World Congress on Biosensors 2014

World Congress on Biosensors 2014
Biosensors 2014

Friday, 5 April 2013

Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:
Selected papers from the latest issue:



► A sensitive method for analysis of bioactives of ginseng is proposed. ► Method combined equilibrium dialysis and UPLC-MS/MS. ► The study of the interactions between ginseng and liposome is firstly reported. ► The method offers a theoretical direction of potential bioactivities in ginseng.

Optimization of on-line solid phase extraction and HPLC conditions using response surface methodology for determination of WM-5 in mouse plasma and its application to pharmacokinetic study   Original Research Article

Pages 8-15
Lei Liu, Ya-Bin Wen, Kang-Ning Liu, Liang Sun, Meng Wu, Gui-Fang Han, Ya-Xin Lu, Qing-Ming Wang, Zheng Yin


► Fully automated on-line SPE–HPLC utilized for PK evaluation of WM-5. ► Response surface methodology used for optimization of on-line SPE parameters. ► On-line SPE removed the time-consuming, tedious and costly manual process. ► The method is simple, fast, specific and sensitive.



► Deglycosylation systematically improved proteomic analyses containing glycoproteins. ► AspN and trypsin provided complementary analysis with deglycosylation. ► Improvement in identification correlated with the density of glycosylation sites. ► Cis3p, important for cell wall integrity, was upregulated inΔalg3yeast.

Determination of methylphenidate in plasma and saliva by liquid chromatography/tandem mass spectrometry   Original Research Article

Pages 22-28
A. Seçilir, L. Schrier, Y.A. Bijleveld, J.H. Toersche, S. Jorjani, J. Burggraaf, J. van Gerven, R.A.A. Mathôt


► A LC–MS/MS method was validated for the determination of methylphenidate (MPH) in plasma and saliva. ► The method allows fast, accurate and precise quantification of MPH in both plasma and saliva. ► The use of deuterated MPH as an internal standard is essential, due to matrix effects of plasma. ► MPH is degraded in plasma and saliva; clinical samples should be stored at −20°C directly after sampling.

Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC–DAD: A cocktail approach   Original Research Article

Pages 29-36
Wensi Lin, Jianmei Zhang, Xiaomei Ling, Ning Yu, Jing Li, Haisong Yang, Runtao Li, Jingrong Cui


► On-line solid-phase extraction HPLC–DAD method was applied to determine five specific probe substratesin vivo. ► The method was applied to evaluate the effects of TM208 on rat five CYP450 isoforms. ► TM208 had the potential to induce the metabolic activities of CYP2E1 and CYP3A4 in rats. ► TM208 might not significantly affect CYP1A2, CYP2D6 and CYP2C19-mediated metabolism in rats.

Development of a subcritical fluid extraction and GC–MS validation method for polychlorinated biphenyls (PCBs) in marine samples   Original Research Article

Pages 37-42
Kai Jia, Xiaomei Feng, Kun Liu, Yuqian Han, Yong Xue, Changhu Xue


► A method for determination of PCBs in marine products was developed. ► 1,1,1,2-Tetrafluoroethane (R134a) is used as subcritical fluid. ► Several factors were employed to optimize the conditions of the extraction. ► High extraction efficiency could be obtained at low pressure and temperature. ► The proposed method was successfully applied to the real samples.



► UF-LC–MS method for screening XOD inhibitors was developed. ► Method is suited for rapid screening and characterization of XOD inhibitors from TCM. ► UF-LC–MS is a rapid, sensitivity and high throughput tool to screen XOD inhibitors. ► Eleven XOD inhibitors were screened from theRadix Salvia Miltiorrhizaeextract. ► The relationship between the structure and activity of inhibitors was estimated.

Analysis of iridoid glucosides from Paederia scandens using HPLC–ESI-MS/MS   Original Research Article

Pages 54-64
Zhi-Jun Wu, Jian-Hua Wang, Dong-Mei Fang, Guo-Lin Zhang


► A total of 24 iridoid glucosides (14 new ones) were characterized using HPLC–ESI-MS. ► The difference mainly focuses on the side chain of iridoid moiety for simple ones. ► The connection order of iridoid, sugar and phenolic acid moieties can be confirmed. ► The acylation of 6-OH of sugar by phenolic acid can be determinated. ► Connection order between asperuloside and paederoside moieties can be confirmed.

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots   Original Research Article

Pages 65-73
N. Mohamed Shah, A.F. Hawwa, J.S. Millership, P.S. Collier, J.C. McElnay


► We report a simple method for the analysis of four antiepileptic drugs in DBS samples. ► The method was applied to DBS samples collected from children with epilepsy. ► Such technique has potential in assessing adherence to AEDs using home sampling.

Evaluating the reproducibility of quantifying modified nucleosides from ribonucleic acids by LC–UV–MS   Original Research Article

Pages 74-82
Susan P. Russell, Patrick A. Limbach


► Evaluation of LC/UV/MS conditions for quantitative analysis of modified nucleosides. ► Minor (low abundance) RNA modifications reproducibly analyzed. ► Biological variability in modified nucleosides<10% RSD. ► tRNA modifications easier to quantify than rRNA modifications. ► Column temperature can be adjusted to improve separation window.



► Polar endogenous compounds in plasma identified to interfere in HILIC–ESI-MS. ► Both enhancement and suppression of the analyte signal was observed. ► Choosing columns with different selectivity alters the regions of suppression/enhancement.



► First report on n-octanol/water partition of treosulfan and its epoxy-transformers. ► Results of logPcalculated by ALOGPs correlated best with the experimental values. ► Negative correlation between logPof the analytes and their retention in RP-HPLC. ► Treosulfan and its epoxides do not constitute a ‘congeneric’ series of compounds. ► Hydrophilic nature of treosulfan corresponds to its pharmacokinetic parameters.

Native chromatographic sample preparation of serum, plasma and cerebrospinal fluid does not comprise a risk for proteolytic biomarker loss   Original Research Article

Pages 102-109
Jelena Pesek, Thomas Krüger, Nadine Krieg, Michael Schiel, Johannes Norgauer, Julian Großkreutz, Heidrun Rhode


► We recently developed a native fractionation method for serum, plasma and CSF. ► Proteolysis was tested during usual and prolonged sample handling and preparation. ► No evidence of increased peptide fractions was found after variable incubation time. ► Virtually no proteolytic activity was observed after moderate trypsin addition. ► There is practically no risk of biomarker loss by intrinsic proteinases with our method.

Quantitative determination of nebivolol from human plasma using liquid chromatography–tandem mass spectrometry   Original Research Article

Pages 110-119
Jatin Nandania, S.J. Rajput, Pritesh Contractor, Pragnesh Vasava, Bhavik Solanki, Mohsin Vohra


► Liquid–liquid extraction method was used to extract nebivolol from human plasma. ► Liquid chromatography with tandem mass spectrometry was used to analyze and quantify nebivolol. ► Method was validated in terms of accuracy, precision, selectivity, absolute recovery and stability. ► This method is free from ion suppression, ion enhancement and any type of abnormal ionization. ► Developed bioanalytical method is a highly sensitive as lower limit of quantification was proved 30pg/mL.

Determination of 3β-hydroxy-Δ5-bile acids and related compounds in biological fluids of patients with cholestasis by liquid chromatography–tandem mass spectrometry   Original Research Article

Pages 120-127
Tsuyoshi Murai, Kana Oda, Terutake Toyo, Hiroshi Nittono, Hajime Takei, Akina Muto, Akihiko Kimura, Takao Kurosawa


► We developed an LC–MS/MS method for determination of 3β-hydroxy-Δ5-bile acids. ► We analyzed urine and serum from patients with 3β-dehydrogenase deficiency. ► These bile acids were the major bile acids in urine and serum from those patients. ► This method is suitable for determination of these bile acids in clinical analysis.

Detection of main urinary metabolites of β2-agonists clenbuterol, salbutamol and terbutaline by liquid chromatography high resolution mass spectrometry   Original Research Article

Pages 128-135
Juan C. Domínguez-Romero, Juan F. García-Reyes, Rubén Martínez-Romero, Esther Martínez-Lara, María L. Del Moral-Leal, Antonio Molina-Díaz


► Urinary metabolism study of clenbuterol, salbutanol and terbutaline in rat. ► Methodology based on solid-phase extraction followed by LC-Electrospray-TOFMS. ► Use of diagnostic fragments ions and accurate mass shifts from biotransformations. ► Up to eleven metabolites detected, four of them non previously reported in urine. ► Three clenbuterol metabolites were detected over a longer period than parent drug.



► A new MI-MSPD-HPLC method is developed for rapid screening of clenbuterol in chicken. ► Dummy molecularly imprinted microspheres are synthesized by suspension polymerization. ► The extraction selectivity is significantly improved. ► The effect of template leaking on quantitative analysis is avoided.

  Short Communications

Determination of Raddeanin A in rat plasma by liquid chromatography–tandem mass spectrometry: Application to a pharmacokinetic study   

Pages 43-47
Xin Luan, Ying-Yun Guan, Chao Wang, Mei Zhao, Qin Lu, Ya-Bin Tang, Ya-Rong Liu, De-Hong Yu, Xiao-Lin Wang, Hong Qi, Chao Fang, Hong-Zhuan Chen


► A sensitive HPLC–MS/MS method forin vivoquantitative assay of Raddeanin A was developed. ► The method was characteristic of short running time and simple preparation process. ► The pharmacokinetics of Raddeanin A in rat after intravenous and intraperitoneal injection was evaluated.

Use of T-2 toxin-immobilized amine-activated beads as an efficient affinity purification matrix for the isolation of specific IgY   

Pages 98-101
Soujanya Ratna Edupuganti, Om Prakash Edupuganti, Richard O’Kennedy, Eric Defrancq, Stéphanie Boullanger


► Immobilization of T-2 toxin on amine-activated sepharose beads. ► Utilization of T-2 toxin-immobilized beads for increasing specificity of IgY with reduction in cross reactivity. ► Development of SPR-based inhibition assay and its validation.

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