The new Olympus FluoView FVMPE-RS, a
dedicated multiphoton microscope system, enables high-precision, ultra-fast
scanning and stimulation, allowing researchers to see deep within specimens,
take measurements at the highest speeds, and capture images, even when working
under the most demanding conditions.
With its high speed and precision performance,
the FVMPE-RS is designed for electrophysiology and optogenetics studies. It is
also a good match for applications such as high-speed calcium and in vivo
imaging, peristalsis and blood flow studies, mosaic imaging, connectomics and
functional brain imaging, stem cell research, and any field that requires precise
colocalisation, uncaging, simultaneous imaging/stimulation, extensive real-time
signal processing, or multipoint mapping. Its design offers ready adaptability
for researchers who design their own custom-built systems as well.
The precision timing on the system allows for
microsecond repeatability and control of multiple imaging and stimulation
protocols, as well as millisecond repeatability over days of time-lapse
imaging. Complex multi-position imaging or optogenetic stimulation protocols
can be accomplished using the advanced stage control and sequence manager.
The new system offers unmatched speed. The
FVMPE-RS captures 438 frames per second (fps) at 512x32, the fastest rate
commercially available. It also captures full-frame, 512x512 images at 30 fps
without any reduction of the field of view, a critical feature for many
functional imaging studies. Its scanner unit combines a newly designed
resonance scanner with a galvanometer scanner to provide both speed and
excellent definition. The resonance scanner offers an exclusive non-linear
sampling method for smooth imaging without jitter. An optional third
galvanometer scanner is also available for imaging with simultaneous 3D
stimulation or uncaging. The system’s multipoint mapping capability allows ultra-high-speed
measurement of rapid fluctuations in groups of cells for functional imaging
studies, 3D intensity measurement or mapping with high signal-to-noise
performance.
In addition to speed, the FVMPE-RS offers
optimal performance when imaging live cells and tissues. It provides
multicolour, multiphoton excitation and imaging with a choice of lasers, along
with an industry-first four-axis auto-alignment capability for precise
colocalisation and coalignment without pixel shift. With its multiple-laser-line
performance, researchers can work with two IR laser lines simultaneously while
stimulating the sample with visible light. The system delivers sharp images
with clear separation of fluorescence proteins for analysis.
The high-sensitivity system offers high
efficiency multiphoton excitation at wavelengths of 1300nm, with the ability to
support wavelengths of up to 1600nm. Objectives that offer working distances of
2, 4 or 8mm deliver super-deep imaging. Light collection efficiency is further
improved by the system’s optimised light path. In Deep Focus Mode, researchers
can tune lasers precisely for deep-tissue imaging to achieve signal
improvements of 20 percent or more over comparable systems. An optional
high-sensitivity cooled gallium arsenide phosphide (GaAsP) detector unit
further enhances sensitivity while minimising electrical noise, making the most
out of the system’s high-speed capabilities.
The new system’s multichannel analogue box for
triggering, synchronisation and signal input provides additional support for
advanced electrophysiology studies and other applications requiring external
device triggering. The advanced optional nosepiece piezo drive provides greater
rigidity and faster z-plane stabilisation to ensure accurate and repeatable high-speed
focus performance.
The FVMPE-RS is designed to be simple to use
and require minimal alignment, thanks to its robust auto-alignment system and
easy touch-panel control. Enhanced temperature stability and a highly rigid
frame make it an ideal foundation for live cell experiments.
A dedicated multiphoton system, the FVMPE-RS is
compatible with Olympus’ industry-leading range of multiphoton specialty
objectives, including the multi-award-winning 4mm and 8mm SCALEVIEW objectives
and Olympus’ high-performance, 25x dedicated multiphoton objectives.
The system will be sold as part of the full
line of Olympus advanced imaging microscope systems, including the FV1200 and
FV1200MPE family of microscope systems. For more information on the Olympus
FVMPE-RS multiphoton system, visit www.microscopy.olympus.eu
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