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papers from the latest issue:
Fe3O4 magnetic nanoparticles/reduced graphene oxide nanosheets as a novel electrochemical and bioeletrochemical sensing platform
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Hazhir Teymourian , Abdollah Salimi , Somayeh Khezrian
We have developed Fe3O4 magnetic nanoparticles/reduced graphene oxide nanosheets modified glassy carbon (Fe3O4/r-GO/GC) electrode as a novel system for the preparation of electrochemical sensing platform. Decorating Fe3O4 nanoparticles on graphene sheets was performed via a facile one-step chemical reaction strategy, where the reduction of GO and the in-situ generation of Fe3O4 nanoparticles occurred simultaneously. Characterization of as-made nanocomposite using X-ray diffraction (XRD), transmission electron microscopy (TEM) and alternative gradient force magnetometry (AGFM) clearly demonstrate the successful attachment of monodisperse Fe3O4 nanoparticles to graphene sheets. Electrochemical studies revealed that the Fe3O4/r-GO/GC electrode possess excellent electrocatalytic activities toward the low potential oxidation of NADH (0.05V vs. Ag/AgCl) as well as the catalytic reduction of O2 and H2O2 at reduced overpotentials. Via immobilization of lactate dehydrogenase (LDH) as a model dehydrogenase enzyme onto the Fe3O4/r-GO/GC electrode surface, the ability of modified electrode for biosensing lactate was demonstrated. In addition, using differential pulse voltammetry (DPV) to investigate the electrochemical oxidation behavior of ascorbic acid (AA), dopamine (DA) and uric acid (UA) at Fe3O4/r-GO/GC electrode, the high electrocatalytic activity of the modified electrode toward simultaneous detection of these compounds was indicated. Finally, based on the strong electrocatalytic action of Fe3O4/r-GO/GC electrode toward both oxidation and reduction of nitrite, a sensitive amperometric sensor for nitrite determination was proposed. The Fe3O4/r-GO hybrid presented here showing favorable electrochemical features may hold great promise to the development of electrochemical sensors, molecular bioelectronic devices, biosensors and biofuel cells.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Hazhir Teymourian , Abdollah Salimi , Somayeh Khezrian
We have developed Fe3O4 magnetic nanoparticles/reduced graphene oxide nanosheets modified glassy carbon (Fe3O4/r-GO/GC) electrode as a novel system for the preparation of electrochemical sensing platform. Decorating Fe3O4 nanoparticles on graphene sheets was performed via a facile one-step chemical reaction strategy, where the reduction of GO and the in-situ generation of Fe3O4 nanoparticles occurred simultaneously. Characterization of as-made nanocomposite using X-ray diffraction (XRD), transmission electron microscopy (TEM) and alternative gradient force magnetometry (AGFM) clearly demonstrate the successful attachment of monodisperse Fe3O4 nanoparticles to graphene sheets. Electrochemical studies revealed that the Fe3O4/r-GO/GC electrode possess excellent electrocatalytic activities toward the low potential oxidation of NADH (0.05V vs. Ag/AgCl) as well as the catalytic reduction of O2 and H2O2 at reduced overpotentials. Via immobilization of lactate dehydrogenase (LDH) as a model dehydrogenase enzyme onto the Fe3O4/r-GO/GC electrode surface, the ability of modified electrode for biosensing lactate was demonstrated. In addition, using differential pulse voltammetry (DPV) to investigate the electrochemical oxidation behavior of ascorbic acid (AA), dopamine (DA) and uric acid (UA) at Fe3O4/r-GO/GC electrode, the high electrocatalytic activity of the modified electrode toward simultaneous detection of these compounds was indicated. Finally, based on the strong electrocatalytic action of Fe3O4/r-GO/GC electrode toward both oxidation and reduction of nitrite, a sensitive amperometric sensor for nitrite determination was proposed. The Fe3O4/r-GO hybrid presented here showing favorable electrochemical features may hold great promise to the development of electrochemical sensors, molecular bioelectronic devices, biosensors and biofuel cells.
A cardiomyocyte-based biosensor for antiarrhythmic drug evaluation by simultaneously monitoring cell growth and beating
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Tianxing Wang , Ning Hu , Jiayue Cao , Jieying Wu , Kaiqi Su , Ping Wang
Drug-induced cardiotoxicity greatly endangers the human health and results in resource waste. Also, it is a leading attribution to drug withdrawal and late-stage attrition in pharmaceutical industry. In the study, a dual function cardiomyocyte-based biosensor was introduced for rapid drug evaluation with xCELLigence RTCA Cardio system. The cardiomyocyte-based biosensor can monitor the cardiomyocyte growth and beating status simultaneously under the drug effects. Two typical cardiovascular drug, verapamil and flecainide were selected as treatment agents to test the performance of this biosensor. The experiment results showed that the performance of cardiomyocyte-based biosensor verified the basic drug effects by beating status and also tested the drug cytotoxicity by the cell index curves of cardiomyocyte growth. Based on the advanced sensor detection technology and cell culture technology, this cardiomyocyte-based biosensor will be a utility platform for the drug preclinical assessment.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Tianxing Wang , Ning Hu , Jiayue Cao , Jieying Wu , Kaiqi Su , Ping Wang
Drug-induced cardiotoxicity greatly endangers the human health and results in resource waste. Also, it is a leading attribution to drug withdrawal and late-stage attrition in pharmaceutical industry. In the study, a dual function cardiomyocyte-based biosensor was introduced for rapid drug evaluation with xCELLigence RTCA Cardio system. The cardiomyocyte-based biosensor can monitor the cardiomyocyte growth and beating status simultaneously under the drug effects. Two typical cardiovascular drug, verapamil and flecainide were selected as treatment agents to test the performance of this biosensor. The experiment results showed that the performance of cardiomyocyte-based biosensor verified the basic drug effects by beating status and also tested the drug cytotoxicity by the cell index curves of cardiomyocyte growth. Based on the advanced sensor detection technology and cell culture technology, this cardiomyocyte-based biosensor will be a utility platform for the drug preclinical assessment.
A silver–palladium alloy nanoparticle-based electrochemical biosensor for simultaneous detection of ractopamine, clenbuterol and salbutamol
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Huan Wang , Yong Zhang , He Li , Bin Du , Hongmin Ma , Dan Wu , Qin Wei
A multiplexed electrochemical biosensor has been developed for fast and sensitive detection of ractopamine (RAC), salbutamol (SAL) and clenbuterol (CLB) based on reduced graphene oxide (rGO) and silver–palladium alloy nanoparticles (AgPd NPs). In this paper, rGO with high conductivity was used as an electrode material to immobilize artificial antigens and amplify electrochemical signal. AgPd NPs are used to label antibodies and generate a strong electrochemical signal in phosphate buffered saline (PBS) without any other substrates. Screen-printed carbon electrode (SPCE) and competition strategy were adopted to achieve simultaneous detection of RAC, SAL and CLB without cross-talk between adjacent electrodes. This method can simultaneously detect RAC, SAL and CLB ranging from 0.01 to 100ngmL−1 with detection limits of 1.52pgmL−1, 1.44pgmL−1 and 1.38pgmL−1, respectively. Satisfactory results are achieved in pork sample analysis. The designed strategy provides a promising potential in determination of other biological samples.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Huan Wang , Yong Zhang , He Li , Bin Du , Hongmin Ma , Dan Wu , Qin Wei
A multiplexed electrochemical biosensor has been developed for fast and sensitive detection of ractopamine (RAC), salbutamol (SAL) and clenbuterol (CLB) based on reduced graphene oxide (rGO) and silver–palladium alloy nanoparticles (AgPd NPs). In this paper, rGO with high conductivity was used as an electrode material to immobilize artificial antigens and amplify electrochemical signal. AgPd NPs are used to label antibodies and generate a strong electrochemical signal in phosphate buffered saline (PBS) without any other substrates. Screen-printed carbon electrode (SPCE) and competition strategy were adopted to achieve simultaneous detection of RAC, SAL and CLB without cross-talk between adjacent electrodes. This method can simultaneously detect RAC, SAL and CLB ranging from 0.01 to 100ngmL−1 with detection limits of 1.52pgmL−1, 1.44pgmL−1 and 1.38pgmL−1, respectively. Satisfactory results are achieved in pork sample analysis. The designed strategy provides a promising potential in determination of other biological samples.
Acetylcholinesterase biosensor based on SnO2 nanoparticles–carboxylic graphene–nafion modified electrode for detection of pesticides
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Long Yang , Qing Zhou , Guangcan Wang , Yun Yang
A sensitive amperometric acetylcholinesterase (AChE) biosensor, based on SnO2 nanoparticles (SnO2 NPs), carboxylic graphene (CGR) and nafion (NF) modified glassy carbon electrode (GCE) for the detection of methyl parathion and carbofuran has been developed. The nanocomposites of SnO2 NPs and CGR was synthesized and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Chitosan (CS) was used to immobilize AChE on SnO2 NPs–CGR–NF/GCE and to improve electronic transmission between AChE and SnO2 NPs–CGR–NF/GCE. NF was used as the protective membrane for the AChE biosensor. The SnO2 NPs–CGR–NF nanocomposites with excellent conductivity, catalysis and biocompatibility offered an extremely hydrophilic surface for AChE adhesion. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl with an apparent Michaelis–Menten constant value of 131μM. The biosensor detected methyl parathion in the linear range from 10−13 to 10−10 M and from 10−10 to 10−8 M. The biosensor detected carbofuran in the linear range from 10−12 to 10−10 M and from 10−10 to 10−8 M. The detection limits of methyl parathion and carbofuran were 5×10−14 M and 5×10−13 M, respectively. The biosensor exhibited low applied potential, high sensitivity and acceptable stability, thus providing a promising tool for analysis of pesticides.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Long Yang , Qing Zhou , Guangcan Wang , Yun Yang
A sensitive amperometric acetylcholinesterase (AChE) biosensor, based on SnO2 nanoparticles (SnO2 NPs), carboxylic graphene (CGR) and nafion (NF) modified glassy carbon electrode (GCE) for the detection of methyl parathion and carbofuran has been developed. The nanocomposites of SnO2 NPs and CGR was synthesized and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Chitosan (CS) was used to immobilize AChE on SnO2 NPs–CGR–NF/GCE and to improve electronic transmission between AChE and SnO2 NPs–CGR–NF/GCE. NF was used as the protective membrane for the AChE biosensor. The SnO2 NPs–CGR–NF nanocomposites with excellent conductivity, catalysis and biocompatibility offered an extremely hydrophilic surface for AChE adhesion. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl with an apparent Michaelis–Menten constant value of 131μM. The biosensor detected methyl parathion in the linear range from 10−13 to 10−10 M and from 10−10 to 10−8 M. The biosensor detected carbofuran in the linear range from 10−12 to 10−10 M and from 10−10 to 10−8 M. The detection limits of methyl parathion and carbofuran were 5×10−14 M and 5×10−13 M, respectively. The biosensor exhibited low applied potential, high sensitivity and acceptable stability, thus providing a promising tool for analysis of pesticides.
Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies after measles infections
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Philani Mashazi , Phumlani Tetyana , Sibulelo Vilakazi , Tebello Nyokong
The detection of measles-specific primary antibodies (IgG) using electrochemical impedimetric immunosensors is reported. The optimum conditions for electrode saturation were reached after 40min for 1μgml−1 antibody concentrations. Surface roughness using AFM increased with each immobilization or antigen-antibody reaction step clearly confirming the surface modification and recognition between antigen and antibody. The human serum (HS) and new-born calf serum (NCS) spiked with antigen-specific antibody were studied to mimic the real sample analysis. The HS and NCS sera containing antibodies due to measles exhibited correlation between the increasing antibody serum concentrations and the charge-transfer resistance (electrochemically measured). This work clearly showed the potential use of impedance as the preferred electrochemical method for detecting measles-antibodies in label-free manner.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Philani Mashazi , Phumlani Tetyana , Sibulelo Vilakazi , Tebello Nyokong
The detection of measles-specific primary antibodies (IgG) using electrochemical impedimetric immunosensors is reported. The optimum conditions for electrode saturation were reached after 40min for 1μgml−1 antibody concentrations. Surface roughness using AFM increased with each immobilization or antigen-antibody reaction step clearly confirming the surface modification and recognition between antigen and antibody. The human serum (HS) and new-born calf serum (NCS) spiked with antigen-specific antibody were studied to mimic the real sample analysis. The HS and NCS sera containing antibodies due to measles exhibited correlation between the increasing antibody serum concentrations and the charge-transfer resistance (electrochemically measured). This work clearly showed the potential use of impedance as the preferred electrochemical method for detecting measles-antibodies in label-free manner.
Ultrasensitive electrochemical immunoassay for DNA methyltransferase activity and inhibitor screening based on methyl binding domain protein of MeCP2 and enzymatic signal amplification
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Huanshun Yin , Yunlei Zhou , Zhenning Xu , Mo Wang , Shiyun Ai
In this work, we fabricated a novel electrochemical immunosensor for detection of DNA methylation, analysis of DNA MTase activity and screening of MTase inhibitor. The immunosensor was on the basis of methyl binding domain protein of MeCP2 as DNA CpG methylation recognization unit, anti-His tag antibody as “immuno-bridge” and horseradish peroxidase labeled immuneglobulin G functionalized gold nanoparticles (AuNPs–IgG–HRP) as signal amplification unit. In the presence of M. SssI MTase, the symmetrical sequence of 5′-CCGG-3′ was methylated and then recognized by MeCP2 protein. By the immunoreactions, anti-His tag antibody and AuNPs–IgG–HRP was captured on the electrode surface successively. Under the catalysis effect of HRP towards hydroquinone oxidized by H2O2, the electrochemical reduction signal of benzoquinone was used to analyze M. SssI MTase activity. The electrochemical reduction signal demonstrated a wide linear relationship with M. SssI concentration ranging from 0.05unit/mL to 90unit/mL, achieving a detection limit of 0.017unit/mL (S/N=3). The most important advantages of this method were its high sensitivity and good selectivity, which enabled the detection of even one-base mismatched sequence. In addition, we also verified that the developed method could be applied for screening the inhibitors of DNA MTase and for developing new anticancer drugs.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Huanshun Yin , Yunlei Zhou , Zhenning Xu , Mo Wang , Shiyun Ai
In this work, we fabricated a novel electrochemical immunosensor for detection of DNA methylation, analysis of DNA MTase activity and screening of MTase inhibitor. The immunosensor was on the basis of methyl binding domain protein of MeCP2 as DNA CpG methylation recognization unit, anti-His tag antibody as “immuno-bridge” and horseradish peroxidase labeled immuneglobulin G functionalized gold nanoparticles (AuNPs–IgG–HRP) as signal amplification unit. In the presence of M. SssI MTase, the symmetrical sequence of 5′-CCGG-3′ was methylated and then recognized by MeCP2 protein. By the immunoreactions, anti-His tag antibody and AuNPs–IgG–HRP was captured on the electrode surface successively. Under the catalysis effect of HRP towards hydroquinone oxidized by H2O2, the electrochemical reduction signal of benzoquinone was used to analyze M. SssI MTase activity. The electrochemical reduction signal demonstrated a wide linear relationship with M. SssI concentration ranging from 0.05unit/mL to 90unit/mL, achieving a detection limit of 0.017unit/mL (S/N=3). The most important advantages of this method were its high sensitivity and good selectivity, which enabled the detection of even one-base mismatched sequence. In addition, we also verified that the developed method could be applied for screening the inhibitors of DNA MTase and for developing new anticancer drugs.
Graphical abstract
A label-free electrochemiluminescence cytosensors for specific detection of early apoptosis
28 May 2013,
17:37:35
Publication date: 15 November
2013
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Lin Zhang , Jin-Huan Jiang , Jian-Jun Luo , Lu Zhang , Ji-Ye Cai , Jiu-Wei Teng , Pei-Hui Yang
A novel electrochemiluminescence (ECL) cytosensors was developed for the detection of early apoptotic cells by the specific interaction between Annexin V and phosphatidylserine(PS) based on ECL signal of CdS-QDs. Immobilization of Annexin V on a L-cysteine-capped CdS-QDs/Polyaniline nanofibers (PANI-NF) resulted in the stable and high loading of Annexin V on the sensor surface and the possibility of sensitivity enhancement. Early apoptotic cells showed an increased exposure of PS on the cell membrane caused by physiological and pathological response reactions, leading to a strong interaction between the apoptotic cells and the sensor surface, which could be probed by the ECL. Using a real of early apoptotic HepG2 cell induced by resveratrol (RVL), the proposed novel strategy has demonstrated its simplicity, high sensitivity, good selectivity and high reproducibility and label-free capability which might hold a great potential for rapid detection of cell apoptosis and drug screening. The results from this approach have showed good agreement with those obtained using inverted microscope, flow cytometry(FCM) and Atomic force microscopy(AFM). The linear range for early apoptotic cells detection ranged from 500 to 1.0×106 cellsmL−1 with a detection limit of 500 cellsmL−1. The reported strategy has provided a promising platform for highly sensitive cytosensing and convenient screening of some clinically anticancer drugs.
Source:Biosensors and Bioelectronics, Volume 49
Author(s): Lin Zhang , Jin-Huan Jiang , Jian-Jun Luo , Lu Zhang , Ji-Ye Cai , Jiu-Wei Teng , Pei-Hui Yang
A novel electrochemiluminescence (ECL) cytosensors was developed for the detection of early apoptotic cells by the specific interaction between Annexin V and phosphatidylserine(PS) based on ECL signal of CdS-QDs. Immobilization of Annexin V on a L-cysteine-capped CdS-QDs/Polyaniline nanofibers (PANI-NF) resulted in the stable and high loading of Annexin V on the sensor surface and the possibility of sensitivity enhancement. Early apoptotic cells showed an increased exposure of PS on the cell membrane caused by physiological and pathological response reactions, leading to a strong interaction between the apoptotic cells and the sensor surface, which could be probed by the ECL. Using a real of early apoptotic HepG2 cell induced by resveratrol (RVL), the proposed novel strategy has demonstrated its simplicity, high sensitivity, good selectivity and high reproducibility and label-free capability which might hold a great potential for rapid detection of cell apoptosis and drug screening. The results from this approach have showed good agreement with those obtained using inverted microscope, flow cytometry(FCM) and Atomic force microscopy(AFM). The linear range for early apoptotic cells detection ranged from 500 to 1.0×106 cellsmL−1 with a detection limit of 500 cellsmL−1. The reported strategy has provided a promising platform for highly sensitive cytosensing and convenient screening of some clinically anticancer drugs.
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