World Congress on Biosensors 2014

World Congress on Biosensors 2014
Biosensors 2014

Monday, 8 April 2013

Just Published: Biosensors & Bioelectronics

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from the latest issue:

Innovative biocompatible nanospheres as biomimetic platform for electrochemical glucose biosensor   Original Research Article

Pages 1-5
Wenbo Zhao, Yalong Ni, Qinshu Zhu, Rongjin Fu, Xiaohua Huang, Jian Shen


► Innovative SiO2–PA NPs were prepared by reverse microemulsion and electrostatic binding method. ► Biomimetic surface provided by SiO2–PA NPs could preserve bioactivity of GOx. ► The GOxbiosensor modified by SiO2–PA NPs exhibited an excellent electrochemical behavior.

A “turn-off” SERS-based detection platform for ultrasensitive detection of thrombin based on enzymatic assays   Original Research Article

Pages 10-15
Zitong Wu, Yizhen Liu, Xiaodong Zhou, Aiguo Shen, Jiming Hu


► “Turn-off” SERS-based enzymatic assay is newly proposed. ► The method works on catalytic property of thrombin to cleave multi-arginine peptides. ► Ultrasensitive detection of target was achieved together with potential of use in complex matrix.

Immobilization of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes of opposite charges for the development of the reusable fluorescent Cu2+-sensor   Original Research Article

Pages 16-20
Lei Su, Tong Shu, Zongwei Wang, Jingya Cheng, Feng Xue, Chenzhong Li, Xueji Zhang


► The BSA-protected GNCs were modified with polyelectrolytes of opposite charges. ► Bare glass slides were modified with the prepared complexes of protein–polyelectrolytes. ► A reusable fluorescence sensor for copper ion was proposed.

Interaction of anticancer drug methotrexate with DNA analyzed by electrochemical and spectroscopic methods   Original Research Article

Pages 21-26
Bushra Rafique, Ahmad M. Khalid, Kalsoom Akhtar, Abdul Jabbar


► An electrochemical DNA biosensor was constructed by immobilizing the DNA to the surface of glassy carbon electrode (GCE). ► This electrode was used to determine the binding of methotraxate drug with DNA. ► Determination of bonding was done by using constant current potentiometric technique, UV–Visible and FTIR spectroscopy. ► Results demonstrated the intercalative binding between DNA and methotraxate.

Local detection of mechanically induced ATP release from bone cells with ATP microbiosensors   Original Research Article

Pages 27-33
Elena Hecht, Astrid Liedert, Anita Ignatius, Boris Mizaikoff, Christine Kranz


► Miniaturized ATP biosensors. ► Scanning electrochemical microscopy combined with a cell stretching device. ► Mechanically induced ATP release at MC3T3-E1 osteoblastic cells.

Fabrication of a new electrochemical sensor based on a new nano-molecularly imprinted polymer for highly selective and sensitive determination of tramadol in human urine samples   Original Research Article

Pages 34-40
Abbas Afkhami, Hamed Ghaedi, Tayyebeh Madrakian, Mazaher Ahmadi, Hedye Mahmood-Kashani


► A new nano-molecularly imprinted polymer bead was synthesized. ► The prepared nano-polymer bead was characterized by different methods. ► The bead was used to the fabrication of a chemically modified CPE. ► The electrode was used to the rapid and selective determination of tramadol.

Horseradish peroxidase-catalyzed synthesis of poly(thiophene-3-boronic acid) biocomposites for mono-/bi-enzyme immobilization and amperometric biosensing   Original Research Article

Pages 41-47
Yi Huang, Wen Wang, Zou Li, Xiaoli Qin, Lijuan Bu, Zhiyong Tang, Yingchun Fu, Ming Ma, Qingji Xie, Shouzhuo Yao, Jiming Hu


► Preparation of mono-/bi-enzyme biocomposites by enzymatic polymerization. ► Boronic acid polymer as a biocompatible matrix for enzyme immobilization. ► High-performance biosensing of H2O2and glucose.

A novel enzyme-free and label-free fluorescence aptasensor for amplified detection of adenosine   Original Research Article

Pages 52-56
Bo Fu, Jichao Cao, Wei Jiang, Lei Wang


► A novel fluorescence amplified aptasensor was developed. ► The aptasensor was based on target-catalyzed self-hairpin assembly. ► The aptasensor was enzyme-free and label-free, making it simple and inexpensive. ► The limit of detection of the aptasensor for adenosine was 6μM. ► This strategy provided a simple, rapid and high selective versatile platform.



► We develop an electrochemical aptasensor for IFN-γ detection. ► The detection method is based on graphene and nuclease cleavage amplification. ► Signal transduction and amplification of graphene on the MHA/SAM electrode. ► It is a label-free, highly sensitive strategy for the detection of IFN-γ. ► The assay has good response in complicated cell media.



► Constructed a BOx/SiO2@ZrONPs/CHIT/Au hybrid film. ► Fabricated an improved amperometric bilirubin biosensor based on this film. ► Biosensor had a detection limit of 0.1nM and linear range of 0.02–250μM. ► Employed for bilirubin determination in different serum samples.



► A selective electrochemical molecularly imprinted sensor for the detection of neomycin was developed. ► CS-SNP and GR-MWCNTs nanocomposites were used to improve the sensitivity of the sensor. ► MIPs were synthesized by electropolymerization using neomycin as the template, and pyrrole as the monomer. ► The sensor showed high selectivity, excellent stability and good reproducibility for the determination of neomycin. ► The developed sensor was successfully used for neomycin determination in real spiked samples.

Integration of gold nanoparticles in PDMS microfluidics for lab-on-a-chip plasmonic biosensing of growth hormones   Original Research Article

Pages 77-84
Hamid SadAbadi, Simona Badilescu, Muthukumaran Packirisamy, Rolf Wüthrich


► Anin-situmethod for integration of gold NP in a PDMS microfluidic chip is proposed. ► The kinetics is fully studied and the activation energy is estimated to be 30kJ/mol. ► The reaction in microchannel is found to be slower than at the macro-scale. ► The size uniformity of GNPs is found to be considerably improved in the micro-environment. ► A detection limit of 4ng/mL for bovine GH is obtained for the proposed biosensor.

Porous silicon optical microcavity biosensor on silicon-on-insulator wafer for sensitive DNA detection   Original Research Article

Pages 89-94
Hongyan Zhang, Zhenhong Jia, Xiaoyi Lv, Jun Zhou, Liangliang Chen, Rongxia Liu, Ji Ma


► A sensitive label-free PSM biosensor on SOI wafer was fabricated by electrochemical etching. ► By simulations and experiments, we optimized PSM biosensor with a high Q value and a high sensitivity. ► This biosensor was used for DNA detection and red shift shows a good linear relationship with DNA. ► This optical PSM on SOI could be a great potential for biochips based on integrated optical devices.

In situ amplification signaling-based autonomous aptameric machine for the sensitive fluorescence detection ofcocaine   Original Research Article

Pages 95-100
Su-Jin Xie, Hui Zhou, Dengyou Liu, Guo-Li Shen, Ruqin Yu, Zai-Sheng Wu


► In situ amplification signaling-based aptameric machine has been developed. ► Discovering operation behavior of scission/replication-based autonomous machine. ► The developed aptameric machine exhibits substantially improved assay characteristics.

Multiplex electrochemiluminescence immunoassay of two tumor markers using multicolor quantum dots as labels and graphene asconductingbridge   Original Research Article

Pages 101-107
Zhiyong Guo, Tingting Hao, Shuping Du, Beibei Chen, Zebo Wang, Xing Li, Sui Wang


► A multiplex ECL immunoassay of two tumor markers was proposed. ► Multicolor QDs were labeled to obtain distinguishable ECL signals. ► G was used as a conducting bridge, improving the ECL intensity about 30-folds. ► LOD for AFP and CEA was about 0.4fg/mL.

Nanogold-enhanced graphene nanosheets as multienzyme assembly for sensitive detection of low-abundanceproteins   Original Research Article

Pages 108-114
Huafeng Chen, Zhuangqiang Gao, Yuling Cui, Guonan Chen, Dianping Tang


► We introduce a new electrochemical immunoassay protocol for sensitive detection of low-abundance proteins. ► Using graphene-carried poly(o-phenylenediamine)/gold hybrid nanosheets as molecular tags. ► Design of redox-active nanolabels.

Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM)   Original Research Article

Pages 115-121
Fang Wei, Jieping Yang, David T.W. Wong


► EFIRM technique has been established for measuring biomolecules in exosomes. ► It uses a non-uniform electrical field to release the RNA/proteins from exosome. ► We confirmedin vivoH460-CD63-GFP exosomes transported to blood and saliva. ► It provides an on-site detection of distal tumor-shed biomarkers in exosome.

Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals   Original Research Article

Pages 138-145
Ting Wei, Chao Zhang, Xin Xu, Michelle Hanna, Xiaohua Zhang, Yan Wang, Heping Dai, Wei Xiao


► SelectedyEGFPas a reporter. ► Chose and compared theRNR3 andHUG1 promoters as sensors. ► Constructed a yeast septuple mutant with seven genes deleted to enhance the detection sensitivity. ► The resulting biosensors are much more sensitive than previously reported similar systems.

Simultaneous electrochemical determination of guanosine and adenosine with graphene–ZrO2 nanocomposite modified carbon ionic liquid electrode   Original Research Article

Pages 146-151
Wei Sun, Xiuzhen Wang, Xiaohuan Sun, Ying Deng, Jun Liu, Bingxin Lei, Zhenfan Sun


► Graphene and ZrO2nanoparticles based nanocomposite were used for the electrode modification. ► Adenosine and guanosine were simultaneous detected by the modified electrode. ► The proposed method was applied to the human urine samples with satisfactory results.

Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain   Original Research Article

Pages 152-159
Ricardo M. Santos, Marcelo S. Rodrigues, João Laranjinha, Rui M. Barbosa


► A microsensor for nitric oxide (NO) is designed based on a novel nanocomposite film. ► Cyclic voltammetry shows direct electron transfer from hemin. ► The electrocatalytic reduction of NO provides high sensitivity and selectivity. ► The microsensor is able to resolve NO and oxygen peaks by square wave voltammmetry. ► Measurement of exogenously applied NO in vivo in the rat brain is demonstrated.

Direct label free ultrasensitive impedimetric DNA biosensor using dendrimer functionalized GaN nanowires   Original Research Article

Pages 164-170
Prasana Sahoo, Sumathi Suresh, Sandip Dhara, Garima Saini, S. Rangarajan, A.K. Tyagi


► Nanowire based in-situ detection of DNA hybridization is demonstrated. ► An ultra low detection limit down toaMtarget DNA solution has been achieved. ► The selectivity of the sensor probe is shown by monitoring the sensor response from other non-specific target DNA. ► Change in the interfacial polarization resistance serves the basis of the present DNA biosensor.

A label-free microRNA biosensor based on DNAzyme-catalyzed and microRNA-guided formation of a thin insulating polymer film   Original Research Article

Pages 171-176
Wei Shen, Huimin Deng, Yuqian Ren, Zhiqiang Gao


► A PCR-free and label-free microRNA biosensor is developed. ► The accumulative nature of the deposition of a thin insulating film drastically improves the detectability of the biosensor. ► The amplifier—DNAzyme was found to be much more tolerant than natural enzymes in terms of reaction conditions. ► Attempts were made in detecting miRNAs in total RNA extracted from cancer cells.

Label-free electrogenerated chemiluminescence biosensing method for trace bleomycin detection based on a Ru(phen)32+–hairpin DNA composite film electrode   Original Research Article

Pages 177-182
Yan Li, Cancan Huang, Jianbin Zheng, Honglan Qi, Wei Cao, Yinmao Wei


► Label-free ECL DNA-based biosensing method for bleomycin was developed. ► The ECL biosensing method is based on Fe(II)·BLMs-mediated DNA strand scission. ► The biosensing method showed high sensitivity and selectivity.

An electrochemical biosensor for ascorbic acid based on carbon-supported PdNinanoparticles   Original Research Article

Pages 183-190
Xin Zhang, Yang Cao, Sha Yu, Fengchun Yang, Pinxian Xi


► Carbon-supported PdNi nanoparticles (PdNi/C) prepared. ► A novel synthetic route for PdNi/C preparation. ► The performance was better than Pd/C because of PdNi synergistic effects. ► PdNi/C was employed for ascorbic acid determination. ► High selectivity, good stability, wide linear range and high sensitivity.

One-step fabrication of sub-10-nm plasmonic nanogaps for reliable SERS sensing of microorganisms   Original Research Article

Pages 191-197
Jing Chen, Gaowu Qin, Jiansheng Wang, Jiangyu Yu, Bo Shen, Song Li, Yuping Ren, Liang Zuo, Wen Shen, Biswajit Das


► SERS substrates with high-density of sub-10-nm nanogaps were fabricated by high pressure sputtering in one-step. ► We demonstrate the reliability and reproducibility of this clean substrate which has no remaining chemicals. ► Excellent SERS spectra of four kinds of microorganisms were acquired. ► By introducing sub-10-nm nanogaps, the SERS substrate enhancement factor is improved by 103times. ► Piecewise-linear relationship exists between the SERS intensity and the rhodamine 6G concentration.

Label-free impedimetric immunosensors for psoriasin—Increased reproducibility and sensitivity using an automated dispensing system   Original Research Article

Pages 198-203
Timothy R.J. Holford, Joanne L. Holmes, Stuart D. Collyer, Frank Davis, Séamus P.J. Higson


► Developed and optimised a label-free immunosensor for psoriasin. ► Antibodies to psoriasin immobilised using avidin–biotin or electrostatic interactions. ► Both manual and automated (BioDot AD3200 micro-dispenser) deposition used. ► Affinity immobilised sensors display much higher sensitivity than electrostatic. ► BioDot produced sensors display higher sensitivity and lower sample-to-sample variation. Levels of psoriasin from 250–10,000pgml−1can be clearly determined.

Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase   Original Research Article

Pages 204-209
Zhenzhen Chen, Xiangling Ren, Xianwei Meng, Longfei Tan, Dong Chen, Fangqiong Tang


► Biosensor was composed of QDs and enzymes, without any complex processes. ► BChE detected by one step enzyme reaction for the first time. ► We studied the detection mechanisms of turn-on and turn-off detection system. ► We demonstrated the application in real serum samples. ► This work presents a feasible approach for detecting some kinds of substrate.

A nanoporous membrane-based impedimetric immunosensor for label-free detection of pathogenic bacteria in whole milk   Original Research Article

Pages 210-215
Cho-Kyung Joung, Han-Nah Kim, Min-Cheol Lim, Tae-Joon Jeon, Hae-Yeong Kim, Young-Rok Kim


► Nanoporous membrane-based impedimetric immunosensor for pathogen detection in milk. ► Sensitivity and selectivity greatly enhanced by hyaluronic acid interface. ► Detection limit of <102cfu/ml forE. coliO157 in whole milk.

A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein   Original Research Article

Pages 216-221
Ana Carolina M.S. Dias, Sérgio L.R. Gomes-Filho, Mízia M.S. Silva, Rosa F. Dutra


► A disposable immunosensor based on carbon nanotube-screen printed electrode was developed for NS1 protein of the dengue virus. ► Anti-NS1 antibodies were covalently bound to the CNT-tip surface by ethylenediamine film. ► Good limit of detection (12ngmL−1) was obtained and serum samples were analyzed.

Rapid, quantitative, reverse transcription PCR in a polymer microfluidicchip   Original Research Article

Pages 222-228
D. Curtis Saunders, Gregory L. Holst, Christopher R. Phaneuf, Nikita Pak, Matthew Marchese, Nicholas Sondej, Michael McKinnon, Craig R. Forest


A user-friendly instrument for microfluidic quantitative PCR and RT-PCR is reported. ► Plastic, disposable microchips were used to contain 1μl reaction volumes. ► Precision mechanical alignment and thermal stability allow repeatable open-loop control. ► Quantitative PCR and RT-PCR with viral DNA and GAPDH RNA were demonstrated. ► Electrophoretic end-point detection and melting curve analysis confirmed amplifications.

Selective virus detection in complex sample matrices with photonic crystal optical cavities   Original Research Article

Pages 229-234
Sudeshna Pal, Amrita R. Yadav, Mark A. Lifson, James E. Baker, Philippe M. Fauchet, Benjamin L. Miller


► 2-D Photonic Crystals are optical sensors with an exceptionally small sensing volume. ► We conducted FDTD calculations that suggest single virus detection is possible. ► Detection of human papillomavirus virus-like particles (VLPs) was demonstrated. ► High selectivity was obtained in buffer and in serum.



► Proposed strategy to fabricate the graphene–gold nanocomposite and sensor is green and controllable. ► Graphene–gold nanocomposite remarkably enhance the electron transfer rate. ► The antibody was covalently immobilized on the electrode using functional conducing polymer. ► The ionic liquid improves biocompatibility of the microenvironment for the antibody. ► The sensor for microcystin-LR offers the best sensitivity and stability up to now.

Integrated biochip for label-free and real-time detection of DNA amplification by contactless impedance measurements based on interdigitated electrodes   Original Research Article

Pages 241-247
Xinxin Fang, Qinghui Jin, Fengxiang Jing, Huanqian Zhang, Feng Zhang, Hongju Mao, Baojian Xu, Jianlong Zhao


► Integrated biochip for impedance detection of DNA amplification in real-time is reported. ► The chip includes temperature controlling electrodes and interdigitated EC electrodes. ► The biochip exhibits rapid detection efficiency and high sensitivity for SDA. ► The biochip is developed first to monitor RCA in real-time.

  Short Communications



► A simple PNK activity assay using dsDNA-templated CuNPs as a fluorescent indicator was presented. ► Any other additional modifications of DNA substrate or complex design were not required for this method. ► PNK activity was sensitively and selectively detected. ► In complex biological samples, this method exhibited a good assay performance.


Graphical abstract


► A universal PEC sensing platform was fabricated based on a ternary composition of PPIX–WO3–rGO. ► The multiple electron transfer system of PPIX–WO3–rGO obtained enhanced photocurrent. ► A highly sensitive and selective biosensor for PEC detection of cysteine developed. ► The proposed PEC sensor opened up a new avenue to the application of WO3nanostructures.

Sensitive amperometric biosensor for phenolic compounds based on graphene–silk peptide/tyrosinase composite nanointerface   

Pages 85-88
Ying Qu, Ming Ma, Zhengguo Wang, Guoqing Zhan, Buhai Li, Xian Wang, Huaifang Fang, Huijuan Zhang, Chunya Li


►Silk peptide functionalized graphene nanosheets were synthesized and fully characterized;►Graphene–silk peptide–tyrosinase/glassy carbon electrodes (Gr–SP–Tyr/GCE) were fabricated;►Electrochemical behaviors of phenolic compounds at Gr–SP–Tyr/GCE were investigated;►Sensitive amperometric method for phenolic compounds was developed;►Practical application of the biosensor was demonstrated.

Development of a novel electrochemical sensor using pheochromocytoma cells and its assessment of acrylamide cytotoxicity   

Pages 122-126
Xiulan Sun, Jian Ji, Donglei Jiang, Xiaowei Li, Yinzhi Zhang, Zaijun Li, Yongning Wu


► A novel living cell-based electrochemical sensor was fabricated. ► Graphene–gold nanocomposite as signal amplification was used for living cell immobilization. ► The electrochemical sensor was successfully used for monitoring toxic effects of acrylamide on PC-12 cells.

A one-pot ‘green’ synthesis of Pd-decorated PEDOT nanospheres for nonenzymatic hydrogen peroxide sensing   

Pages 127-131
Fengxing Jiang, Ruirui Yue, Yukou Du, Jingkun Xu, Ping Yang

Graphical abstract


► One-pot facile synthesis of Pd-decorated homogeneous PEDOT nanospheres. ► No any surfactant and template to be used. ► 3D porous nanostructure for Pd/PEDOT nanospheres. ► Well-dispersed Pd nanoparticles on PEDOT nanospheres. ► High sensitivity and low detection limit for Pd/PEDOT as H2O2sensor.

CdTe nanobioprobe based optoelectrochemical immunodetection of diabetic marker HbA1c   

Pages 132-135
Adity Chopra, Satish Tuteja, Naresh Sachdeva, K.K. Bhasin, Vijayender Bhalla, C. Raman Suri


► Highly luminescent aqueous CdTe quantum dots synthesized. ► Quantum dots conjugated with specific antibody used as nanobioprobe. ► Dual fluorescence cum electrochemical format for HbA1c detection. ► Correlation coefficient of 96% for electrochemical detection with HPLC. ► Highly reproducible and promising HbA1c detection platform.

Direct energy conversion from xylose using xylose dehydrogenase surface displayed bacteria based enzymatic biofuel cell   

Pages 160-163
Lin Xia, Bo Liang, Liang Li, Xiangjiang Tang, Ilaria Palchetti, Marco Mascini, Aihua Liu


► First report on the direct energy conversion from xylose using xylose dehydrogenase displayed bacteria based biofuel cell. ► Maximum power density of the cell up to 63μWcm−2. ► The open-circuit potential of the cell up to 0.58V.

  Book Review


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