Just Published: Journal of Chromatography B

A new issue of this journal has just been published. To see abstracts of the papers it contains (with links through to the full papers) click here:

Journal of Chromatography B
http://rss.sciencedirect.com/publication/science/7220

Selected papers from the latest issue:

2.

Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry   Original Research Article Pages 1-7 Guangyue Hou, Jun Niu, Fengrui Song, Zhiqiang Liu, Shuying Liu Highlights ► A sensitive method for analysis of bioactives of ginseng is proposed. ► Method combined equilibrium dialysis and UPLC-MS/MS. ► The study of the interactions between ginseng and liposome is firstly reported. ► The method offers a theoretical direction of potential bioactivities in ginseng.

3.

Optimization of on-line solid phase extraction and HPLC conditions using response surface methodology for determination of WM-5 in mouse plasma and its application to pharmacokinetic study   Original Research Article Pages 8-15 Lei Liu, Ya-Bin Wen, Kang-Ning Liu, Liang Sun, Meng Wu, Gui-Fang Han, Ya-Xin Lu, Qing-Ming Wang, Zheng Yin Highlights ► Fully automated on-line SPE–HPLC utilized for PK evaluation of WM-5. ► Response surface methodology used for optimization of on-line SPE parameters. ► On-line SPE removed the time-consuming, tedious and costly manual process. ► The method is simple, fast, specific and sensitive.

4.

Deglycosylation systematically improves N-glycoprotein identification in liquid chromatography–tandem mass spectrometry proteomics for analysis of cell wall stress responses in Saccharomyces cerevisiae lacking Alg3p   Original Research Article Pages 16-21 Ulla-Maja Bailey, Benjamin L. Schulz Highlights ► Deglycosylation systematically improved proteomic analyses containing glycoproteins. ► AspN and trypsin provided complementary analysis with deglycosylation. ► Improvement in identification correlated with the density of glycosylation sites. ► Cis3p, important for cell wall integrity, was upregulated inΔalg3yeast.

5.

Determination of methylphenidate in plasma and saliva by liquid chromatography/tandem mass spectrometry   Original Research Article Pages 22-28 A. Seçilir, L. Schrier, Y.A. Bijleveld, J.H. Toersche, S. Jorjani, J. Burggraaf, J. van Gerven, R.A.A. Mathôt Highlights ► A LC–MS/MS method was validated for the determination of methylphenidate (MPH) in plasma and saliva. ► The method allows fast, accurate and precise quantification of MPH in both plasma and saliva. ► The use of deuterated MPH as an internal standard is essential, due to matrix effects of plasma. ► MPH is degraded in plasma and saliva; clinical samples should be stored at −20°C directly after sampling.

6.

Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC–DAD: A cocktail approach   Original Research Article Pages 29-36 Wensi Lin, Jianmei Zhang, Xiaomei Ling, Ning Yu, Jing Li, Haisong Yang, Runtao Li, Jingrong Cui Highlights ► On-line solid-phase extraction HPLC–DAD method was applied to determine five specific probe substratesin vivo. ► The method was applied to evaluate the effects of TM208 on rat five CYP450 isoforms. ► TM208 had the potential to induce the metabolic activities of CYP2E1 and CYP3A4 in rats. ► TM208 might not significantly affect CYP1A2, CYP2D6 and CYP2C19-mediated metabolism in rats.

7.

Development of a subcritical fluid extraction and GC–MS validation method for polychlorinated biphenyls (PCBs) in marine samples   Original Research Article Pages 37-42 Kai Jia, Xiaomei Feng, Kun Liu, Yuqian Han, Yong Xue, Changhu Xue Highlights ► A method for determination of PCBs in marine products was developed. ► 1,1,1,2-Tetrafluoroethane (R134a) is used as subcritical fluid. ► Several factors were employed to optimize the conditions of the extraction. ► High extraction efficiency could be obtained at low pressure and temperature. ► The proposed method was successfully applied to the real samples.

8.

Screening and determination of potential xanthine oxidase inhibitors from Radix Salviae Miltiorrhizae using ultrafiltration liquid chromatography–mass spectrometry   Original Research Article Pages 48-53 Yang Liu, Shu Liu, Zhiqiang Liu Highlights ► UF-LC–MS method for screening XOD inhibitors was developed. ► Method is suited for rapid screening and characterization of XOD inhibitors from TCM. ► UF-LC–MS is a rapid, sensitivity and high throughput tool to screen XOD inhibitors. ► Eleven XOD inhibitors were screened from theRadix Salvia Miltiorrhizaeextract. ► The relationship between the structure and activity of inhibitors was estimated.

9.

Analysis of iridoid glucosides from Paederia scandens using HPLC–ESI-MS/MS   Original Research Article Pages 54-64 Zhi-Jun Wu, Jian-Hua Wang, Dong-Mei Fang, Guo-Lin Zhang Highlights ► A total of 24 iridoid glucosides (14 new ones) were characterized using HPLC–ESI-MS. ► The difference mainly focuses on the side chain of iridoid moiety for simple ones. ► The connection order of iridoid, sugar and phenolic acid moieties can be confirmed. ► The acylation of 6-OH of sugar by phenolic acid can be determinated. ► Connection order between asperuloside and paederoside moieties can be confirmed.

10.

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots   Original Research Article Pages 65-73 N. Mohamed Shah, A.F. Hawwa, J.S. Millership, P.S. Collier, J.C. McElnay Highlights ► We report a simple method for the analysis of four antiepileptic drugs in DBS samples. ► The method was applied to DBS samples collected from children with epilepsy. ► Such technique has potential in assessing adherence to AEDs using home sampling.

11.

Evaluating the reproducibility of quantifying modified nucleosides from ribonucleic acids by LC–UV–MS   Original Research Article Pages 74-82 Susan P. Russell, Patrick A. Limbach Highlights ► Evaluation of LC/UV/MS conditions for quantitative analysis of modified nucleosides. ► Minor (low abundance) RNA modifications reproducibly analyzed. ► Biological variability in modified nucleosides<10% RSD. ► tRNA modifications easier to quantify than rRNA modifications. ► Column temperature can be adjusted to improve separation window.

12.

Tracing and separating plasma components causing matrix effects in hydrophilic interaction chromatography–electrospray ionization mass spectrometry   Original Research Article Pages 83-91 Anja Ekdahl, Maria C. Johansson, Martin Ahnoff Highlights ► Polar endogenous compounds in plasma identified to interfere in HILIC–ESI-MS. ► Both enhancement and suppression of the analyte signal was observed. ► Choosing columns with different selectivity alters the regions of suppression/enhancement.

13.

Determination of partition coefficients n-octanol/water for treosulfan and its epoxy-transformers: An example of a negative correlation between lipophilicity of unionized compounds and their retention in reversed-phase chromatography   Original Research Article Pages 92-97 Franciszek K. Główka, Michał Romański, Anna Siemiątkowska Highlights ► First report on n-octanol/water partition of treosulfan and its epoxy-transformers. ► Results of logPcalculated by ALOGPs correlated best with the experimental values. ► Negative correlation between logPof the analytes and their retention in RP-HPLC. ► Treosulfan and its epoxides do not constitute a ‘congeneric’ series of compounds. ► Hydrophilic nature of treosulfan corresponds to its pharmacokinetic parameters.

14.

Native chromatographic sample preparation of serum, plasma and cerebrospinal fluid does not comprise a risk for proteolytic biomarker loss   Original Research Article Pages 102-109 Jelena Pesek, Thomas Krüger, Nadine Krieg, Michael Schiel, Johannes Norgauer, Julian Großkreutz, Heidrun Rhode Highlights ► We recently developed a native fractionation method for serum, plasma and CSF. ► Proteolysis was tested during usual and prolonged sample handling and preparation. ► No evidence of increased peptide fractions was found after variable incubation time. ► Virtually no proteolytic activity was observed after moderate trypsin addition. ► There is practically no risk of biomarker loss by intrinsic proteinases with our method.

15.

Quantitative determination of nebivolol from human plasma using liquid chromatography–tandem mass spectrometry   Original Research Article Pages 110-119 Jatin Nandania, S.J. Rajput, Pritesh Contractor, Pragnesh Vasava, Bhavik Solanki, Mohsin Vohra Highlights ► Liquid–liquid extraction method was used to extract nebivolol from human plasma. ► Liquid chromatography with tandem mass spectrometry was used to analyze and quantify nebivolol. ► Method was validated in terms of accuracy, precision, selectivity, absolute recovery and stability. ► This method is free from ion suppression, ion enhancement and any type of abnormal ionization. ► Developed bioanalytical method is a highly sensitive as lower limit of quantification was proved 30pg/mL.

16.

Determination of 3β-hydroxy-Δ5-bile acids and related compounds in biological fluids of patients with cholestasis by liquid chromatography–tandem mass spectrometry   Original Research Article Pages 120-127 Tsuyoshi Murai, Kana Oda, Terutake Toyo, Hiroshi Nittono, Hajime Takei, Akina Muto, Akihiko Kimura, Takao Kurosawa Highlights ► We developed an LC–MS/MS method for determination of 3β-hydroxy-Δ5-bile acids. ► We analyzed urine and serum from patients with 3β-dehydrogenase deficiency. ► These bile acids were the major bile acids in urine and serum from those patients. ► This method is suitable for determination of these bile acids in clinical analysis.

17.

Detection of main urinary metabolites of β2-agonists clenbuterol, salbutamol and terbutaline by liquid chromatography high resolution mass spectrometry   Original Research Article Pages 128-135 Juan C. Domínguez-Romero, Juan F. García-Reyes, Rubén Martínez-Romero, Esther Martínez-Lara, María L. Del Moral-Leal, Antonio Molina-Díaz Highlights ► Urinary metabolism study of clenbuterol, salbutanol and terbutaline in rat. ► Methodology based on solid-phase extraction followed by LC-Electrospray-TOFMS. ► Use of diagnostic fragments ions and accurate mass shifts from biotransformations. ► Up to eleven metabolites detected, four of them non previously reported in urine. ► Three clenbuterol metabolites were detected over a longer period than parent drug.

18.

Rapid screening of clenbuterol hydrochloride in chicken samples by molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography   Original Research Article Pages 136-140 Fengxia Qiao, Jingjing Du Highlights ► A new MI-MSPD-HPLC method is developed for rapid screening of clenbuterol in chicken. ► Dummy molecularly imprinted microspheres are synthesized by suspension polymerization. ► The extraction selectivity is significantly improved. ► The effect of template leaking on quantitative analysis is avoided.

Short Communications

19.

Determination of Raddeanin A in rat plasma by liquid chromatography–tandem mass spectrometry: Application to a pharmacokinetic study    Pages 43-47 Xin Luan, Ying-Yun Guan, Chao Wang, Mei Zhao, Qin Lu, Ya-Bin Tang, Ya-Rong Liu, De-Hong Yu, Xiao-Lin Wang, Hong Qi, Chao Fang, Hong-Zhuan Chen Highlights ► A sensitive HPLC–MS/MS method forin vivoquantitative assay of Raddeanin A was developed. ► The method was characteristic of short running time and simple preparation process. ► The pharmacokinetics of Raddeanin A in rat after intravenous and intraperitoneal injection was evaluated.

20.

Use of T-2 toxin-immobilized amine-activated beads as an efficient affinity purification matrix for the isolation of specific IgY    Pages 98-101 Soujanya Ratna Edupuganti, Om Prakash Edupuganti, Richard O’Kennedy, Eric Defrancq, Stéphanie Boullanger Highlights ► Immobilization of T-2 toxin on amine-activated sepharose beads. ► Utilization of T-2 toxin-immobilized beads for increasing specificity of IgY with reduction in cross reactivity. ► Development of SPR-based inhibition assay and its validation.