficient of 96%
for electrochemical detection with HPLC. ► Highly reproducible and promising
HbA1c detection platform. |
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41.
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Direct
energy conversion from xylose using xylose dehydrogenase surface displayed
bacteria based enzymatic biofuel cell
Pages 160-163 Lin Xia, Bo Liang, Liang Li, Xiangjiang Tang, Ilaria Palchetti, Marco Mascini, Aihua Liu
Highlights
► First
report on the direct energy conversion from xylose using xylose dehydrogenase
displayed bacteria based biofuel cell. ► Maximum power density of the cell up
to 63μWcm−2. ► The open-circuit potential of the cell up to 0.58V. |
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Book Review
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42.
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